ADCY4 (NM_139247) Human Tagged ORF Clone

CAT#: RG212857

ADCY4 (tGFP-tagged) - Human adenylate cyclase 4 (ADCY4), transcript variant 2

Product Images

Circular map for RG212857

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ADCY4
• Synonyms: AC4
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG212857 representing NM_139247
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCGCCTCTTCAGCCCCCGGCCGCCCCCCAGCGAAGACCTCTTCTACGAGACCTACTACAGCCTGA
  GCCAGCAGTACCCGCTGCTGCTGCTGCTGCTGGGGATCGTGCTCTGTGCGCTCGCGGCGCTGCTCGCAGT
  GGCCTGGGCCAGCGGCAGGGAGCTGACCTCAGACCCGAGCTTCCTGACCACTGTGCTGTGCGCGCTGGGC
  GGCTTCTCGCTGCTGCTGGGCCTCGCTTCCCGGGAGCAGCGACTGCAGCGCTGGACGCGTCCCCTGTCCG
  GCTTGGTATGGGTCGCGCTGCTAGCGCTAGGCCACGCCTTCCTGTTCACCGGGGGCGTGGTGAGCGCCTG
  GGACCAGGTGTCCTATTTTCTCTTCGTCATCTTCACGGCGTATGCCATGCTGCCCTTGGGCATGCGGGAC
  GCCGCCGTCGCGGGCCTCGCCTCCTCACTCTCGCATCTGCTGGTCCTCGGGCTGTATCTTGGGCCACAGC
  CGGACTCACGGCCTGCACTGCTGCCGCAGTTGGCAGCAAACGCAGTGCTGTTCCTGTGCGGGAACGTGGC
  AGGAGTGTACCACAAGGCGCTGATGGAGCGCGCCCTGCGGGCCACGTTCCGGGAGGCACTCAGCTCCCTG
  CACTCACGCCGGCGGCTGGACACCGAGAAGAAGCACCAGGAACACCTTCTCTTGTCCATCCTTCCTGCCT
  ACCTGGCCCGAGAGATGAAGGCAGAGATCATGGCACGGCTGCAGGCAGGACAGGGGTCACGGCCAGAGAG
  CACTAACAATTTCCACAGCCTCTATGTCAAGAGGCACCAGGGAGTCAGCGTGCTGTATGCTGACATCGTG
  GGCTTCACGCGGCTGGCCAGCGAGTGTTCCCCTAAGGAGCTGGTGCTCATGCTCAATGAGCTCTTTGGCA
  AGTTCGACCAGATTGCCAAGGAGCATGAATGCATGCGGATCAAGATCCTGGGGGACTGTTACTACTGTGT
  CTCTGGGCTGCCACTCTCACTGCCAGACCATGCCATCAACTGCGTGCGCATGGGCCTGGACATGTGCCGG
  GCCATCAGGAAACTGCGGGCAGCCACTGGCGTGGACATCAACATGCGTGTGGGCGTGCACTCAGGCAGCG
  TACTGTGTGGAGTCATCGGGCTGCAGAAGTGGCAGTACGACGTTTGGTCACATGATGTCACACTGGCTAA
  CCACATGGAGGCAGGCGGTGTACCAGGGCGAGTGCACATCACAGGGGCTACCCTGGCCCTGCTGGCAGGG
  GCTTATGCTGTGGAGGACGCAGGCATGGAGCATCGGGACCCCTACCTTCGGGAGCTAGGGGAGCCTACCT
  ATCTGGTCATCGATCCACGGGCAGAGGAGGAGGATGAGAAGGGCACTGCAGGAGGCTTGCTGTCCTCGCT
  TGAGGGCCTCAAGATGCGTCCATCACTGCTGATGACCCGTTACCTGGAGTCCTGGGGCGCAGCCAAGCCT
