FOXA2 (NM_153675) Human Tagged ORF Clone

CAT#: RG211408

FOXA2 (tGFP-tagged) - Human forkhead box A2 (FOXA2), transcript variant 2

Product Images

Circular map for RG211408

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: FOXA2
• Synonyms: HNF-3-beta; HNF3B; TCF3B
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG211408 representing NM_153675
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCACTCGGCTTCCAGTATGCTGGGAGCGGTGAAGATGGAAGGGCACGAGCCGTCCGACTGGAGCAGCT
  ACTATGCAGAGCCCGAGGGCTACTCCTCCGTGAGCAACATGAACGCCGGCCTGGGGATGAACGGCATGAA
  CACGTACATGAGCATGTCGGCGGCCGCCATGGGCAGCGGCTCGGGCAACATGAGCGCGGGCTCCATGAAC
  ATGTCGTCGTACGTGGGCGCTGGCATGAGCCCGTCCCTGGCGGGGATGTCCCCCGGCGCGGGCGCCATGG
  CGGGCATGGGCGGCTCGGCCGGGGCGGCTGGCGTGGCGGGCATGGGGCCGCACTTGAGTCCCAGCCTGAG
  CCCGCTCGGGGGGCAGGCGGCCGGGGCCATGGGCGGCCTGGCCCCCTACGCCAACATGAACTCCATGAGC
  CCCATGTACGGGCAGGCGGGCCTGAGCCGCGCCCGCGACCCCAAGACCTACAGGCGCAGCTACACGCACG
  CAAAGCCGCCCTACTCGTACATCTCGCTCATCACCATGGCCATCCAGCAGAGCCCCAACAAGATGCTGAC
  GCTGAGCGAGATCTACCAGTGGATCATGGACCTCTTCCCCTTCTACCGGCAGAACCAGCAGCGCTGGCAG
  AACTCCATCCGCCACTCGCTCTCCTTCAACGACTGTTTCCTGAAGGTGCCCCGCTCGCCCGACAAGCCCG
  GCAAGGGCTCCTTCTGGACCCTGCACCCTGACTCGGGCAACATGTTCGAGAACGGCTGCTACCTGCGCCG
  CCAGAAGCGCTTCAAGTGCGAGAAGCAGCTGGCGCTGAAGGAGGCCGCAGGCGCCGCCGGCAGCGGCAAG
  AAGGCGGCCGCCGGGGCCCAGGCCTCACAGGCTCAACTCGGGGAGGCCGCCGGGCCGGCCTCCGAGACTC
  CGGCGGGCACCGAGTCGCCTCACTCGAGCGCCTCCCCGTGCCAGGAGCACAAGCGAGGGGGCCTGGGAGA
  GCTGAAGGGGACGCCGGCTGCGGCGCTGAGCCCCCCAGAGCCGGCGCCCTCTCCCGGGCAGCAGCAGCAG
  GCCGCGGCCCACCTGCTGGGCCCGCCCCACCACCCGGGCCTGCCGCCTGAGGCCCACCTGAAGCCGGAAC
  ACCACTACGCCTTCAACCACCCGTTCTCCATCAACAACCTCATGTCCTCGGAGCAGCAGCACCACCACAG
  CCACCACCACCACCAGCCCCACAAAATGGACCTCAAGGCCTACGAACAGGTGATGCACTACCCCGGCTAC
  GGTTCCCCCATGCCTGGCAGCTTGGCCATGGGCCCGGTCACGAACAAAACGGGCCTGGACGCCTCGCCCC
  TGGCCGCAGATACCTCCTACTACCAGGGGGTGTACTCCCGGCCCATTATGAACTCCTCT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG211408 representing NM_153675
  Red=Cloning site Green=Tags(s)
  
  MHSASSMLGAVKMEGHEPSDWSSYYAEPEGYSSVSNMNAGLGMNGMNTYMSMSAAAMGSGSGNMSAGSMN
  MSSYVGAGMSPSLAGMSPGAGAMAGMGGSAGAAGVAGMGPHLSPSLSPLGGQAAGAMGGLAPYANMNSMS
  PMYGQAGLSRARDPKTYRRSYTHAKPPYSYISLITMAIQQSPNKMLTLSEIYQWIMDLFPFYRQNQQRWQ
  NSIRHSLSFNDCFLKVPRSPDKPGKGSFWTLHPDSGNMFENGCYLRRQKRFKCEKQLALKEAAGAAGSGK
  KAAAGAQASQAQLGEAAGPASETPAGTESPHSSASPCQEHKRGGLGELKGTPAAALSPPEPAPSPGQQQQ
  AAAHLLGPPHHPGLPPEAHLKPEHHYAFNHPFSINNLMSSEQQHHHSHHHHQPHKMDLKAYEQVMHYPGY
  GSPMPGSLAMGPVTNKTGLDASPLAADTSYYQGVYSRPIMNSS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_153675
• ORF Size: 1389 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_153675.1, NP_710141.1
• RefSeq Size: 2230 bp
• RefSeq ORF: 1374 bp
• Locus ID: 3170
• UniProt ID: Q9Y261
• Cytogenetics: 20p11.21
• Protein Families: Embryonic stem cells, ES Cell Differentiation/IPS, Induced pluripotent stem cells, Transcription Factors
• Protein Pathways: Maturity onset diabetes of the young
• Gene Summary: This gene encodes a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific genes such as albumin and transthyretin, and they also interact with chromatin. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver. This gene has been linked to sporadic cases of maturity-onset diabetes of the young. Transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Oct 2008]