RALGDS (NM_001271776) Human Tagged ORF Clone

CAT#: RC235009

RALGDS (Myc-DDK tagged) - Homo sapiens ral guanine nucleotide dissociation stimulator (RALGDS), transcript variant 5

Product Images

Circular map for RC235009

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: RALGDS
• Synonyms: RalGEF; RGDS; RGF
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC235009 representing NM_001271776
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGCAGCGCATGTGGGCCGAGGCGGCCGGGCCTGCTGGCGGCGCCGAGCCGCTGTTTCCGGGCTCCC
  GGCGGAGCCGCAGCGTGTGGGACGCCGTGCGCCTGGAGGTGGGCGTCCCCGACAGCTGCCCGGTGGTGCT
  GCACAGCTTCACGCAGCTAGACCCCGACCTGCCGCGCCCGGAGAGCTCCACGCAGGAGATCGGTGAGGAG
  CTGATCAACGGAGTCATCTACTCCATCTCCCTGCGCAAGGTGCAGCTGCACCACGGAGGCAACAAGGGGC
  AGCGCTGGCTCGGGTATGAGAATGAGTCGGCCCTGAACCTTTATGAGACTTGCAAGGTGCGGACCGTGAA
  GGCTGGCACGCTGGAGAAGCTGGTGGAGCACCTGGTGCCAGCCTTCCAGGGCAGCGACCTCTCCTACGTC
  ACCATCTTCCTGTGTACCTATAGAGCCTTCACCACCACCCAACAGGTCCTGGACCTGCTGTTCAAAAGAT
  ACGGCTGCATCCTCCCCTATTCCGACGAGGATGGTGGACCCCAGGACCAACTTAAAAATGCCATCTCCTC
  CATCCTGGGCACCTGGCTGGACCAGTACTCGGAGGATTTCTGTCAACCTCCGGACTTTCCCTGCCTCAAG
  CAGCTGGTGGCCTACGTGCAGCTCAACATGCCAGGCTCAGACCTGGAGCGCCGTGCCCACCTTCTCCTGG
  CCCAGCTGGAGCACTCGGAACCCATTGAGGCAGAGCCTGAGGCTCTGTCACCAGTGCCAGCTCTAAAACC
  AACTCCAGAGCTCGAGCTAGCTCTAACACCAGCTCGAGCACCCAGCCCAGTGCCGGCTCCAGCCCCGGAG
  CCAGAGCCAGCTCCAACACCAGCTCCAGGTTCAGAGCTAGAAGTAGCTCCAGCACCAGCTCCGGAGCTCC
  AGCAGGCTCCAGAGCCAGCTGTGGGACTAGAATCGGCTCCAGCGCCAGCTCTGGAACTAGAGCCAGCTCC
  AGAACAGGATCCAGCTCCCTCACAAACTCTAGAGCTGGAGCCAGCTCCAGCACCAGTTCCATCATTACAG
  CCTTCCTGGCCTTCACCTGTGGTTGCAGAGAACGGGCTGAGTGAGGAGAAGCCTCACCTCTTGGTGTTCC
  CTCCAGATCTGGTGGCAGAGCAGTTTACACTGATGGATGCGGAACTGTTCAAGAAGGTGGTGCCCTACCA
  CTGCCTGGGCTCCATCTGGTCCCAGCGGGACAAGAAGGGCAAGGAGCACCTGGCGCCCACCATCCGCGCC
  ACTGTCACCCAGTTCAACAGTGTGGCCAACTGTGTCATCACCACCTGCCTCGGGAACCGAAGCACGAAAG
  CCCCAGACAGGGCCAGGGTGGTGGAGCACTGGATCGAGGTGGCCAGGGAGTGCCGGATCCTCAAGAACTT
  CTCGTCACTGTATGCCATCCTCTCTGCCCTGCAGAGCAACTCCATCCACCGTCTGAAGAAGACGTGGGAA
  GACGTTTCCAGGGACAGTTTCCGGATCTTTCAGAAGCTGTCAGAGATCTTCTCAGATGAGAACAACTACT
  CATTGAGCCGGGAGCTGCTCATCAAGGAGGGCACCTCCAAGTTTGCCACCCTGGAGATGAACCCCAAGAG
  AGCCCAGAAACGGCCGAAGGAGACGGGCATCATCCAGGGCACCGTTCCCTACCTGGGCACGTTCCTCACC
  GACCTGGTGATGCTGGACACTGCCATGAAGGACTATCTGTATGGCAGACTCATCAACTTTGAGAAGAGGA
  GGAAGGAGTTCGAGGTGATCGCCCAGATCAAGCTGCTGCAGTCGGCCTGCAACAACTACAGCATCGCGCC
  AGATGAGCAATTTGGGGCCTGGTTCCGGGCCGTGGAGCGGCTCAGCGAGACTGAGAGCTACAACCTGTCG
  TGCGAGCTGGAGCCCCCATCCGAGTCAGCCAGCAACACCCTCAGGACCAAGAAGAACACAGCCATTGTCA
  AGCGCTGGAGCGACCGCCAGGCCCCCAGCACTGAGCTCAGTACCAGTGGCAGCTCCCACTCCAAGTCCTG
  TGACCAGCTCAGGTGTGGCCCCTACCTCAGCAGCGGGGACATCGCTGACGCGCTCAGCGTGCACTCGGCC
  GGCTCCTCTAGCTCCGACGTGGAGGAGATCAACATCAGCTTCGTCCCGGAGTCTCCTGATGGCCAGGAAA
  AGAAGTTCTGGGAATCAGCCTCACAGTCATCCCCGGAGACCTCCGGCATCAGCTCAGCCTCCAGCAGCAC
  CTCGTCCTCCTCAGCCTCCACCACGCCCGTGGCTGCCACACGCACCCACAAGCGCTCTGTCTCAGGGCTC
  TGCAACTCCAGCTCCGCGCTGCCGCTCTACAACCAGCAGGTGGGCGACTGCTGTATCATCCGCGTCAGCC
  TGGACGTGGACAATGGCAACATGTACAAGAGCATCCTGGTGACCAGCCAAGATAAGGCTCCGGCTGTAAT
  CCGCAAGGCCATGGACAAACACAACCTGGAGGAGGAGGAGCCGGAGGACTATGAGCTGCTGCAGATTCTC
  TCAGATGACCGGAAGCTGAAGATCCCTGAAAACGCCAACGTCTTCTATGCCATGAACTCTACCGCCAACT
  ATGACTTTGTCCTCAAGAAGCGGACCTTCACCAAGGGAGTGAAGGTCAAGCACGGAGCCAGCTCCACCCT
  CCCTCGCATGAAGCAGAAAGGACTCAAGATTGCCAAGGGCATCTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC235009 representing NM_001271776
  Red=Cloning site Green=Tags(s)
  
