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TJP1 (NM_001301025) Human Tagged ORF Clone
CAT#: RC240215
- TrueORF®
TJP1 (myc-DDK-tagged) - Human tight junction protein 1 (TJP1), transcript variant 3
ORF Plasmid: tGFP
"NM_001301025" in other vectors (2)
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Frequently bought together (5)
DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
USD 198.00
Other products for "TJP1"
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Symbol | TJP1 |
| Synonyms | ZO-1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC240215 representing NM_001301025
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGTACCAGAAATACCTGACGGTGCTGCAGATGGCCATCGGCGTCACCCCCTCCAACCGCGGCAGCC TCCTGCCGCTCAAGAGGAAGCTGTGGGTAACGCCATCCTCTGAAAATCCTAATGGTGCTACTTCTAGTGT CAGCCAAGGAAAACCCTCTTTAAGACGAATTAAAGGGAGATTACACAGAAGCAAAAGCCTTGATAGCATG GATTTCTGTGAGCTCACTAGCACAGCAATGGAGGAAACAGCTATATGGGAACAACATACAGTGACGCTTC ACAGGGCTCCTGGATTTGGATTTGGAATTGCAATATCTGGTGGACGAGATAATCCTCATTTTCAGAGTGG GGAAACGTCAATAGTGATTTCAGATGTGCTGAAAGGAGGACCAGCTGAAGGACAGCTACAGGAAAATGAC CGAGTTGCAATGGTTAACGGAGTTTCAATGGATAATGTTGAACATGCTTTTGCTGTTCAGCAACTAAGGA AAAGTGGGAAAAATGCAAAAATTACAATTAGAAGGAAGAAGAAAGTTCAAATACCAGTAAGTCGTCCTGA TCCTGAACCAGTATCTGATAATGAAGAAGATAGTTATGATGAGGAAATACATGATCCAAGAAGTGGCCGG AGTGGTGTGGTTAACAGAAGGAGTGAGAAGATTTGGCCGAGGGATAGAAGTGCAAGTAGAGAGAGGAGCT TGTCCCCGCGGTCAGACAGGCGGTCAGTGGCTTCCAGCCAGCCTGCTAAACCTACTAAAGTCACACTGGT GAAATCCCGGAAAAATGAAGAATATGGTCTTCGATTGGCAAGCCATATATTTGTTAAGGAAATTTCACAA GATAGTTTGGCAGCAAGAGATGGCAATATTCAAGAAGGTGATGTTGTATTGAAGATAAATGGTACTGTGA CAGAAAATATGTCATTGACAGATGCAAAGACATTGATAGAAAGGTCTAAAGGCAAATTAAAAATGGTAGT TCAAAGAGATGAACGGGCTACGCTATTGAATGTCCCTGATCTTTCTGACAGCATCCACTCTGCTAATGCC TCTGAGAGAGACGACATTTCAGAAATTCAGTCACTGGCATCAGATCATTCTGGTCGATCACACGATAGGC CTCCCCGCCGCAGCCGGTCACGATCTCCTGACCAGCGGTCAGAGCCTTCTGATCATTCCAGGCACTCGCC GCAGCAGCCAAGCAATGGCAGTCTCCGGAGTAGAGATGAAGAGAGAATTTCTAAACCTGGGGCTGTCTCA ACTCCTGTAAAGCATGCTGATGATCACACACCTAAAACAGTGGAAGAAGTTACAGTTGAAAGAAATGAGA AACAAACACCTTCTCTTCCAGAACCAAAGCCTGTGTATGCCCAAGTTGGGCAACCAGATGTGGATTTACC TGTCAGTCCATCTGATGGTGTCCTACCTAATTCAACTCATGAAGATGGGATTCTTCGGCCCAGCATGAAA TTGGTAAAATTCAGAAAAGGAGATAGTGTGGGTTTGCGGCTGGCTGGTGGAAATGATGTTGGAATATTTG TAGCTGGCGTTCTAGAAGATAGCCCTGCAGCCAAGGAAGGCTTAGAGGAAGGTGATCAAATTCTCAGGGT AAACAACGTAGATTTTACAAATATCATAAGAGAAGAAGCCGTCCTTTTCCTGCTTGACCTCCCTAAAGGA