ADAM17 (NM_003183) Human Tagged ORF Clone

CAT#: RC222457

ADAM17 (Myc-DDK-tagged)-Human ADAM metallopeptidase domain 17 (ADAM17). Note: ORF is codon optimized

Product Images

Circular map for RC222457

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ADAM17
• Synonyms: ADAM18; CD156B; CSVP; NISBD; NISBD1; TACE
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC222457 ORF sequence, codon optimized.
  Due to the complexity of NM_003183, the ORF clone is codon optimized for mammalian Expression.
  The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical.
  
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGACAGTCCCTCCTCTTCCTTACCAGTGTGGTACCATTCGTGCTTGCCCCTAGACCCCCAGACGACC
  CAGGCTTCGGACCTCATCAAAGACTGGAGAAACTCGACTCACTTCTCTCCGACTATGATATCCTGAGCCT
  TAGCAATATCCAACAACACTCCGTGAGGAAGCGGGACCTGCAGACTTCAACTCATGTGGAAACACTGCTG
  ACGTTTTCCGCACTGAAGAGGCATTTCAAGCTCTACCTTACATCTAGCACTGAGCGCTTCTCTCAAAACT
  TTAAGGTGGTGGTTGTAGACGGAAAAAACGAGAGCGAATATACAGTCAAGTGGCAAGATTTTTTCACGGG
  CCACGTTGTCGGCGAACCAGATAGCAGGGTGCTGGCGCATATTCGCGACGACGACGTGATAATCAGAATC
  AACACCGACGGAGCTGAGTACAACATCGAGCCCCTTTGGCGGTTCGTGAATGACACAAAAGACAAGCGGA
  TGCTTGTATACAAGAGCGAGGACATCAAAAATGTGTCAAGGCTCCAGTCCCCCAAGGTGTGCGGGTACCT
  GAAGGTGGATAATGAGGAGCTTCTGCCTAAAGGCCTCGTTGACCGAGAACCCCCGGAGGAACTGGTACAC
  CGGGTAAAAAGGCGGGCAGACCCAGATCCCATGAAAAATACCTGTAAGCTGCTGGTCGTGGCCGATCATA
  GATTCTACAGGTACATGGGTAGAGGCGAGGAATCTACAACAACAAACTACCTGATCGAACTGATTGATCG
  CGTGGATGACATCTATAGGAATACGAGCTGGGACAACGCTGGCTTCAAGGGATACGGGATTCAGATTGAG
  CAGATCCGAATACTGAAATCACCACAGGAAGTGAAACCTGGAGAGAAGCATTACAACATGGCGAAGTCCT
  ATCCCAACGAGGAAAAAGATGCTTGGGATGTAAAGATGTTGCTCGAGCAGTTCTCTTTCGATATAGCTGA
  GGAAGCAAGCAAAGTCTGCCTCGCCCACCTGTTTACCTACCAAGATTTTGACATGGGTACCTTGGGTCTT
  GCCTACGTTGGCTCACCGCGCGCGAATAGCCACGGCGGCGTTTGCCCCAAGGCGTATTACTCACCCGTTG
  GTAAGAAGAACATTTACCTCAACTCAGGGCTTACTTCCACTAAAAACTATGGAAAAACCATCCTTACCAA
  GGAGGCTGACCTTGTTACGACCCACGAGCTTGGCCATAATTTCGGGGCTGAGCACGACCCAGACGGCTTG
  GCTGAGTGTGCCCCAAATGAGGACCAGGGCGGAAAATATGTCATGTATCCAATCGCGGTCAGTGGCGACC
  ATGAAAACAACAAAATGTTTTCAAATTGCTCCAAACAAAGTATCTACAAAACCATTGAATCTAAAGCGCA
  GGAGTGCTTTCAAGAGAGGTCAAATAAAGTTTGTGGTAATTCCCGGGTGGACGAGGGAGAAGAATGCGAT
  CCAGGGATCATGTATCTCAATAATGATACTTGTTGTAACTCCGACTGCACGCTGAAGGAAGGGGTGCAAT
  GCTCCGACCGGAATAGTCCCTGTTGCAAGAATTGCCAGTTCGAGACTGCGCAGAAAAAGTGCCAGGAGGC
  TATTAATGCAACCTGCAAAGGTGTCTCCTATTGTACGGGGAACTCATCTGAGTGTCCACCCCCTGGCAAT
  GCCGAGGATGATACTGTGTGTTTGGATCTTGGTAAGTGCAAGGATGGGAAGTGTATCCCATTCTGCGAGC
  GGGAGCAACAGTTGGAATCATGCGCCTGCAATGAGACTGATAATTCTTGCAAAGTCTGCTGTCGCGACCT
  CAGTGGCCGCTGTGTCCCGTACGTAGATGCCGAACAAAAGAACTTGTTCCTGCGAAAGGGAAAACCTTGC
  ACCGTGGGGTTCTGCGATATGAACGGAAAATGTGAAAAAAGAGTGCAAGACGTCATCGAGAGATTTTGGG
  ATTTCATTGACCAGCTGAGCATTAATACATTTGGTAAGTTTCTCGCAGATAACATCGTGGGATCCGTCCT
  CGTCTTTAGCTTGATTTTCTGGATCCCGTTCAGTATTCTTGTCCACTGTGTGGATAAGAAACTTGACAAG
  CAGTATGAGTCTTTGAGCCTGTTTCATCCATCCAACGTGGAAATGCTGAGCAGTATGGATAGCGCATCAG
  TGAGGATCATTAAGCCTTTCCCAGCACCTCAAACTCCTGGCCGCCTGCAGCCAGCGCCAGTGATCCCTTC
  CGCGCCTGCTGCTCCAAAACTGGATCACCAAAGAATGGACACTATCCAGGAAGACCCCTCTACCGATAGC
  CATATGGATGAAGACGGTTTCGAAAAGGACCCATTCCCGAACTCATCTACTGCTGCAAAATCCTTCGAAG
  ATCTCACAGATCACCCAGTTACTAGATCCGAGAAGGCTGCAAGTTTTAAGCTCCAGCGCCAGAACAGGGT
  CGATTCTAAGGAAACTGAGTGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC222457 representing NM_003183
  Red=Cloning site Green=Tags(s)
  
