CTNNA2 (NM_001282598) Human Tagged ORF Clone

CAT#: RC239805

CTNNA2 (myc-DDK-tagged) - Human catenin (cadherin-associated protein), alpha 2 (CTNNA2), transcript variant 4

Product Images

Circular map for RC239805

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CTNNA2
• Synonyms: CAP-R; CAPR; CDCBM9; CT114; CTNR
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC239805 representing NM_001282598
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTGGGTTGATCTGCCTTCAATATGGGCTGTGGCATGGGCAGAAGATTGATTTTGGGGGCCCCAGGA
  GACTGCTTCAAAGAAATAGAGGTGAAGGGAGCATGACTTCGGCAACTTCACCTATCATTCTGAAATGGGA
  CCCCAAAAGTTTGGAAATCCGGACGCTAACAGTGGAAAGGCTGTTGGAGCCACTTGTTACACAGGTGACT
  ACACTTGTCAACACAAGCAACAAAGGCCCATCTGGTAAAAAGAAAGGGAGGTCAAAGAAAGCCCATGTAC
  TAGCTGCCTCTGTAGAGCAAGCCACTCAGAATTTCCTGGAAAAGGGTGAACAGATCGCTAAGGAGAGTCA
  AGATCTCAAAGAAGAGTTGGTGGCTGCTGTAGAGGATGTGCGCAAACAAGGTGAGACGATGCGGATCGCC
  TCCTCCGAGTTTGCAGATGACCCTTGCTCGTCGGTAAAGCGCGGCACCATGGTACGGGCGGCAAGGGCTT
  TGCTCTCCGCGGTGACACGCTTACTCATCCTGGCGGACATGGCAGATGTCATGAGACTTTTATCCCATCT
  GAAAATTGTGGAAGAGGCCCTGGAAGCTGTCAAAAATGCTACAAATGAGCAAGACCTTGCAAACCGTTTT
  AAAGAGTTTGGGAAAGAGATGGTGAAACTTAACTATGTAGCAGCAAGAAGACAACAGGAGCTGAAGGATC
  CTCACTGTCGGGATGAGATGGCAGCCGCCCGAGGGGCTCTGAAGAAGAATGCCACAATGCTGTACACGGC
  CTCTCAAGCATTTCTCCGCCACCCAGATGTCGCCGCTACGAGAGCCAACCGAGATTATGTGTTCAAACAA
  GTCCAGGAGGCCATCGCCGGCATCTCCAATGCTGCTCAAGCTACCTCGCCCACTGACGAAGCCAAGGGCC
  ACACGGGCATCGGCGAGCTGGCTGCGGCTCTTAATGAGTTTGACAATAAGATTATCCTGGACCCCATGAC
  GTTCAGCGAGGCCAGGTTCCGGCCGTCCCTGGAGGAGAGGCTGGAGAGCATCATCAGCGGCGCAGCGCTG
  ATGGCCGACTCCTCCTGCACGCGAGACGACCGGCGCGAGAGGATCGTGGCGGAGTGCAACGCCGTGCGGC
  AGGCGCTCCAGGACCTGCTCAGCGAGTACATGAATAATACTGGAAGGAAAGAAAAAGGAGATCCTCTCAA
  CATTGCGATTGATAAGATGACTAAGAAAACAAGAGATCTAAGGAGACAGCTTCGGAAAGCAGTGATGGAT
  CACATATCTGACTCTTTCCTGGAAACCAATGTTCCTTTGCTAGTTCTCATTGAGGCTGCAAAGAGCGGAA
  ATGAAAAGGAAGTGAAAGAATATGCCCAAGTTTTCCGTGAGCATGCCAACAAACTGGTAGAGGTTGCCAA
  TTTGGCCTGTTCCATCTCCAACAATGAAGAAGGGGTGAAATTAGTTCGGATGGCAGCCACCCAGATTGAC
  AGCCTGTGTCCCCAGGTCATCAATGCCGCTCTGACACTGGCTGCCCGGCCACAGAGCAAAGTTGCTCAGG
  ATAACATGGACGTCTTCAAAGACCAGTGGGAGAAGCAGGTCCGAGTGTTGACAGAGGCCGTGGATGACAT
  CACCTCAGTGGATGACTTCCTCTCTGTCTCAGAAAATCACATCTTGGAGGATGTGAACAAGTGTGTGATA
  GCCCTCCAAGAGGGCGATGTGGACACTCTGGACCGGACTGCAGGGGCCATCAGGGGCCGGGCAGCTCGAG
  TCATACACATCATCAATGCTGAGATGGAGAACTATGAAGCTGGGGTTTATACTGAGAAGGTGTTGGAAGC
  TACAAAATTGCTTTCTGAAACAGTGATGCCACGCTTCGCTGAACAAGTAGAGGTTGCCATTGAAGCCCTG
  AGTGCCAACGTTCCTCAACCGTTTGAGGAGAATGAGTTCATCGATGCCTCTCGCCTGGTGTATGATGGCG
  TTCGGGACATCAGAAAGGCTGTGCTGATGATCAGGACCCCAGAAGAACTAGAGGATGATTCTGACTTTGA
  GCAGGAAGATTATGATGTGCGTAGCAGGACAAGTGTTCAGACTGAGGATGACCAGCTCATTGCAGGGCAG
  AGCGCACGGGCCATCATGGCGCAACTACCGCAGGAGGAGAAGGCAAAAATAGCTGAGCAGGTGGAGATAT
  TCCATCAAGAGAAAAGCAAGCTGGATGCAGAAGTGGCCAAATGGGACGACAGCGGCAATGATATCATTGT
  ACTGGCCAAGCAGATGTGTATGATCATGATGGAAATGACAGACTTCACAAGAGGCAAAGGCCCATTGAAA
  AATACATCTGATGTCATTAATGCTGCCAAGAAAATTGCCGAAGCAGGTTCTCGAATGGACAAATTAGCTC
  GTGCTGTGGCTGATCAGTGTCCTGATTCAGCATGTAAGCAGGATTTATTAGCCTACCTTCAACGAATTGC
  CTTGTATTGCCATCAGCTTAATATCTGCAGCAAGGTGAAGGCAGAAGTGCAGAATCTGGGAGGAGAGCTC
  ATTGTGTCAGGGCTGGACAGTGCCACATCGCTTATCCAGGCAGCTAAAAACCTGATGAATGCTGTTGTCC
  TCACGGTGAAAGCATCCTATGTGGCCTCAACCAAATACCAGAAGGTCTATGGGACAGCAGCTGTCAACTC
  ACCTGTTGTGTCTTGGAAGATGAAGGCTCCAGAGAAGAAGCCCCTTGTGAAGAGAGAAAAGCCTGAAGAA
  TTCCAGACACGAGTTCGACGAGGTTCTCAGAAGAAACACATTTCGCCTGTACAGGCTTTAAGTGAATTCA
  AAGCAATGGATTCCTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC239805 representing NM_001282598
  Red=Cloning site Green=Tags(s)
  
