ADAM8 (NM_001164490) Human Tagged ORF Clone

CAT#: RC228563

ADAM8 (Myc-DDK-tagged)-Human ADAM metallopeptidase domain 8 (ADAM8), transcript variant 3

Product Images

Circular map for RC228563

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ADAM8
• Synonyms: CD156; CD156a; MS2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC228563 representing NM_001164490
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGCGGCCTCGGGCTCTGGCTGCTGGGCGCGATGATGCTGCCTGGGCCTGCACCCAGAGAGGGTGAGC
  TACGTCCTTGGGGCCACAGGGCACAACTTCACCCTCCACCTGCGGAAGAACAGGGACCTGCTGGGCTCCG
  GCTACACAGAGACCTATACGGCTGCCAATGGCTCCGAGGTGACGGAGCAGCCTCGCGGGCAGGACCACTG
  CTTCTACCAGGGCCACGTAGAGGGGTACCCGGACTCAGCCGCCAGCCTCAGCACCTGTGCCGGCCTCAGG
  TGGGGTCAGACCTGCACCTGATCGAGCCCCTGGATGAAGGTGGCGAGGGCGGACGGCACGCCGTGTACCA
  GGCTGAGCACCTGCTGCAGACGGCCGGGACCTGCGGGGTCAGCGACGACAGCCTGGGCAGCCTCCTGGGA
  CCCCGGACGGCAGCCGTCTTCAGGCCTCGGCCCGGGGACTCTCTGCCATCCCGAGAGACCCGCTACGTGG
  AGCTGTATGTGGTCGTGGACAATGCAGAGTTCCAGATGCTGGGGAGCGAAGCAGCCGTGCGTCATCGGGT
  GCTGGAGGTGGTGAATCACGTGGACAAGCTATATCAGAAACTCAACTTCCGTGTGGTCCTGGTGGGCCTG
  GAGATTTGGAATAGTCAGGACAGGTTCCACGTCAGCCCCGACCCCAGTGTCACACTGGAGAACCTCCTGA
  CCTGGCAGGCACGGCAACGGACACGGCGGCACCTGCATGACAACGTACAGCTCATCACGGGTGTCGACTT
  CACCGGGACTACCGTGGGGTTTGCCAGGGTGTCCGCCATGTGCTCCCACAGCTCAGGGGCTGTGAACCAG
  GACCACAGCAAGAACCCCGTGGGCGTGGCCTGTACCATGGCCCATGAGATGGGCCACAACCTGGGCATGG
  ACCATGATGAGAACGTCCAGGGCTGCCGCTGCCAGGAACGCTTCGAGGCCGGCCGCTGCATCATGGCGGG
  CAGCATTGGCTCCAGTTTCCCCAGGATGTTCAGTGACTGCAGCCAGGCCTACCTGGAGAGCTTTTTGGAG
  CGGCCGCAGTCGGTGTGCCTCGCCAACGCCCCTGACCTCAGCCACCTGGTGGGCGGCCCCGTGTGTGGGA
  ACCTGTTTGTGGAGCGTGGGGAGCAGTGCGACTGCGGCCCCCCCGAGGACTGCCGGAACCGCTGCTGCAA
  CTCTACCACCTGCCAGCTGGCTGAGGGGGCCCAGTGTGCGCACGGTACCTGCTGCCAGGAGTGCAAGGTG
  AAGCCGGCTGGTGAGCTGTGCCGTCCCAAGAAGGACATGTGTGACCTCGAGGAGTTCTGTGACGGCCGGC
  ACCCTGAGTGCCCGGAAGACGCCTTCCAGGAGAACGGCACGCCCTGCTCCGGGGGCTACTGCTACAACGG
  GGCCTGTCCCACACTGGCCCAGCAGTGCCAGGCCTTCTGGGGGCCAGGTGGGCAGGCTGCCGAGGAGTCC
  TGCTTCTCCTATGACATCCTACCAGGCTGCAAGGCCAGCCGGTACAGGGCTGACATGTGTGGCGTTCTGC
  AGTGCAAGGGTGGGCAGCAGCCCCTGGGGCGTGCCATCTGCATCGTGGATGTGTGCCACGCGCTCACCAC
  AGAGGATGGCACTGCGTATGAACCAGTGCCCGAGGGCACCCGGTGTGGACCAGAGAAGGTGTGCAACCAC
  AAGCAGGAGTGCCACTGCCACGCGGGCTGGGCCCCGCCCCACTGCGCGAAGCTGCTGACTGAGGTGCACG
  CAGCGTCCGGGAGCCTCCCCGTCTTCGTGGTGGTGGTTCTGGTGCTCCTGGCAGTTGTGCTGGTCACCCT
  GGCAGGCATCATCGTCTACCGCAAAGCCCGGAGCCGCATCCTGAGCAGGAACGTGGCTCCCAAGACCACA
  ATGGGGCGCTCCAACCCCCTGTTCCACCAGGCTGCCAGCCGCGTGCCGGCCAAGGGCGGGGCTCCAGCCC
  CATCCAGGGGCCCCCAAGAGCTGGTCCCCACCACCCACCCGGGCCAGCCCGCCCGACACCCGGCCTCCTC
  GGTGGCTCTGAAGAGGCCGCCCCCTGCTCCTCCGGTCACTGTGTCCAGCCCACCCTTCCCAGTTCCTGTC
  TACACCCGGCAGGCACCAAAGCAGGGTGCTGTTGGCCCAAAGGTTGCCCTGAAGCCCCCCATCCAGAGGA
  AGCAAGGAGCCGGAGCTCCCACAGCACCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC228563 representing NM_001164490
  Red=Cloning site Green=Tags(s)
  
