RALGDS (NM_001042368) Human Tagged ORF Clone

CAT#: RC222398

RALGDS (Myc-DDK-tagged)-Human ral guanine nucleotide dissociation stimulator (RALGDS), transcript variant 2

Product Images

Circular map for RC222398

• 

  Western blot validation of overexpression lysate (Cat# LY420857) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC222398 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: RALGDS
• Synonyms: RalGEF; RGDS; RGF
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC222398 representing NM_001042368
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGGTAGATTGCCAGAGCTCCACGCAGGAGATCGGTGAGGAGCTGATCAACGGAGTCATCTACTCCA
  TCTCCCTGCGCAAGGTGCAGCTGCACCACGGAGGCAACAAGGGGCAGCGCTGGCTCGGGTATGAGAATGA
  GTCGGCCCTGAACCTTTATGAGACTTGCAAGGTGCGGACCGTGAAGGCTGGCACGCTGGAGAAGCTGGTG
  GAGCACCTGGTGCCAGCCTTCCAGGGCAGCGACCTCTCCTACGTCACCATCTTCCTGTGTACCTATAGAG
  CCTTCACCACCACCCAACAGGTCCTGGACCTGCTGTTCAAAAGGTACGGTAGATGTGACGCCCTCACGGC
  CTCCTCTAGATACGGCTGCATCCTCCCCTATTCCGACGAGGATGGTGGACCCCAGGACCAACTTAAAAAT
  GCCATCTCCTCCATCCTGGGCACCTGGCTGGACCAGTACTCGGAGGATTTCTGTCAACCTCCGGACTTTC
  CCTGCCTCAAGCAGCTGGTGGCCTACGTGCAGCTCAACATGCCAGGCTCAGACCTGGAGCGCCGTGCCCA
  CCTTCTCCTGGCCCAGCTGGAGCACTCGGAACCCATTGAGGCAGAGCCTGAGGCTCTGTCACCAGTGCCA
  GCTCTAAAACCAACTCCAGAGCTCGAGCTAGCTCTAACACCAGCTCGAGCACCCAGCCCAGTGCCGGCTC
  CAGCCCCGGAGCCAGAGCCAGCTCCAACACCAGCTCCAGGTTCAGAGCTAGAAGTAGCTCCAGCACCAGC
  TCCGGAGCTCCAGCAGGCTCCAGAGCCAGCTGTGGGACTAGAATCGGCTCCAGCGCCAGCTCTGGAACTA
  GAGCCAGCTCCAGAACAGGATCCAGCTCCCTCACAAACTCTAGAGCTGGAGCCAGCTCCAGCACCAGTTC
  CATCATTACAGCCTTCCTGGCCTTCACCTGTGGTTGCAGAGAACGGGCTGAGTGAGGAGAAGCCTCACCT
  CTTGGTGTTCCCTCCAGATCTGGTGGCAGAGCAGTTTACACTGATGGATGCGGAACTGTTCAAGAAGGTG
  GTGCCCTACCACTGCCTGGGCTCCATCTGGTCCCAGCGGGACAAGAAGGGCAAGGAGCACCTGGCGCCCA
  CCATCCGCGCCACTGTCACCCAGTTCAACAGTGTGGCCAACTGTGTCATCACCACCTGCCTCGGGAACCG
  AAGCACGAAAGCCCCAGACAGGGCCAGGGTGGTGGAGCACTGGATCGAGGTGGCCAGGGAGTGCCGGATC
  CTCAAGAACTTCTCGTCACTGTATGCCATCCTCTCTGCCCTGCAGAGCAACTCCATCCACCGTCTGAAGA
  AGACGTGGGAAGACGTTTCCAGGGACAGTTTCCGGATCTTTCAGAAGCTGTCAGAGATCTTCTCAGATGA
  GAACAACTACTCATTGAGCCGGGAGCTGCTCATCAAGGAGGGCACCTCCAAGTTTGCCACCCTGGAGATG
  AACCCCAAGAGAGCCCAGAAACGGCCGAAGGAGACGGGCATCATCCAGGGCACCGTTCCCTACCTGGGCA
  CGTTCCTCACCGACCTGGTGATGCTGGACACTGCCATGAAGGACTATCTGTATGGCAGACTCATCAACTT
  TGAGAAGAGGAGGAAGGAGTTCGAGGTGATCGCCCAGATCAAGCTGCTGCAGTCGGCCTGCAACAACTAC
  AGCATCGCGCCAGATGAGCAATTTGGGGCCTGGTTCCGGGCCGTGGAGCGGCTCAGCGAGACTGAGAGCT
  ACAACCTGTCGTGCGAGCTGGAGCCCCCATCCGAGTCAGCCAGCAACACCCTCAGGACCAAGAAGAACAC
  AGCCATTGTCAAGCGCTGGAGCGACCGCCAGGCCCCCAGCACTGAGCTCAGTACCAGTGGCAGCTCCCAC
  TCCAAGTCCTGTGACCAGCTCAGGTGTGGCCCCTACCTCAGCAGCGGGGACATCGCTGACGCGCTCAGCG
  TGCACTCGGCCGGCTCCTCTAGCTCCGACGTGGAGGAGATCAACATCAGCTTCGTCCCGGAGTCTCCTGA
  TGGCCAGGAAAAGAAGTTCTGGGAATCAGCCTCACAGTCATCCCCGGAGACCTCCGGCATCAGCTCAGCC
  TCCAGCAGCACCTCGTCCTCCTCAGCCTCCACCACGCCCGTGGCTGCCACACGCACCCACAAGCGCTCTG
  TCTCAGGGCTCTGCAACTCCAGCTCCGCGCTGCCGCTCTACAACCAGCAGGTGGGCGACTGCTGTATCAT
  CCGCGTCAGCCTGGACGTGGACAATGGCAACATGTACAAGAGCATCCTGGTGACCAGCCAAGATAAGGCT
  CCGGCTGTAATCCGCAAGGCCATGGACAAACACAACCTGGAGGAGGAGGAGCCGGAGGACTATGAGCTGC
  TGCAGATTCTCTCAGATGACCGGAAGCTGAAGATCCCTGAAAACGCCAACGTCTTCTATGCCATGAACTC
  TACCGCCAACTATGACTTTGTCCTCAAGAAGCGGACCTTCACCAAGGGAGTGAAGGTCAAGCACGGAGCC
  AGCTCCACCCTCCCTCGCATGAAGCAGAAAGGACTCAAGATTGCCAAGGGCATCTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC222398 representing NM_001042368
  Red=Cloning site Green=Tags(s)
  
