ACOX3 (NM_001101667) Human Tagged ORF Clone

CAT#: RC222573

ACOX3 (Myc-DDK-tagged)-Human acyl-CoA oxidase 3, pristanoyl (ACOX3), transcript variant 2

Product Images

Circular map for RC222573

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ACOX3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC222573 representing NM_001101667
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCATCCACTGTGGAAGGAGGCGACACAGCTCTGCTCCCAGAATTCCCCAGGGGGCCCCTCGATGCCT
  ACCGAGCAAGAGCGTCCTTCAGCTGGAAGGAGCTGGCGCTGTTCACGGAAGGGGAGGGCATGCTCCGCTT
  TAAGAAAACCATCTTCTCAGCTCTTGAGAATGACCCTCTTTTCGCTCGTTCCCCTGGAGCCGATCTGTCC
  TTGGAGAAGTATCGCGAGCTGAACTTCCTTCGATGCAAGCGGATCTTCGAGTATGACTTCCTCAGTGTCG
  AAGACATGTTCAAGAGCCCTCTGAAGGTCCCCGCCTTGATTCAGTGCCTGGGCATGTATGACTCTTCTCT
  GGCTGCCAAGTACCTCCTCCATAGCTTGGTTTTTGGATCAGCAGTTTACAGTTCTGGTTCTGAAAGACAT
  CTCACATATATTCAAAAGATCTTCAGGATGGAGATTTTTGGATGTTTTGCTCTGACCGAATTAAGCCACG
  GCAGTAATACCAAGGCCATTCGCACAACTGCCCACTACGATCCTGCCACTGAGGAATTCATCATACATTC
  CCCTGATTTCGAAGCTGCCAAGTTTTGGGTTGGCAACATGGGCAAGACAGCCACTCACGCGGTGGTGTTT
  GCTAAGCTGTGTGTGCCAGGGGACCAGTGCCATGGGCTGCATCCCTTTATCGTGCAGATCCGGGACCCGA
  AGACCCTTCTTCCCATGCCTGGAGTGATGGTTGGCGACATAGGAAAAAAACTCGGGCAGAACGGTCTGGA
  TAATGGTTTCGCCATGTTCCACAAGGTCAGAGTTCCTCGCCAGAGCCTTCTGAACCGGATGGGAGACGTC
  ACCCCCGAGGGCACCTATGTCAGCCCCTTTAAGGACGTCAGGCAGCGCTTTGGAGCGTCCCTGGGGAGCC
  TGTCCTCGGGCCGGGTCTCCATCGTGAGCCTGGCCATCCTTAACCTAAAGCTGGCCGTGGCCATCGCTCT
  TCGCTTCTCAGCCACTCGGCGTCAGTTTGGACCCACAGAGGAGGAGGAAATACCAGTGCTTGAGTATCCA
  ATGCAGCAATGGCGCTTGCTTCCATATCTGGCAGCTGTCTACGCCTTAGACCATTTCTCCAAGTCGCTCT
  TCCTGGACCTGGTGGAGCTCCAGCGAGGACTTGCATCGGGAGACCGCAGCGCCAGACAGGCAGAGCTTGG
  ACGTGAGATCCACGCCCTGGCATCGGCCAGCAAGCCCCTGGCCTCGTGGACCACCCAGCAAGGAATTCAG
  GAATGCCGGGAGGCGTGTGGAGGACACGGCTATCTGGCCATGAACCGGTTGGGTGTCCTTAGAGATGACA
  ACGATCCCAACTGCACATACGAAGGTGACAACAACATCCTGCTGCAGCAGACAAGCAACTATTTGCTGGG
  TCTCCTGGCACACCAGGTCCACGATGGAGCTTGCTTCCGCAGTCCGCTGAAGTCAGTGGACTTTCTGGAC
  GCCTATCCCGGCATCCTTGACCAGAAGTTTGAGGTCTCCAGTGTTGCCGACTGCTTGGACTCTGCAGTCG
  CCCTGGCAGCATACAAGTGGCTGGTTTGCTACCTGCTCCGAGAGACTTATCAAAAATTAAACCAAGAGAA
  AAGATCAGGAAGCAGTGACTTTGAAGCAAGGAACAAATGCCAGGTGTCCCACGGCCGTCCGTTGGCGCTG
  GCCTTCGTGGAGCTCACGGTGGTCCAGAGGTTCCACGAGCACGTGCACCAGCCTTCCGTGCCGCCCTCGC
  TGCGGGCCGTGCTGGGGCGGCTCAGTGCTCTGTACGCCCTGTGGTCCCTGAGCCGCCACGCGGCCCTGCT
  CTACCGAGCTGAAAGACGATGCAGTTGCCCTGGTAGACGTGATCGCTCCTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC222573 representing NM_001101667
  Red=Cloning site Green=Tags(s)
  
  MASTVEGGDTALLPEFPRGPLDAYRARASFSWKELALFTEGEGMLRFKKTIFSALENDPLFARSPGADLS
  LEKYRELNFLRCKRIFEYDFLSVEDMFKSPLKVPALIQCLGMYDSSLAAKYLLHSLVFGSAVYSSGSERH
  LTYIQKIFRMEIFGCFALTELSHGSNTKAIRTTAHYDPATEEFIIHSPDFEAAKFWVGNMGKTATHAVVF
  AKLCVPGDQCHGLHPFIVQIRDPKTLLPMPGVMVGDIGKKLGQNGLDNGFAMFHKVRVPRQSLLNRMGDV
  TPEGTYVSPFKDVRQRFGASLGSLSSGRVSIVSLAILNLKLAVAIALRFSATRRQFGPTEEEEIPVLEYP
  MQQWRLLPYLAAVYALDHFSKSLFLDLVELQRGLASGDRSARQAELGREIHALASASKPLASWTTQQGIQ
  ECREACGGHGYLAMNRLGVLRDDNDPNCTYEGDNNILLQQTSNYLLGLLAHQVHDGACFRSPLKSVDFLD
  AYPGILDQKFEVSSVADCLDSAVALAAYKWLVCYLLRETYQKLNQEKRSGSSDFEARNKCQVSHGRPLAL
  AFVELTVVQRFHEHVHQPSVPPSLRAVLGRLSALYALWSLSRHAALLYRAERRCSCPGRRDRSS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001101667
• ORF Size: 1872 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001101667.2
• RefSeq Size: 2822 bp
• RefSeq ORF: 1875 bp
• Locus ID: 8310
• UniProt ID: O15254
• Cytogenetics: 4p16.1
• Protein Pathways: alpha-Linolenic acid metabolism, Biosynthesis of unsaturated fatty acids, Fatty acid metabolism, Metabolic pathways, PPAR signaling pathway
• MW: 69.4 kDa
• Gene Summary: Acyl-Coenzyme A oxidase 3 also know as pristanoyl -CoA oxidase (ACOX3)is involved in the desaturation of 2-methyl branched fatty acids in peroxisomes. Unlike the rat homolog, the human gene is expressed in very low amounts in liver such that its mRNA was undetectable by routine Northern-blot analysis or its product by immunoblotting or by enzyme activity measurements. However the human cDNA encoding a 700 amino acid protein with a peroxisomal targeting C-terminal tripeptide S-K-L was isolated and is thought to be expressed under special conditions such as specific developmental stages or in a tissue specific manner in tissues that have not yet been examined. [provided by RefSeq, Jul 2008]