  MSGLICLQYGLWHGQKIDFGGPRRLLQRNRGEGSMTSATSPIILKWDPKSLEIRTLTVERLLEPLVTQVT
  TLVNTSNKGPSGKKKGRSKKAHVLAASVEQATQNFLEKGEQIAKESQDLKEELVAAVEDVRKQGETMRIA
  SSEFADDPCSSVKRGTMVRAARALLSAVTRLLILADMADVMRLLSHLKIVEEALEAVKNATNEQDLANRF
  KEFGKEMVKLNYVAARRQQELKDPHCRDEMAAARGALKKNATMLYTASQAFLRHPDVAATRANRDYVFKQ
  VQEAIAGISNAAQATSPTDEAKGHTGIGELAAALNEFDNKIILDPMTFSEARFRPSLEERLESIISGAAL
  MADSSCTRDDRRERIVAECNAVRQALQDLLSEYMNNTGRKEKGDPLNIAIDKMTKKTRDLRRQLRKAVMD
  HISDSFLETNVPLLVLIEAAKSGNEKEVKEYAQVFREHANKLVEVANLACSISNNEEGVKLVRMAATQID
  SLCPQVINAALTLAARPQSKVAQDNMDVFKDQWEKQVRVLTEAVDDITSVDDFLSVSENHILEDVNKCVI
  ALQEGDVDTLDRTAGAIRGRAARVIHIINAEMENYEAGVYTEKVLEATKLLSETVMPRFAEQVEVAIEAL
  SANVPQPFEENEFIDASRLVYDGVRDIRKAVLMIRTPEELEDDSDFEQEDYDVRSRTSVQTEDDQLIAGQ
  SARAIMAQLPQEEKAKIAEQVEIFHQEKSKLDAEVAKWDDSGNDIIVLAKQMCMIMMEMTDFTRGKGPLK
  NTSDVINAAKKIAEAGSRMDKLARAVADQCPDSACKQDLLAYLQRIALYCHQLNICSKVKAEVQNLGGEL
  IVSGLDSATSLIQAAKNLMNAVVLTVKASYVASTKYQKVYGTAAVNSPVVSWKMKAPEKKPLVKREKPEE
  FQTRVRRGSQKKHISPVQALSEFKAMDSF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001282598
• ORF Size: 2817 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001282598.1, NP_001269527.1
• RefSeq Size: 3858 bp
• RefSeq ORF: 2820 bp
• Locus ID: 1496
• UniProt ID: P26232
• Cytogenetics: 2p12
• Protein Families: Druggable Genome
• Protein Pathways: Adherens junction, Arrhythmogenic right ventricular cardiomyopathy (ARVC), Endometrial cancer, Leukocyte transendothelial migration, Pathways in cancer, Tight junction
• MW: 104.7 kDa
• Gene Summary: May function as a linker between cadherin adhesion receptors and the cytoskeleton to regulate cell-cell adhesion and differentiation in the nervous system (By similarity). Required for proper regulation of cortical neuronal migration and neurite growth (PubMed:30013181). It acts as negative regulator of Arp2/3 complex activity and Arp2/3-mediated actin polymerization (PubMed:30013181). It thereby suppresses excessive actin branching which would impair neurite growth and stability (PubMed:30013181). Regulates morphological plasticity of synapses and cerebellar and hippocampal lamination during development. Functions in the control of startle modulation (By similarity).[UniProtKB/Swiss-Prot Function]