  TTTGCCCACCTGAGCCACGGAGACAGCCCTGTGTCCACCTCCACCCCTCTCCCGGAGAAGACCCTGGCTT
  CCTTCAGCACCCAGTGGAGCCTGGATCGGAGCCGTACCCCCCGGGGACTAGATGATGAACTGGACACCGG
  GGATGCCAAGTTCTTCCAGGTCATTGAGCAGCTCAACTCGCAGAAACAGTGGAAGCAGTCGAAGGACTTC
  AACCCACTGACACTGTACTTCAGAGAGAAGGAGATGGAGAAAGAGTACCGACTCTCTGCAATCCCCGCCT
  TCAAATACTATGAAGCCTGCACCTTCCTGGTTTTTCTCTCCAACTTCATCATCCAGATGCTAGTGACAAA
  CAGGCCCCCAGCTCTGGCCATCACGTATAGCATCACCTTCCTCCTCTTCCTCCTCATCCTTTTTGTCTGC
  TTCTCAGAGGACCTGATGAGGTGTGTCCTGAAAGGCCCCAAGATGCTGCACTGGCTGCCTGCACTGTCTG
  GCCTGGTGGCCACACGACCAGGACTGAGAATAGCCTTGGGCACCGCCACCATCCTCCTTGTCTTTGCCAT
  GGCCATTACCAGCCTGTTCTTCTTCCCAACATCATCAGACTGCCCTTTCCAAGCTCCCAATGTGTCCTCC
  ATGATTTCCAACCTCTCCTGGGAGCTCCCTGGGTCTCTGCCTCTCATCAGTGTCCCATACTCCATGCACT
  GCTGCACGCTGGGCTTCCTCTCCTGCTCCCTCTTTCTGCACATGAGCTTCGAGCTGAAGCTGCTGCTGCT
  CCTGCTGTGGCTGGCGGCATCCTGCTCCCTCTTCCTGCACTCCCATGCCTGGCTGTCGGAATGCCTCATC
  GTCCGCCTCTATCTGGGCCCCTTGGACTCCAGGCCCGGAGTGCTGAAGGAGCCCAAACTGATGGGTGCTA
  TCTCCTTCTTCATCTTCTTCTTCACCCTCCTTGTCCTGGCTCGCCAGAATGAGTACTACTGCCGCCTGGA
  CTTCCTGTGGAAGAAGAAGCTGAGGCAGGAGAGGGAGGAGACAGAGACGATGGAGAACCTGACTCGGCTG
  CTCTTGGAGAACGTGCTCCCTGCACACGTGGCCCCCCAGTTCATTGGCCAGAACCGGCGCAACGAGGATC
  TCTACCACCAGTCCTATGAATGCGTTTGTGTCCTCTTCGCCTCAGTCCCAGACTTCAAGGAGTTCTACTC
  TGAATCCAACATCAATCATGAGGGCCTAGAGTGTCTGAGGCTGCTCAATGAGATAATTGCTGATTTTGAT
  GAGCTGCTCTCCAAGCCCAAGTTCAGTGGGGTGGAGAAGATCAAGACCATCGGCAGCACCTACATGGCAG
  CCACAGGCTTAAATGCCACCTCTGGACAGGATGCACAACAGGATGCTGAACGGAGCTGCAGCCACCTTGG
  CACTATGGTGGAATTTGCCGTGGCCCTGGGGTCTAAGCTGGACGTCATCAACAAGCATTCATTCAACAAC
  TTCCGCCTGCGAGTGGGGTTGAACCATGGACCCGTAGTAGCTGGAGTTATTGGGGCCCAGAAGCCGCAAT
  ATGACATTTGGGGCAACACAGTGAACGTGGCCAGCCGCATGGAGAGTACAGGAGTCCTTGGCAAAATCCA
  AGTGACTGAGGAGACAGCATGGGCCCTACAGTCCCTGGGCTACACCTGCTACAGCCGGGGTGTCATCAAG
  GTGAAAGGCAAAGGGCAGCTCTGCACCTACTTCCTGAACACAGACTTGACACGAACTGGACCTCCTTCAG
  CTACCCTAGGC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG212857 representing NM_139247
  Red=Cloning site Green=Tags(s)
  