GAAGAAGTGACCATATTGGCTCAGAAGAAGAAGGATGTTTATCGTCGCATTGTAGAATCAGATGTAGGAG ATTCTTTCTATATTAGAACCCATTTTGAATATGAAAAGGAATCTCCCTATGGACTTAGTTTTAACAAAGG AGAGGTGTTCCGTGTTGTGGATACCTTGTACAATGGAAAACTGGGCTCTTGGCTTGCTATTCGAATTGGT AAAAATCATAAGGAGGTAGAACGAGGCATCATCCCTAATAAGAACAGAGCTGAGCAGCTAGCCAGTGTAC AGTATACACTTCCAAAAACAGCAGGCGGAGACCGTGCTGACTTCTGGAGATTCAGAGGTCTTCGCAGCTC CAAGAGAAATCTTCGAAAAAGCAGAGAGGATTTGTCCGCTCAGCCTGTTCAAACAAAGTTTCCAGCTTAT GAAAGAGTGGTTCTTCGAGAAGCTGGATTTCTGAGGCCTGTAACCATTTTTGGACCAATAGCTGATGTTG CCAGAGAAAAGCTGGCAAGAGAAGAACCAGATATTTATCAAATTGCAAAGAGTGAACCACGAGACGCTGG AACTGACCAACGTAGCTCTGGCATTATTCGCCTGCATACAATAAAGCAAATCATAGATCAAGACAAACAT GCTTTATTAGATGTAACACCAAATGCAGTTGATCGTCTTAACTATGCCCAGTGGTATCCAATTGTTGTAT TTCTTAACCCTGATTCTAAGCAAGGAGTAAAAACAATGAGAATGAGGTTATGTCCAGAATCTCGGAAAAG TGCCAGGAAGTTATACGAGCGATCTCATAAACTTCGTAAAAATAATCACCATCTTTTTACAACTACAATT AACTTAAATTCAATGAATGATGGTTGGTATGGTGCGCTGAAAGAAGCAATTCAACAACAGCAAAACCAGC TGGTATGGGTTTCCGAGGGAAAGGCGGATGGTGCTACAAGTGATGACCTTGATTTGCATGATGATCGTCT GTCCTACCTGTCAGCTCCAGGTAGTGAATACTCAATGTATAGCACGGACAGTAGACACACTTCTGACTAT GAAGACACAGACACAGAAGGCGGGGCCTACACTGATCAAGAACTAGATGAAACTCTTAATGATGAGGTTG GGACTCCACCGGAGTCTGCCATTACACGGTCCTCTGAGCCTGTAAGAGAGGACTCCTCTGGAATGCATCA TGAAAACCAAACATATCCTCCTTACTCACCACAAGCGCAGCCACAACCAATTCATAGAATAGACTCCCCT GGATTTAAGCCAGCCTCTCAACAGAAAGCAGAAGCTTCATCTCCAGTCCCTTACCTTTCGCCTGAAACAA ACCCAGCATCATCAACCTCTGCTGTTAATCATAATGTAAATTTAACTAATGTCAGACTGGAGGAGCCCAC CCCAGCTCCTTCCACCTCTTACTCACCACAAGCTGATTCTTTAAGAACACCAAGTACTGAGGCAGCTCAC ATAATGCTAAGAGATCAAGAACCATCATTGTCGTCGCATGTAGATCCAACAAAGGTGTATAGAAAGGATC CATATCCCGAGGAAATGATGAGGCAGAACCATGTTTTGAAACAGCCAGCCGTTAGTCACCCAGGGCACAG GCCAGACAAAGAGCCTAATCTGACCTATGAACCCCAACTCCCATACGTAGAGAAACAAGCCAGCAGAGAC CTCGAGCAGCCCACATACAGATACGAGTCCTCAAGCTATACGGACCAGTTTTCTCGAAACTATGAACATC GTCTGCGATACGAAGATCGCGTCCCCATGTATGAAGAACAGTGGTCATATTATGATGACAAACAGCCCTA CCCATCTCGGCCACCTTTTGATAATCAGCACTCTCAAGACCTTGACTCCAGACAGCATCCCGAAGAGTCC TCAGAACGAGGGTACTTTCCACGTTTTGAAGAGCCAGCCCCTCTGTCTTACGACAGCAGACCACGTTACG AACAGGCACCTAGAGCATCCGCCCTGCGGCACGAAGAGCAGCCAGCTCCTGGGTATGACACACATGGTAG ACTCAGACCGGAAGCCCAGCCCCACCCTTCAGCAGGGCCCAAGCCTGCAGAGTCCAAGCAGTATTTTGAG CAATATTCACGCAGTTACGAGCAAGTACCACCCCAAGGATTTACCTCTAGAGCAGGTCATTTTGAGCCTC TCCATGGTGCTGCAGCTGTCCCTCCGCTGATACCTTCATCTCAGCATAAGCCAGAAGCTCTGCCTTCAAA CACCAAACCACTGCCTCCACCCCCAACTCAAACCGAAGAAGAGGAAGATCCAGCAATGAAGCCACAGTCT