Spermidine synthase (SRM) (NM_003132) Human Tagged ORF Clone

CAT#: RG201761

SRM (tGFP-tagged) - Human spermidine synthase (SRM)

Product Images

Circular map for RG201761

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Spermidine synthase
• Synonyms: PAPT; SPDSY; SPS1; SRML1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG201761 representing NM_003132
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCCCGGCCCCGACGGCCCCGCCGCCTCCGGCCCCGCCGCCATCCGCGAGGGCTGGTTCCGCGAGA
  CCTGCAGCCTGTGGCCCGGCCAGGCCCTGTCGCTGCAGGTGGAGCAGCTGCTCCACCACCGGCGCTCGCG
  CTACCAGGACATCCTCGTCTTCCGCAGTAAGACCTATGGCAACGTGCTGGTGTTGGACGGTGTCATCCAG
  TGCACGGAGAGAGACGAGTTCTCCTACCAGGAGATGATCGCCAACCTGCCTCTCTGCAGCCACCCCAACC
  CGCGAAAGGTGCTGATCATCGGGGGCGGAGATGGAGGTGTCCTGCGGGAGGTGGTGAAGCACCCCTCCGT
  GGAGTCCGTGGTCCAGTGTGAGATCGACGAGGATGTCATCCAAGTCTCCAAGAAGTTCCTGCCAGGCATG
  GCCATTGGCTACTCTAGCTCGAAGCTGACCCTACATGTGGGTGACGGTTTTGAGTTCATGAAACAGAATC
  AGGATGCCTTCGACGTGATCATCACTGACTCCTCAGACCCCATGGGCCCCGCCGAAAGTCTCTTCAAGGA
  GTCCTATTACCAGCTCATGAAGACAGCCCTCAAGGAAGATGGTGTCCTCTGCTGCCAGGGCGAGTGCCAG
  TGGCTGCACCTGGACCTCATCAAGGAGATGCGGCAGTTCTGCCAGTCCCTGTTCCCCGTGGTGGCCTATG
  CCTACTGCACCATCCCCACCTACCCCAGCGGCCAGATCGGCTTCATGCTGTGCAGCAAGAACCCGAGCAC
  GAACTTCCAGGAGCCGGTGCAGCCGCTGACACAGCAGCAGGTGGCGCAGATGCAGCTGAAGTACTACAAC
  TCCGACGTGCACCGCGCCGCCTTTGTGCTGCCCGAGTTTGCCCGCAAGGCCCTGAATGATGTGAGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG201761 representing NM_003132
  Red=Cloning site Green=Tags(s)
  
  MEPGPDGPAASGPAAIREGWFRETCSLWPGQALSLQVEQLLHHRRSRYQDILVFRSKTYGNVLVLDGVIQ
  CTERDEFSYQEMIANLPLCSHPNPRKVLIIGGGDGGVLREVVKHPSVESVVQCEIDEDVIQVSKKFLPGM
  AIGYSSSKLTLHVGDGFEFMKQNQDAFDVIITDSSDPMGPAESLFKESYYQLMKTALKEDGVLCCQGECQ
  WLHLDLIKEMRQFCQSLFPVVAYAYCTIPTYPSGQIGFMLCSKNPSTNFQEPVQPLTQQQVAQMQLKYYN
  SDVHRAAFVLPEFARKALNDVS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003132
• ORF Size: 906 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003132.2, NP_003123.2
• RefSeq Size: 1273 bp
• RefSeq ORF: 909 bp
• Locus ID: 6723
• UniProt ID: P19623
• Cytogenetics: 1p36.22
• Domains: Spermine_synth
• Protein Pathways: Arginine and proline metabolism, beta-Alanine metabolism, Cysteine and methionine metabolism, Glutathione metabolism, Metabolic pathways
• Gene Summary: The polyamines putrescine, spermine, and spermidine are ubiquitous polycationic mediators of cell growth and differentiation. Spermidine synthase is one of four enzymes in the polyamine-biosynthetic pathway and carries out the final step of spermidine biosynthesis. This enzyme catalyzes the conversion of putrescine to spermidine using decarboxylated S-adenosylmethionine as the cofactor. [provided by RefSeq, Jul 2008]