  MARLFSPRPPPSEDLFYETYYSLSQQYPLLLLLLGIVLCALAALLAVAWASGRELTSDPSFLTTVLCALG
  GFSLLLGLASREQRLQRWTRPLSGLVWVALLALGHAFLFTGGVVSAWDQVSYFLFVIFTAYAMLPLGMRD
  AAVAGLASSLSHLLVLGLYLGPQPDSRPALLPQLAANAVLFLCGNVAGVYHKALMERALRATFREALSSL
  HSRRRLDTEKKHQEHLLLSILPAYLAREMKAEIMARLQAGQGSRPESTNNFHSLYVKRHQGVSVLYADIV
  GFTRLASECSPKELVLMLNELFGKFDQIAKEHECMRIKILGDCYYCVSGLPLSLPDHAINCVRMGLDMCR
  AIRKLRAATGVDINMRVGVHSGSVLCGVIGLQKWQYDVWSHDVTLANHMEAGGVPGRVHITGATLALLAG
  AYAVEDAGMEHRDPYLRELGEPTYLVIDPRAEEEDEKGTAGGLLSSLEGLKMRPSLLMTRYLESWGAAKP
  FAHLSHGDSPVSTSTPLPEKTLASFSTQWSLDRSRTPRGLDDELDTGDAKFFQVIEQLNSQKQWKQSKDF
  NPLTLYFREKEMEKEYRLSAIPAFKYYEACTFLVFLSNFIIQMLVTNRPPALAITYSITFLLFLLILFVC
  FSEDLMRCVLKGPKMLHWLPALSGLVATRPGLRIALGTATILLVFAMAITSLFFFPTSSDCPFQAPNVSS
  MISNLSWELPGSLPLISVPYSMHCCTLGFLSCSLFLHMSFELKLLLLLLWLAASCSLFLHSHAWLSECLI
  VRLYLGPLDSRPGVLKEPKLMGAISFFIFFFTLLVLARQNEYYCRLDFLWKKKLRQEREETETMENLTRL
  LLENVLPAHVAPQFIGQNRRNEDLYHQSYECVCVLFASVPDFKEFYSESNINHEGLECLRLLNEIIADFD
  ELLSKPKFSGVEKIKTIGSTYMAATGLNATSGQDAQQDAERSCSHLGTMVEFAVALGSKLDVINKHSFNN
  FRLRVGLNHGPVVAGVIGAQKPQYDIWGNTVNVASRMESTGVLGKIQVTEETAWALQSLGYTCYSRGVIK
  VKGKGQLCTYFLNTDLTRTGPPSATLG
  
  SGPTRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_139247
• ORF Size: 3231 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_139247.4
• RefSeq Size: 3415 bp
• RefSeq ORF: 3234 bp
• Locus ID: 196883
• UniProt ID: Q8NFM4
• Cytogenetics: 14q12
• Domains: CYCc
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Calcium signaling pathway, Chemokine signaling pathway, Dilated cardiomyopathy, Gap junction, GnRH signaling pathway, Melanogenesis, Oocyte meiosis, Progesterone-mediated oocyte maturation, Purine metabolism, Taste transduction, Vascular smooth muscle contraction
• Gene Summary: This gene encodes a member of the family of adenylate cyclases, which are membrane-associated enzymes that catalyze the formation of the secondary messenger cyclic adenosine monophosphate (cAMP). Mouse studies show that adenylate cyclase 4, along with adenylate cyclases 2 and 3, is expressed in olfactory cilia, suggesting that several different adenylate cyclases may couple to olfactory receptors and that there may be multiple receptor-mediated mechanisms for the generation of cAMP signals. Alternative splicing results in transcript variants. [provided by RefSeq, Nov 2010]