IRAKM (IRAK3) (NM_001142523) Human Tagged ORF Clone

CAT#: RC227439

IRAK3 (Myc-DDK-tagged)-Human interleukin-1 receptor-associated kinase 3 (IRAK3), transcript variant 2

Product Images

Circular map for RC227439

• 

  Western blot validation of overexpression lysate (Cat# LY428144) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC227439 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: IRAKM
• Synonyms: ASRT5; IRAKM
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC227439 representing NM_001142523
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGGGAACTGTGGGGCCCGCGGCGCGCTGTCGGCGCACACGCTGCTGTTCGACCTGCCGCCCGCGC
  TGCTCGGAGAGCTCTGCGCTGTTCTGGACAGCTGCGACGGCGCGCTGGGCTGGCGCGGCCTGGGAGCAGT
  GTTGAGTCCTTCAGAGAAGAGTTATCAGGAAGGTGGATTTCCAAATATATTATTCAAGGAAACAGCCAAT
  GTCACCGTGGATAATGTTCTTATTCCTGAACATAATGAAAAAGGAATACTGCTTAAATCTTCCATCAGCT
  TTCAAAATATCATAGAAGGAACTAGAAATTTCCACAAAGACTTCCTAATTGGAGAAGGAGAGATTTTTGA
  GGTATACAGAGTGGAGATTCAAAACCTAACATATGCTGTCAAATTATTTAAACAGGAGAAAAAAATGCAG
  TGTAAGAAGCATTGGAAGAGGTTTTTATCTGAGCTTGAAGTTTTACTACTGTTTCATCACCCAAACATAC
  TAGAGTTGGCTGCATATTTTACAGAGACTGAGAAGTTCTGTCTGATTTATCCATACATGAGAAATGGAAC
  ACTTTTTGACAGATTGCAGTGTGTAGGTGACACGGCCCCACTCCCTTGGCACATTCGAATCGGTATATTA
  ATAGGAATATCCAAAGCCATTCACTACCTGCACAACGTTCAACCATGCTCGGTCATCTGTGGCAGTATAT
  CAAGTGCAAACATCCTTTTGGATGATCAGTTTCAACCCAAACTAACTGATTTTGCCATGGCACACTTCCG
  GTCCCACCTAGAACATCAGAGTTGTACCATAAATATGACCAGCAGCAGCAGTAAACATCTGTGGTACATG
  CCAGAAGAGTACATCAGACAGGGGAAACTTTCCATTAAAACAGATGTCTACAGCTTTGGAATTGTAATAA
  TGGAAGTTCTAACAGGATGTAGAGTAGTGTTAGATGATCCAAAACATATCCAGCTGCGGGATCTCCTTAG
  AGAATTGATGGAGAAGAGAGGCCTGGATTCATGTCTCTCATTTCTAGATAAGAAAGTGCCTCCCTGCCCT
  CGGAATTTCTCTGCCAAGCTCTTCTGTTTGGCAGGCCGGTGTGCTGCAACGCGGGCAAAGTTAAGACCAT
  CAATGGATGAAGTTTTAAATACTCTTGAAAGTACTCAAGCCAGCTTGTATTTTGCTGAAGATCCTCCCAC
  ATCACTAAAGTCCTTCAGGTGTCCTTCTCCTCTATTCCTGGAGAATGTACCAAGTATTCCAGTGGAAGAT
  GATGAAAGCCAGAATAACAATTTACTACCTTCTGATGAAGGCCTGAGGATAGACAGAATGACTCAGAAAA
  CTCCTTTTGAATGCAGCCAGTCTGAGGTTATGTTTCTGAGCTTGGACAAAAAGCCAGAGAGCAAGAGAAA
  TGAGGAAGCTTGCAACATGCCCAGTTCTTCTTGTGAAGAAAGTTGGTTCCCAAAGTATATAGTTCCATCC
  CAGGACTTAAGGCCCTATAAGGTAAATATAGATCCTTCTTCAGAAGCTCCAGGGCATTCTTGCAGGAGCA
  GGCCAGTGGAGAGCAGCTGTTCCTCCAAATTTTCCTGGGATGAATATGAACAGTACAAAAAAGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC227439 representing NM_001142523
  Red=Cloning site Green=Tags(s)
  
  MAGNCGARGALSAHTLLFDLPPALLGELCAVLDSCDGALGWRGLGAVLSPSEKSYQEGGFPNILFKETAN
  VTVDNVLIPEHNEKGILLKSSISFQNIIEGTRNFHKDFLIGEGEIFEVYRVEIQNLTYAVKLFKQEKKMQ
  CKKHWKRFLSELEVLLLFHHPNILELAAYFTETEKFCLIYPYMRNGTLFDRLQCVGDTAPLPWHIRIGIL
  IGISKAIHYLHNVQPCSVICGSISSANILLDDQFQPKLTDFAMAHFRSHLEHQSCTINMTSSSSKHLWYM
  PEEYIRQGKLSIKTDVYSFGIVIMEVLTGCRVVLDDPKHIQLRDLLRELMEKRGLDSCLSFLDKKVPPCP
  RNFSAKLFCLAGRCAATRAKLRPSMDEVLNTLESTQASLYFAEDPPTSLKSFRCPSPLFLENVPSIPVED
  DESQNNNLLPSDEGLRIDRMTQKTPFECSQSEVMFLSLDKKPESKRNEEACNMPSSSCEESWFPKYIVPS
  QDLRPYKVNIDPSSEAPGHSCRSRPVESSCSSKFSWDEYEQYKKE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001142523
• ORF Size: 1605 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001142523.2
• RefSeq ORF: 1608 bp
• Locus ID: 11213
• UniProt ID: Q9Y616
• Cytogenetics: 12q14.3
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Apoptosis, Neurotrophin signaling pathway
• MW: 60.4 kDa
• Gene Summary: This gene encodes a member of the interleukin-1 receptor-associated kinase protein family. Members of this family are essential components of the Toll/IL-R immune signal transduction pathways. This protein is primarily expressed in monocytes and macrophages and functions as a negative regulator of Toll-like receptor signaling. Mutations in this gene are associated with a susceptibility to asthma. Alternate splicing results in multiple transcript variants. [provided by RefSeq, May 2010]