  MRQSLLFLTSVVPFVLAPRPPDDPGFGPHQRLEKLDSLLSDYDILSLSNIQQHSVRKRDLQTSTHVETLL
  TFSALKRHFKLYLTSSTERFSQNFKVVVVDGKNESEYTVKWQDFFTGHVVGEPDSRVLAHIRDDDVIIRI
  NTDGAEYNIEPLWRFVNDTKDKRMLVYKSEDIKNVSRLQSPKVCGYLKVDNEELLPKGLVDREPPEELVH
  RVKRRADPDPMKNTCKLLVVADHRFYRYMGRGEESTTTNYLIELIDRVDDIYRNTSWDNAGFKGYGIQIE
  QIRILKSPQEVKPGEKHYNMAKSYPNEEKDAWDVKMLLEQFSFDIAEEASKVCLAHLFTYQDFDMGTLGL
  AYVGSPRANSHGGVCPKAYYSPVGKKNIYLNSGLTSTKNYGKTILTKEADLVTTHELGHNFGAEHDPDGL
  AECAPNEDQGGKYVMYPIAVSGDHENNKMFSNCSKQSIYKTIESKAQECFQERSNKVCGNSRVDEGEECD
  PGIMYLNNDTCCNSDCTLKEGVQCSDRNSPCCKNCQFETAQKKCQEAINATCKGVSYCTGNSSECPPPGN
  AEDDTVCLDLGKCKDGKCIPFCEREQQLESCACNETDNSCKVCCRDLSGRCVPYVDAEQKNLFLRKGKPC
  TVGFCDMNGKCEKRVQDVIERFWDFIDQLSINTFGKFLADNIVGSVLVFSLIFWIPFSILVHCVDKKLDK
  QYESLSLFHPSNVEMLSSMDSASVRIIKPFPAPQTPGRLQPAPVIPSAPAAPKLDHQRMDTIQEDPSTDS
  HMDEDGFEKDPFPNSSTAAKSFEDLTDHPVTRSEKAASFKLQRQNRVDSKETEC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003183
• ORF Size: 2472 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003183.4, NP_003174.3
• RefSeq Size: 4391 bp
• RefSeq ORF: 2475 bp
• Locus ID: 6868
• UniProt ID: P78536
• Cytogenetics: 2p25.1
• Protein Families: Druggable Genome, Protease, Transmembrane
• Protein Pathways: Alzheimer's disease, Epithelial cell signaling in Helicobacter pylori infection, Notch signaling pathway
• MW: 93 kDa
• Gene Summary: This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biologic processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. The encoded preproprotein is proteolytically processed to generate the mature protease. The encoded protease functions in the ectodomain shedding of tumor necrosis factor-alpha, in which soluble tumor necrosis factor-alpha is released from the membrane-bound precursor. This protease also functions in the processing of numerous other substrates, including cell adhesion proteins, cytokine and growth factor receptors and epidermal growth factor (EGF) receptor ligands, and plays a prominent role in the activation of the Notch signaling pathway. Elevated expression of this gene has been observed in specific cell types derived from psoriasis, rheumatoid arthritis, multiple sclerosis and Crohn's disease patients, suggesting that the encoded protein may play a role in autoimmune disease. Additionally, this protease may play a role in viral infection through its cleavage of ACE2, the cellular receptor for SARS-CoV and SARS-CoV-2. [provided by RefSeq, Aug 2020]