  MMVDCQSSTQEIGEELINGVIYSISLRKVQLHHGGNKGQRWLGYENESALNLYETCKVRTVKAGTLEKLV
  EHLVPAFQGSDLSYVTIFLCTYRAFTTTQQVLDLLFKRYGRCDALTASSRYGCILPYSDEDGGPQDQLKN
  AISSILGTWLDQYSEDFCQPPDFPCLKQLVAYVQLNMPGSDLERRAHLLLAQLEHSEPIEAEPEALSPVP
  ALKPTPELELALTPARAPSPVPAPAPEPEPAPTPAPGSELEVAPAPAPELQQAPEPAVGLESAPAPALEL
  EPAPEQDPAPSQTLELEPAPAPVPSLQPSWPSPVVAENGLSEEKPHLLVFPPDLVAEQFTLMDAELFKKV
  VPYHCLGSIWSQRDKKGKEHLAPTIRATVTQFNSVANCVITTCLGNRSTKAPDRARVVEHWIEVARECRI
  LKNFSSLYAILSALQSNSIHRLKKTWEDVSRDSFRIFQKLSEIFSDENNYSLSRELLIKEGTSKFATLEM
  NPKRAQKRPKETGIIQGTVPYLGTFLTDLVMLDTAMKDYLYGRLINFEKRRKEFEVIAQIKLLQSACNNY
  SIAPDEQFGAWFRAVERLSETESYNLSCELEPPSESASNTLRTKKNTAIVKRWSDRQAPSTELSTSGSSH
  SKSCDQLRCGPYLSSGDIADALSVHSAGSSSSDVEEINISFVPESPDGQEKKFWESASQSSPETSGISSA
  SSSTSSSSASTTPVAATRTHKRSVSGLCNSSSALPLYNQQVGDCCIIRVSLDVDNGNMYKSILVTSQDKA
  PAVIRKAMDKHNLEEEEPEDYELLQILSDDRKLKIPENANVFYAMNSTANYDFVLKKRTFTKGVKVKHGA
  SSTLPRMKQKGLKIAKGIF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001042368
• ORF Size: 2577 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001042368.3
• RefSeq Size: 3668 bp
• RefSeq ORF: 2580 bp
• Locus ID: 5900
• UniProt ID: Q12967
• Cytogenetics: 9q34.13-q34.2
• Protein Pathways: Colorectal cancer, Pancreatic cancer, Pathways in cancer
• MW: 94.5 kDa
• Gene Summary: Guanine nucleotide dissociation stimulators (GDSs, or exchange factors), such as RALGDS, are effectors of Ras-related GTPases (see MIM 190020) that participate in signaling for a variety of cellular processes.[supplied by OMIM, Nov 2010]