SFRP4 (NM_003014) Human Tagged ORF Clone

CAT#: RG205365

SFRP4 (tGFP-tagged) - Human secreted frizzled-related protein 4 (SFRP4)

Product Images

Circular map for RG205365

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: SFRP4
• Synonyms: FRP-4; FRPHE; FRZB-2; PYL; sFRP-4
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG205365 representing NM_003014
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGTGGCTGGCTGCGCTCCGAGCTGCGGAGTCCGGGACTGGAGCTGCCCGGGCGGGTTCGCGCCCC
  GAAGGCTGAGAGCTGGCGCTGCTCGTGCCCTGTGTGCCAGACGGCGGAGCTCCGCGGCCGGACCCCGCGG
  CCCCGTTTTGCTGCCGACTGGAGTTTGGGGGAAGAAACTCTCCTGCGCCCCAGAGGATTTCTTCCTCGGC
  GAAGGGACAGCGAAAGATGAGGGTGGCAGGAAGAGAAGGGCGCTTTCTGTCTGCCGGGGTCGCAGCGCGA
  GAGGGCAGTGCCATGTTCCTCTCCATCCTAGTGGCGCTGTGCCTGTGGCTGCACCTGGCGCTGGCGTGCG
  CGGCGCGCCCTGCGAGGCGGTGCGCATCCCTATGTGCCGGCACATGCCCTGGAACATCACGCGGATGCCC
  AACCACCTGCACCACAGCACGCAGGAGAACGCCATCCTGGCCATCGAGCAGTACGAGGAGCTGGTGGACG
  TGAACTGCAGCGCCGTGCTGCGCTTCTTCCTCTGTGCCATGTACGCGCCCATTTGCACCCTGGAGTTCCT
  GCACGACCCTATCAAGCCGTGCAAGTCGGTGTGCCAACGCGCGCGCGACGACTGCGAGCCCCTCATGAAG
  ATGTACAACCACAGCTGGCCCGAAAGCCTGGCCTGCGACGAGCTGCCTGTCTATGACCGTGGCGTGTGCA
  TCTCGCCTGAAGCCATCGTCACGGACCTCCCGGAGGATGTTAAGTGGATAGACATCACACCAGACATGAT
  GGTACAGGAAAGGCCTCTTGATGTTGACTGTAAACGCCTAAGCCCCGATCGGTGCAAGTGTAAAAAGGTG
  AAGCCAACTTTGGCAACGTATCTCAGCAAAAACTACAGCTATGTTATTCATGCCAAAATAAAAGCTGTGC
  AGAGGAGTGGCTGCAATGAGGTCACAACGGTGGTGGATGTAAAAGAGATCTTCAAGTCCTCATCACCCAT
  CCCTCGAACTCAAGTCCCGCTCATTACAAATTCTTCTTGCCAGTGTCCACACATCCTGCCCCATCAAGAT
  GTTCTCATCATGTGTTACGAGTGGCGTTCAAGGATGATGCTTCTTGAAAATTGCTTAGTTGAAAAATGGA
  GAGATCAGCTTAGTAAAAGATCCATACAGTGGGAAGAGAGGCTGCAGGAACAGCGGAGAACAGTTCAGGA
  CAAGAAGAAAACAGCCGGGCGCACCAGTCGTAGTAATCCCCCCAAACCAAAGGGAAAGCCTCCTGCTCCC
  AAACCAGCCAGTCCCAAGAAGAACATTAAAACTAGGAGTGCCCGGAAGAGAACAAACCCGAAAAGAGTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG205365 representing NM_003014
  Red=Cloning site Green=Tags(s)
  
  MAVAGCAPSCGVRDWSCPGGFAPRRLRAGAARALCARRRSSAAGPRGPVLLPTGVWGKKLSCAPEDFFLG
  EGTAKDEGGRKRRALSVCRGRSARGQCHVPLHPSGAVPVAAPGAGVRGAPCEAVRIPMCRHMPWNITRMP
  NHLHHSTQENAILAIEQYEELVDVNCSAVLRFFLCAMYAPICTLEFLHDPIKPCKSVCQRARDDCEPLMK
  MYNHSWPESLACDELPVYDRGVCISPEAIVTDLPEDVKWIDITPDMMVQERPLDVDCKRLSPDRCKCKKV
  KPTLATYLSKNYSYVIHAKIKAVQRSGCNEVTTVVDVKEIFKSSSPIPRTQVPLITNSSCQCPHILPHQD
  VLIMCYEWRSRMMLLENCLVEKWRDQLSKRSIQWEERLQEQRRTVQDKKKTAGRTSRSNPPKPKGKPPAP
  KPASPKKNIKTRSARKRTNPKRV
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003014
• ORF Size: 1329 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003014.2, NP_003005.1
• RefSeq Size: 2820 bp
• RefSeq ORF: 1041 bp
• Locus ID: 6424
• UniProt ID: Q6FHJ7
• Cytogenetics: 7p14.1
• Domains: FRI, NTR
• Protein Families: Adult stem cells, Cancer stem cells, Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein, Stem cell relevant signaling - Wnt Signaling pathway
• Protein Pathways: Wnt signaling pathway
• Gene Summary: Secreted frizzled-related protein 4 (SFRP4) is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. The expression of SFRP4 in ventricular myocardium correlates with apoptosis related gene expression. [provided by RefSeq, Jul 2008]