D Amino Acid Oxidase (DAO) (NM_001917) Human Tagged ORF Clone

CAT#: RG206768

DAO (tGFP-tagged) - Human D-amino-acid oxidase (DAO)

Product Images

Circular map for RG206768

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: D Amino Acid Oxidase
• Synonyms: DAAO; DAMOX; OXDA
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG206768 representing NM_001917
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGTGTGGTGGTGATTGGAGCAGGAGTCATCGGGCTGTCCACCGCCCTCTGCATCCATGAGCGCTACC
  ACTCAGTCCTGCAGCCACTGGACATAAAGGTCTACGCGGACCGCTTCACCCCACTCACCACCACCGACGT
  GGCTGCCGGCCTCTGGCAGCCCTACCTTTCTGACCCCAACAACCCACAGGAGGCGGACTGGAGCCAACAG
  ACCTTTGACTATCTCCTGAGCCATGTCCATTCTCCCAACGCTGAAAACCTGGGCCTGTTCCTAATCTCGG
  GCTACAACCTCTTCCATGAAGCCATTCCGGACCCTTCCTGGAAGGACACAGTTCTGGGATTTCGGAAGCT
  GACCCCCAGAGAGCTGGATATGTTCCCAGATTACGGCTATGGCTGGTTCCACACAAGCCTAATTCTGGAG
  GGAAAGAACTATCTACAGTGGCTGACTGAAAGGTTAACTGAGAGGGGAGTGAAGTTCTTCCAGCGGAAAG
  TGGAGTCTTTTGAGGAGGTGGCAAGAGAAGGCGCAGACGTGATTGTCAACTGCACTGGGGTATGGGCTGG
  GGCGCTACAACGAGACCCCCTGCTGCAGCCAGGCCGGGGGCAGATCATGAAGGTGGACGCCCCTTGGATG
  AAGCACTTCATTCTCACCCATGACCCAGAGAGAGGCATCTACAATTCCCCGTACATCATCCCAGGGACCC
  AGACAGTTACTCTTGGAGGCATCTTCCAGTTGGGAAACTGGAGTGAACTAAACAATATCCAGGACCACAA
  CACCATTTGGGAAGGCTGCTGCAGACTGGAGCCCACACTGAAGAATGCAAGAATTATTGGTGAACGAACT
  GGCTTCCGGCCAGTACGCCCCCAGATTCGGCTAGAAAGAGAACAGCTTCGCACTGGACCTTCAAACACAG
  AGGTCATCCACAACTATGGCCATGGAGGCTACGGGCTCACCATCCACTGGGGATGTGCCCTGGAGGCAGC
  CAAGCTCTTTGGGAGAATCCTGGAAGAAAAGAAATTGTCCAGAATGCCACCATCCCACCTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG206768 representing NM_001917
  Red=Cloning site Green=Tags(s)
  
  MRVVVIGAGVIGLSTALCIHERYHSVLQPLDIKVYADRFTPLTTTDVAAGLWQPYLSDPNNPQEADWSQQ
  TFDYLLSHVHSPNAENLGLFLISGYNLFHEAIPDPSWKDTVLGFRKLTPRELDMFPDYGYGWFHTSLILE
  GKNYLQWLTERLTERGVKFFQRKVESFEEVAREGADVIVNCTGVWAGALQRDPLLQPGRGQIMKVDAPWM
  KHFILTHDPERGIYNSPYIIPGTQTVTLGGIFQLGNWSELNNIQDHNTIWEGCCRLEPTLKNARIIGERT
  GFRPVRPQIRLEREQLRTGPSNTEVIHNYGHGGYGLTIHWGCALEAAKLFGRILEEKKLSRMPPSHL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001917
• ORF Size: 1041 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001917.5
• RefSeq Size: 1576 bp
• RefSeq ORF: 1044 bp
• Locus ID: 1610
• UniProt ID: P14920
• Cytogenetics: 12q24.11
• Domains: DAO
• Protein Families: Druggable Genome
• Protein Pathways: Arginine and proline metabolism, D-Arginine and D-ornithine metabolism, Glycine, serine and threonine metabolism, Metabolic pathways
• Gene Summary: This gene encodes the peroxisomal enzyme D-amino acid oxidase. The enzyme is a flavoprotein which uses flavin adenine dinucleotide (FAD) as its prosthetic group. Its substrates include a wide variety of D-amino acids, but it is inactive on the naturally occurring L-amino acids. Its biological function is not known; it may act as a detoxifying agent which removes D-amino acids that accumulate during aging. In mice, it degrades D-serine, a co-agonist of the NMDA receptor. This gene may play a role in the pathophysiology of schizophrenia. [provided by RefSeq, Jul 2008]