GTACTCACCAGAGTTAAGATGTTTGAAAACAAAAGATCTGCATCCTTAGAGACCAAGAAGGATGTAAATG ACACTGGCAGTTTTAAGCCTCCAGAAGTAGCATCTAAACCTTCAGGTGCTCCCATCATTGGTCCCAAACC CACTTCTCAGAATCAATTCAGTGAACATGACAAAACTCTGTACAGGATCCCAGAACCTCAAAAACCTCAA CTGAAGCCACCTGAAGATATTGTTCGGTCCAATCATTATGACCCTGAAGAAGATGAAGAATATTATCGAA AACAGCTGTCATACTTTGACCGAAGAAGTTTTGAGAATAAGCCTCCTGCACACATTGCCGCCAGCCATCT CTCCGAGCCTGCAAAGCCAGCGCATTCTCAGAATCAATCAAATTTTTCTAGTTATTCTTCAAAGGGAAAG CCTCCTGAAGCTGATGGTGTGGATAGATCATTTGGCGAGAAACGCTATGAACCCATCCAGGCCACTCCCC CTCCTCCTCCATTGCCCTCGCAGTATGCCCAGCCATCTCAGCCTGTCACCAGCGCGTCTCTCCACATACA TTCTAAGGGAGCACATGGTGAAGGTAATTCAGTGTCATTGGATTTTCAGAATTCCTTAGTGTCCAAACCA GACCCACCTCCATCTCAGAATAAGCCAGCAACTTTCAGACCACCAAACCGAGAAGATACTGCTCAGGCAG CTTTCTATCCCCAGAAAAGTTTTCCAGATAAAGCCCCAGTTAATGGAACTGAACAGACTCAGAAAACAGT CACTCCAGCATACAATCGATTCACACCAAAACCATATACAAGTTCTGCCCGACCATTTGAACGCAAGTTT GAAAGTCCTAAATTCAATCACAATCTTCTGCCAAGTGAAACTGCACATAAACCTGACTTGTCTTCAAAAA CTCCCACTTCTCCAAAAACTCTTGTGAAATCGCACAGTTTGGCACAGCCTCCTGAGTTTGACAGTGGAGT TGAAACTTTCTCTATCCATGCAGAGAAGCCTAAATATCAAATAAATAATATCAGCACAGTGCCTAAAGCT ATTCCTGTGAGTCCTTCAGCTGTGGAAGAGGATGAAGATGAAGATGGTCATACTGTGGTGGCCACAGCCC GAGGCATATTTAACAGCAATGGGGGCGTGCTGAGTTCCATAGAAACTGGTGTTAGTATAATTATCCCTCA AGGAGCCATTCCCGAAGGAGTTGAGCAGGAAATCTATTTCAAGGTCTGCCGGGACAACAGCATCCTTCCA CCTTTAGATAAAGAGAAAGGTGAAACACTGCTGAGTCCTTTGGTGATGTGTGGTCCCCATGGCCTCAAGT TCCTGAAGCCTGTGGAGCTGCGCTTACCACACTGTGCGTCCATGACTCCTGACGGTTGGTCTTTTGCTCT AAAATCATCCGACTCCTCGTCGGGTGATCCTAAAACCTGGCAAAACAAGTGTCTTCCCGGAGATCCAAAT TATCTCGTTGGAGCAAACTGTGTTTCTGTCCTTATTGACCACTTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC240215 representing NM_001301025
Red=Cloning site Green=Tags(s) MKYQKYLTVLQMAIGVTPSNRGSLLPLKRKLWVTPSSENPNGATSSVSQGKPSLRRIKGRLHRSKSLDSM DFCELTSTAMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQEND RVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSRPDPEPVSDNEEDSYDEEIHDPRSGR SGVVNRRSEKIWPRDRSASRERSLSPRSDRRSVASSQPAKPTKVTLVKSRKNEEYGLRLASHIFVKEISQ DSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANA SERDDISEIQSLASDHSGRSHDRPPRRSRSRSPDQRSEPSDHSRHSPQQPSNGSLRSRDEERISKPGAVS TPVKHADDHTPKTVEEVTVERNEKQTPSLPEPKPVYAQVGQPDVDLPVSPSDGVLPNSTHEDGILRPSMK LVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKG EEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIG KNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAY ERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDQRSSGIIRLHTIKQIIDQDKH ALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTI NLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDY EDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPPYSPQAQPQPIHRIDSP GFKPASQQKAEASSPVPYLSPETNPASSTSAVNHNVNLTNVRLEEPTPAPSTSYSPQADSLRTPSTEAAH IMLRDQEPSLSSHVDPTKVYRKDPYPEEMMRQNHVLKQPAVSHPGHRPDKEPNLTYEPQLPYVEKQASRD LEQPTYRYESSSYTDQFSRNYEHRLRYEDRVPMYEEQWSYYDDKQPYPSRPPFDNQHSQDLDSRQHPEES SERGYFPRFEEPAPLSYDSRPRYEQAPRASALRHEEQPAPGYDTHGRLRPEAQPHPSAGPKPAESKQYFE QYSRSYEQVPPQGFTSRAGHFEPLHGAAAVPPLIPSSQHKPEALPSNTKPLPPPPTQTEEEEDPAMKPQS VLTRVKMFENKRSASLETKKDVNDTGSFKPPEVASKPSGAPIIGPKPTSQNQFSEHDKTLYRIPEPQKPQ LKPPEDIVRSNHYDPEEDEEYYRKQLSYFDRRSFENKPPAHIAASHLSEPAKPAHSQNQSNFSSYSSKGK PPEADGVDRSFGEKRYEPIQATPPPPPLPSQYAQPSQPVTSASLHIHSKGAHGEGNSVSLDFQNSLVSKP DPPPSQNKPATFRPPNREDTAQAAFYPQKSFPDKAPVNGTEQTQKTVTPAYNRFTPKPYTSSARPFERKF ESPKFNHNLLPSETAHKPDLSSKTPTSPKTLVKSHSLAQPPEFDSGVETFSIHAEKPKYQINNISTVPKA IPVSPSAVEEDEDEDGHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPEGVEQEIYFKVCRDNSILP PLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGWSFALKSSDSSSGDPKTWQNKCLPGDPN YLVGANCVSVLIDHF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001301025 |
| ORF Size | 5505 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001301025.1, NP_001287954.1 |
| RefSeq Size | 7795 bp |
| RefSeq ORF | 5586 bp |
| Locus ID | 7082 |
| Cytogenetics | 15q13.1 |
| Protein Families | Druggable Genome |
| Protein Pathways | Adherens junction, Epithelial cell signaling in Helicobacter pylori infection, Gap junction, Tight junction, Vibrio cholerae infection |
| MW | 205.5 kDa |
| Gene Summary | This gene encodes a member of the membrane-associated guanylate kinase (MAGUK) family of proteins, and acts as a tight junction adaptor protein that also regulates adherens junctions. Tight junctions regulate the movement of ions and macromolecules between endothelial and epithelial cells. The multidomain structure of this scaffold protein, including a postsynaptic density 95/disc-large/zona occludens (PDZ) domain, a Src homology (SH3) domain, a guanylate kinase (GuK) domain and unique (U) motifs all help to co-ordinate binding of transmembrane proteins, cytosolic proteins, and F-actin, which are required for tight junction function. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2017] |
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