  MRGLGLWLLGAMMLPGPAPREGELRPWGHRAQLHPPPAEEQGPAGLRLHRDLYGCQWLRGDGAASRAGPL
  LLPGPRRGVPGLSRQPQHLCRPQVGSDLHLIEPLDEGGEGGRHAVYQAEHLLQTAGTCGVSDDSLGSLLG
  PRTAAVFRPRPGDSLPSRETRYVELYVVVDNAEFQMLGSEAAVRHRVLEVVNHVDKLYQKLNFRVVLVGL
  EIWNSQDRFHVSPDPSVTLENLLTWQARQRTRRHLHDNVQLITGVDFTGTTVGFARVSAMCSHSSGAVNQ
  DHSKNPVGVACTMAHEMGHNLGMDHDENVQGCRCQERFEAGRCIMAGSIGSSFPRMFSDCSQAYLESFLE
  RPQSVCLANAPDLSHLVGGPVCGNLFVERGEQCDCGPPEDCRNRCCNSTTCQLAEGAQCAHGTCCQECKV
  KPAGELCRPKKDMCDLEEFCDGRHPECPEDAFQENGTPCSGGYCYNGACPTLAQQCQAFWGPGGQAAEES
  CFSYDILPGCKASRYRADMCGVLQCKGGQQPLGRAICIVDVCHALTTEDGTAYEPVPEGTRCGPEKVCNH
  KQECHCHAGWAPPHCAKLLTEVHAASGSLPVFVVVVLVLLAVVLVTLAGIIVYRKARSRILSRNVAPKTT
  MGRSNPLFHQAASRVPAKGGAPAPSRGPQELVPTTHPGQPARHPASSVALKRPPPAPPVTVSSPPFPVPV
  YTRQAPKQGAVGPKVALKPPIQRKQGAGAPTAP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001164490
• ORF Size: 2199 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001164490.2
• RefSeq ORF: 2202 bp
• Locus ID: 101
• UniProt ID: P78325
• Cytogenetics: 10q26.3
• Protein Families: Druggable Genome, Transmembrane
• MW: 78.7 kDa
• Gene Summary: This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. The protein encoded by this gene may be involved in cell adhesion during neurodegeneration, and it is thought to be a target for allergic respiratory diseases, including asthma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2009]