ZNF480 (NM_001297625) Human Tagged ORF Clone

CAT#: RC238343

ZNF480 (myc-DDK-tagged) - Human zinc finger protein 480 (ZNF480), transcript variant 3

Product Images

Circular map for RC238343

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ZNF480
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC238343 representing NM_001297625
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTGGAGCAAAGGAGGGAGCCCTGGTCTGGTGAGAGTGAAGTGAAAATAGCAAAAAATTCAGATGGGA
  GGGAGTGCATCAAAGGTGTGAACACAGGGAGCAGCTATGCATTGGGAAGCAATGCAGAAGACAAACCAAT
  TAAAAAACAACTTGGAGTATCCTTTCACTTACATCTGTCTGAACTGGAGCTATTTCCAGATGAAAGGGTA
  ATAAATGGATGTAATCAAGTTGAAAACTTTATCAACCACAGTTCCTCTGTTTCCTGTCTTCAAGAAATGT
  CTTCCAGTGTCAAAACCCCCATTTTTAATAGGAATGATTTTGATGATTCTTCATTTCTCCCACAAGAACA
  GAAAGTACACCTTAGAGAAAAACCTTATGAATGTAATGAGCATAGCAAAGTCTTTAGAGTATCTTCCAGC
  CTTACTAAACATCAAGTAATCCATACTGTAGAGAAACCTTACAAATGTAATTCATGCGGCAAGGTCTTTA
  GTCGCAATTCACACCTTGCAGAACATTGTAGAATTCATACTGGAGAGAAACCTTACAAATGTAATGTCTG
  TGGCAAGGTTTTTAGTTACAATTCAAACTTTGCACGACATCAAAGAATTCATACCAGAGAGAAGCCGTAT
  GAATGTAATGAATGTGGTAAAGTCTTCAGTAATAATTCTTACCTTGCACGACATCAAAGAATTCATGCTG
  AAGAGAAACCTTACAAATGTAATGAATGTGGTAAAGGCTTCAGTCATAAGTCATCTCTAGCAAATCATTG
  GAGAATTTATACTGGAGAGAAGCCTTACAAATGTGATGAATGTGGCAAGGCCTTCTATAGGATTGCGCTC
  CTTGTACGACATCAGAAAATTCATACTGGAGAGAAACCTTACAAATGTAATGAATGTGGAAAGGTCTTTA
  TTCAAAATTCGCACCTAGCACAACATTGGAGAATTCATACAGGAGAGAAACCTTACAAATGTAATGAATG
  TGGAAAAGTATTTAATCAACTTTCAAATCTTGCACGACATCGAAGAATTCATACTGGAGAGAAGCCTTAC
  AAATGTAATGAATGTGGTAAAGCATTTAGTGAGTATTCAGGCCTTTCAGCCCATCTTGTAATCCACACTG
  GAGAGAAGCCTTACAAATGTAGTGAATGTGGCAAGGCATTCAGACACAAGTTATCACTAACCAATCATCA
  GAGAATCCATACTGGAGAAAGACCTTACAAATGTAATGAATGTGGCAAGGTCTTCAATCGAATTGCACAC
  CTTGCACGACATCGGAAAATTCATACTGGAGAGAAACCTTACAAATGTAATGAGTGTGGCAAGGCCTTTA
  GTCGCATTTCATACCTAGCACAACATTGGACAATTCATATGGGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC238343 representing NM_001297625
  Red=Cloning site Green=Tags(s)
  
  MLEQRREPWSGESEVKIAKNSDGRECIKGVNTGSSYALGSNAEDKPIKKQLGVSFHLHLSELELFPDERV
  INGCNQVENFINHSSSVSCLQEMSSSVKTPIFNRNDFDDSSFLPQEQKVHLREKPYECNEHSKVFRVSSS
  LTKHQVIHTVEKPYKCNSCGKVFSRNSHLAEHCRIHTGEKPYKCNVCGKVFSYNSNFARHQRIHTREKPY
  ECNECGKVFSNNSYLARHQRIHAEEKPYKCNECGKGFSHKSSLANHWRIYTGEKPYKCDECGKAFYRIAL
  LVRHQKIHTGEKPYKCNECGKVFIQNSHLAQHWRIHTGEKPYKCNECGKVFNQLSNLARHRRIHTGEKPY
  KCNECGKAFSEYSGLSAHLVIHTGEKPYKCSECGKAFRHKLSLTNHQRIHTGERPYKCNECGKVFNRIAH
  LARHRKIHTGEKPYKCNECGKAFSRISYLAQHWTIHMG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001297625
• ORF Size: 1374 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001297625.1, NP_001284554.1
• RefSeq Size: 4640 bp
• RefSeq ORF: 1377 bp
• Locus ID: 147657
• UniProt ID: Q8WV37
• Cytogenetics: 19q13.41
• Protein Families: Transcription Factors
• MW: 53.3 kDa
• Gene Summary: Involved in transcriptional regulation as an activator.[UniProtKB/Swiss-Prot Function]