  MVQRMWAEAAGPAGGAEPLFPGSRRSRSVWDAVRLEVGVPDSCPVVLHSFTQLDPDLPRPESSTQEIGEE
  LINGVIYSISLRKVQLHHGGNKGQRWLGYENESALNLYETCKVRTVKAGTLEKLVEHLVPAFQGSDLSYV
  TIFLCTYRAFTTTQQVLDLLFKRYGCILPYSDEDGGPQDQLKNAISSILGTWLDQYSEDFCQPPDFPCLK
  QLVAYVQLNMPGSDLERRAHLLLAQLEHSEPIEAEPEALSPVPALKPTPELELALTPARAPSPVPAPAPE
  PEPAPTPAPGSELEVAPAPAPELQQAPEPAVGLESAPAPALELEPAPEQDPAPSQTLELEPAPAPVPSLQ
  PSWPSPVVAENGLSEEKPHLLVFPPDLVAEQFTLMDAELFKKVVPYHCLGSIWSQRDKKGKEHLAPTIRA
  TVTQFNSVANCVITTCLGNRSTKAPDRARVVEHWIEVARECRILKNFSSLYAILSALQSNSIHRLKKTWE
  DVSRDSFRIFQKLSEIFSDENNYSLSRELLIKEGTSKFATLEMNPKRAQKRPKETGIIQGTVPYLGTFLT
  DLVMLDTAMKDYLYGRLINFEKRRKEFEVIAQIKLLQSACNNYSIAPDEQFGAWFRAVERLSETESYNLS
  CELEPPSESASNTLRTKKNTAIVKRWSDRQAPSTELSTSGSSHSKSCDQLRCGPYLSSGDIADALSVHSA
  GSSSSDVEEINISFVPESPDGQEKKFWESASQSSPETSGISSASSSTSSSSASTTPVAATRTHKRSVSGL
  CNSSSALPLYNQQVGDCCIIRVSLDVDNGNMYKSILVTSQDKAPAVIRKAMDKHNLEEEEPEDYELLQIL
  SDDRKLKIPENANVFYAMNSTANYDFVLKKRTFTKGVKVKHGASSTLPRMKQKGLKIAKGIF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001271776
• ORF Size: 2706 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001271776.2
• RefSeq Size: 3694 bp
• RefSeq ORF: 2709 bp
• Locus ID: 5900
• UniProt ID: Q12967
• Cytogenetics: 9q34.13-q34.2
• Protein Pathways: Colorectal cancer, Pancreatic cancer, Pathways in cancer
• MW: 99.8 kDa
• Gene Summary: Guanine nucleotide dissociation stimulators (GDSs, or exchange factors), such as RALGDS, are effectors of Ras-related GTPases (see MIM 190020) that participate in signaling for a variety of cellular processes.[supplied by OMIM, Nov 2010]