ZNF480 (NM_144684) Human Tagged ORF Clone

CAT#: RG205885

ZNF480 (tGFP-tagged) - Human zinc finger protein 480 (ZNF480)

Product Images

Circular map for RG205885

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ZNF480
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG205885 representing NM_144684
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCTTCCTCAGGGACACTTAACATTCAGGGACGTGGCCATAGAATTCTCTCAGGCGGAGTGGAAAT
  GCCTGGACCCTGCACAGAGGGCTTTATACAAGGATGTGATGTTGGAGAACTACAGGAACCTGGTCTCCCT
  GGGAATCTCTCTTCCTGACCTGAATATTAACTCCATGTTGGAGCAAAGGAGGGAGCCCTGGTCTGGTGAG
  AGTGAAGTGAAAATAGCAAAAAATTCAGATGGGAGGGAGTGCATCAAAGGTGTGAACACAGGGAGCAGCT
  ATGCATTGGGAAGCAATGCAGAAGACAAACCAATTAAAAAACAACTTGGAGTATCCTTTCACTTACATCT
  GTCTGAACTGGAGCTATTTCCAGATGAAAGGGTAATAAATGGATGTAATCAAGTTGAAAACTTTATCAAC
  CACAGTTCCTCTGTTTCCTGTCTTCAAGAAATGTCTTCCAGTGTCAAAACCCCCATTTTTAATAGGAATG
  ATTTTGATGATTCTTCATTTCTCCCACAAGAACAGAAAGTACACCTTAGAGAAAAACCTTATGAATGTAA
  TGAGCATAGCAAAGTCTTTAGAGTATCTTCCAGCCTTACTAAACATCAAGTAATCCATACTGTAGAGAAA
  CCTTACAAATGTAATTCATGCGGCAAGGTCTTTAGTCGCAATTCGCACCTTGCAGAACATTGTAGAATTC
  ATACTGGAGAGAAACCTTACAAATGTAATGTCTGTGGCAAGGTTTTTAGTTACAATTCAAACTTTGCACG
  ACATCAAAGAATTCATACCAGAGAGAAGCCGTATGAATGTAATGAATGTGGTAAAGTCTTCAGTAATAAT
  TCTTACCTTGCACGACATCAAAGAATTCATGCTGAAGAGAAACCTTACAAATGTAATGAATGTGGTAAAG
  GCTTCAGTCATAAGTCATCTCTAGCAAATCATTGGAGAATTTATACTGGAGAGAAGCCTTACAAATGTGA
  TGAATGTGGCAAGGCCTTCTATAGGATTGCGCTCCTTGTACGACATCAGAAAATTCATACTGGAGAGAAA
  CCTTACAAATGTAATGAATGTGGAAAGGTCTTTATTCAAAATTCGCACCTAGCACAACATTGGAGAATTC
  ATACAGGAGAGAAACCTTACAAATGTAATGAATGTGGAAAAGTATTTAATCAACTTTCAAATCTTGCACG
  ACATCGAAGAATTCATACTGGAGAGAAGCCTTACAAATGTAATGAATGTGGTAAAGCATTTAGTGAGTAT
  TCAGGCCTTTCAGCCCATCTTGTAATCCACACTGGAGAGAAGCCTTACAAATGTAGTGAATGTGGCAAGG
  CATTCAGACACAAGTTATCACTAACCAATCATCAGAGAATCCATACTGGAGAAAGACCTTACAAATGTAA
  TGAATGTGGCAAGGTCTTCAATCGAATTGCACACCTTGCACGACATCGGAAAATTCATACTGGAGAGAAA
  CCTTACAAATGTAATGAGTGTGGCAAGGCCTTTAGTCGCATTTCATACCTAGCACAACATTGGACAATTC
  ATATGGGA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG205885 representing NM_144684
  Red=Cloning site Green=Tags(s)
  
  MALPQGHLTFRDVAIEFSQAEWKCLDPAQRALYKDVMLENYRNLVSLGISLPDLNINSMLEQRREPWSGE
  SEVKIAKNSDGRECIKGVNTGSSYALGSNAEDKPIKKQLGVSFHLHLSELELFPDERVINGCNQVENFIN
  HSSSVSCLQEMSSSVKTPIFNRNDFDDSSFLPQEQKVHLREKPYECNEHSKVFRVSSSLTKHQVIHTVEK
  PYKCNSCGKVFSRNSHLAEHCRIHTGEKPYKCNVCGKVFSYNSNFARHQRIHTREKPYECNECGKVFSNN
  SYLARHQRIHAEEKPYKCNECGKGFSHKSSLANHWRIYTGEKPYKCDECGKAFYRIALLVRHQKIHTGEK
  PYKCNECGKVFIQNSHLAQHWRIHTGEKPYKCNECGKVFNQLSNLARHRRIHTGEKPYKCNECGKAFSEY
  SGLSAHLVIHTGEKPYKCSECGKAFRHKLSLTNHQRIHTGERPYKCNECGKVFNRIAHLARHRKIHTGEK
  PYKCNECGKAFSRISYLAQHWTIHMG
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_144684
• ORF Size: 1548 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_144684.1, NP_653285.1
• RefSeq Size: 4732 bp
• RefSeq ORF: 1608 bp
• Locus ID: 147657
• UniProt ID: Q8WV37
• Cytogenetics: 19q13.41
• Domains: KRAB, zf-C2H2
• Protein Families: Transcription Factors
• Gene Summary: Involved in transcriptional regulation as an activator.[UniProtKB/Swiss-Prot Function]