BAT5 (ABHD16A) (NM_001177515) Human Tagged ORF Clone

CAT#: RC230230

ABHD16A (Myc-DDK-tagged)-Human abhydrolase domain containing 16A (ABHD16A), transcript variant 2

Product Images

Circular map for RC230230

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: BAT5
• Synonyms: BAT5; D6S82E; hBAT5; NG26; PP199
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC230230 representing NM_001177515
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCTCCCCCGGCTCTGTTTTTATCTTCCCTTTACCCTCGCCTTGAATTTCAGAATGACTTTTACAGAT
  CCTGTATCCGTCGGTCCTCCCCTCAACCCCCGCCCAACCTAGCCTGGAGACCCGAGTCATTGTATTCTGG
  AGAGCTGGCGGGTGGTGGTTACTTGAGTTTGTCCAAAGTGGTGCCGTTTTCTCACTATGCTGGGACATTG
  CTGCTACTTCTGGCAGGTGTGGCCTGCCTCCGAGGCATTGGCCGCTGGACCAACCCCCAGTACCGGCAGT
  TCATCACCATCTTGGAAGCAACACATCGGAACCAGTCTTCAGAAAACAAGAGGCAGCTTGCCAACTACAA
  CTTTGACTTCCGGAGCTGGCCAGTCGACTTCCACTGGGAAGAACCCAGCAGCCGGAAGGAGTCTCGAGGG
  GGCCCTTCCCGCCGGGGTGTGGCCCTGCTTCGCCCAGAGCCCCTGCACCGGGGGACAGCAGACACCCTCC
  TCAACCGGGTTAAGAAGCTGCCTTGTCAGATCACCAGCTACCTGGTGGCGCACACCCTAGGGCGCCGGAT
  GCTGTATCCAGGCTCTGTGTACCTGCTGCAGAAGGCCCTCATGCCTGTGCTGCTGCAGGGCCAGGCCCGA
  CTGGTGGAAGAGTGTAATGGGCGCCGGGCAAAGCTGCTGGCCTGTGATGGCAATGAGATTGACACCATGT
  TTGTGGACCGGCGGGGGACAGCTGAGCCCCAGGGACAGAAGCTGGTGATCTGCTGTGAGGGGAATGCTGG
  GTTTTATGAGGTGGGCTGCGTCTCCACGCCCCTGGAAGCTGGATATTCAGTCCTGGGCTGGAATCATCCA
  GGCTTTGCTGGAAGCACGGGGGTGCCATTCCCGCAGAATGAGGCTAATGCCATGGATGTGGTGGTCCAGT
  TTGCCATCCACCGCCTAGGCTTCCAGCCCCAGGACATCATCATCTACGCCTGGTCCATCGGCGGCTTCAC
  TGCCACGTGGGCAGCCATGTCCTACCCAGATGTTAGTGCCATGATCCTGGATGCCTCCTTTGATGACCTG
  GTGCCCTTGGCCTTGAAGGTCATGCCAGACAGCTGGAGGGGCCTGGTGACCAGGACCGTGAGGCAGCATC
  TCAATCTAAACAACGCGGAGCAGCTGTGCAGATACCAGGGTCCTGTACTGCTGATCCGGAGAACCAAGGA
  TGAGATCATCACCACCACGGTTCCTGAGGACATCATGTCCAACCGAGGCAATGACCTCCTGCTGAAGCTC
  CTGCAGCATCGGTATCCCCGGGTGATGGCAGAGGAGGGTCTTCGAGTGGTGAGGCAGTGGTTGGAGGCCT
  CCTCACAGCTGGAGGAAGCCTCAATTTATAGCCGATGGGAGGTGGAAGAGGACTGGTGTCTGTCTGTCCT
  CCGCTCCTACCAGGCAGAACACGGGCCCGACTTCCCCTGGAGCGTGGGGGAGGACATGAGTGCAGATGGA
  CGGCGGCAGCTGGCTTTGTTTCTGGCTCGGAAGCATCTGCACAACTTTGAGGCCACTCACTGCACCCCAC
  TCCCAGCCCAGAACTTCCAGATGCCCTGGCACCTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC230230 representing NM_001177515
  Red=Cloning site Green=Tags(s)
  
  MPPPALFLSSLYPRLEFQNDFYRSCIRRSSPQPPPNLAWRPESLYSGELAGGGYLSLSKVVPFSHYAGTL
  LLLLAGVACLRGIGRWTNPQYRQFITILEATHRNQSSENKRQLANYNFDFRSWPVDFHWEEPSSRKESRG
  GPSRRGVALLRPEPLHRGTADTLLNRVKKLPCQITSYLVAHTLGRRMLYPGSVYLLQKALMPVLLQGQAR
  LVEECNGRRAKLLACDGNEIDTMFVDRRGTAEPQGQKLVICCEGNAGFYEVGCVSTPLEAGYSVLGWNHP
  GFAGSTGVPFPQNEANAMDVVVQFAIHRLGFQPQDIIIYAWSIGGFTATWAAMSYPDVSAMILDASFDDL
  VPLALKVMPDSWRGLVTRTVRQHLNLNNAEQLCRYQGPVLLIRRTKDEIITTTVPEDIMSNRGNDLLLKL
  LQHRYPRVMAEEGLRVVRQWLEASSQLEEASIYSRWEVEEDWCLSVLRSYQAEHGPDFPWSVGEDMSADG
  RRQLALFLARKHLHNFEATHCTPLPAQNFQMPWHL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001177515
• ORF Size: 1575 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001177515.2
• RefSeq ORF: 1578 bp
• Locus ID: 7920
• UniProt ID: O95870
• Cytogenetics: 6p21.33
• Protein Families: Transmembrane
• MW: 59.8 kDa
• Gene Summary: A cluster of genes, BAT1-BAT5, has been localized in the vicinity of the genes for tumor necrosis factor alpha and tumor necrosis factor beta. These genes are all within the human major histocompatibility complex class III region. The protein encoded by this gene is thought to be involved in some aspects of immunity. Alternatively spliced transcript variants have been described. [provided by RefSeq, Apr 2010]