STK23 (SRPK3) (NM_001170760) Human Tagged ORF Clone

CAT#: RC230283

SRPK3 (Myc-DDK-tagged)-Human SRSF protein kinase 3 (SRPK3), transcript variant 2

Product Images

Circular map for RC230283

• 

  Western blot validation of overexpression lysate (Cat# LY433283) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC230283 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: STK23
• Synonyms: MSSK-1; MSSK1; STK23
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC230283 representing NM_001170760
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCGCCAGCACGGGCGGTGGTGGGGACAGCGGCGGCAGCGGCGGCAGTAGCAGCAGCTCACAGGCCT
  CCTGCGGGCCCGAGTCCTCGGGCTCCGAACTAGCCCTGGCCACACCGGTGCCTCAGATGCTGCAGGGCCT
  TCTGGGCTCCGACGACGAGGAACAGGAAGACCCCAAAGACTACTGCAAGGGCGGCTACCACCCTGTGAAG
  ATCGGCGACGTGTTCAATGGGCGGTACCACGTGGTGCGCAAACTGGGCTGGGGCCACTTCTCCACCGTCT
  GGCTCTGCTGGGACATCCAGCGCAAGCGCTTTGTGGCCCTCAAAGTGGTGAAGAGTGCGGGGCATTACAC
  GGAGACAGCTGTGGATGAGATCAAGCTCCTGAAATGTGTCCGGGACAGCGACCCCAGTGACCCCAAAAGA
  GAGACCATTGTCCAGCTCATTGATGACTTCAGGATCTCAGGAGTCAATGGAGTCCATGTGTGCATGGTGC
  TGGAGGTGCTGGGCCACCAGCTCCTCAAATGGATCATCAAGTCCAACTACCAGGGCCTGCCCGTGCCCTG
  CGTGAAGAGCATCGTGAGGCAGGTGCTGCACGGCCTGGACTACCTCCACACCAAGTGCAAGATCATCCAC
  ACGGACATCAAGCCCGAGAACATCTTGCTGTGTGTGGGGGACGCTTACATCAGGCGCCTGGCTGCCGAGG
  CCACGGAGTGGCAACAGGCAGGGGCGCCGCCCCCCTCCCGCTCCATAGTCAGCACTGCCCCCCAGGAGGT
  CTTGACCGGTAAGCTGTCCAAAAACAAGAGGAAGAAGATGAGGCGCAAACGGAAACAGCAGAAGCGGCTG
  CTGGAGGAGCGGCTGCGGGACCTGCAGAGGCTGGAGGCCATGGAGGCTGCCACCCAGGCTGAGGACTCTG
  GCTTGAGACTAGACGGGGGCAGCGGCTCCACATCCTCTTCAGGCTGTCACCCCGGGGGCGCCAGAGCAGG
  TCCCTCCCCAGCCTCTTCCTCCCCCGCCCCAGGGGGCGGCCGTAGCCTCAGCGCGGGCTCACAGACCTCA
  GGCTTCTCCGGCTCCCTCTTCTCTCCTGCCTCCTGCTCCATCCTCTCCGGCTCGTCCAATCAGCGAGAGA
  CCGGGGGCCTCCTGTCGCCTAGCACACCATTCGGTGCCTCGAACCTCCTGGTGAACCCCCTGGAGCCCCA
  AAATGCAGATAAGATCAAGATCAAGATCGCAGACCTGGGCAACGCCTGCTGGGTGCACAAGCACTTCACG
  GAAGACATCCAGACTCGGCAGTACCGGGCCGTCGAGGTGCTGATCGGCGCCGAATACGGCCCCCCGGCAG
  ACATCTGGAGCACAGCCTGCATGGCCTTCGAGCTGGCCACTGGTGACTACCTGTTCGAGCCGCATTCTGG
  AGAAGACTACAGTCGTGATGAGGACCACATCGCTCACATAGTGGAGCTTCTGGGGGACATCCCCCCAGCC
  TTCGCCCTCTCAGGCCGCTATTCCCGGGAGTTCTTCAACCGGAGAGGAGAGCTGCGGCACATCCACAATC
  TCAAGCACTGGGGCCTGTACGAGGTACTCATGGAAAAGTACGAGTGGCCCCTAGAGCAGGCCACACAGTT
  CAGCGCCTTTCTGCTGCCCATGATGGAGTACATCCCCGAAAAGCGGGCCAGTGCCGCTGACTGCCTCCAG
  CACCCCTGGCTCAACCCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC230283 representing NM_001170760
  Red=Cloning site Green=Tags(s)
  
  MSASTGGGGDSGGSGGSSSSSQASCGPESSGSELALATPVPQMLQGLLGSDDEEQEDPKDYCKGGYHPVK
  IGDVFNGRYHVVRKLGWGHFSTVWLCWDIQRKRFVALKVVKSAGHYTETAVDEIKLLKCVRDSDPSDPKR
  ETIVQLIDDFRISGVNGVHVCMVLEVLGHQLLKWIIKSNYQGLPVPCVKSIVRQVLHGLDYLHTKCKIIH
  TDIKPENILLCVGDAYIRRLAAEATEWQQAGAPPPSRSIVSTAPQEVLTGKLSKNKRKKMRRKRKQQKRL
  LEERLRDLQRLEAMEAATQAEDSGLRLDGGSGSTSSSGCHPGGARAGPSPASSSPAPGGGRSLSAGSQTS
  GFSGSLFSPASCSILSGSSNQRETGGLLSPSTPFGASNLLVNPLEPQNADKIKIKIADLGNACWVHKHFT
  EDIQTRQYRAVEVLIGAEYGPPADIWSTACMAFELATGDYLFEPHSGEDYSRDEDHIAHIVELLGDIPPA
  FALSGRYSREFFNRRGELRHIHNLKHWGLYEVLMEKYEWPLEQATQFSAFLLPMMEYIPEKRASAADCLQ
  HPWLNP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001170760
• ORF Size: 1698 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001170760.2
• RefSeq ORF: 1701 bp
• Locus ID: 26576
• UniProt ID: Q9UPE1
• Cytogenetics: Xq28
• Protein Families: Druggable Genome, Protein Kinase
• MW: 62.3 kDa
• Gene Summary: This gene encodes a protein kinase similar to a protein kinase which is specific for the SR (serine/arginine-rich domain) family of splicing factors. A highly similar protein has been shown to play a role in muscle development in mice. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2009]