FOXL1 (NM_005250) Human Tagged ORF Clone

CAT#: RC216442

FOXL1 (Myc-DDK-tagged)-Human forkhead box L1 (FOXL1)

Product Images

Circular map for RC216442

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  Western blot validation of overexpression lysate (Cat# LY417423) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC216442 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified FOXL1 protein (Cat# TP316442). The protein was produced from HEK293T cells transfected with FOXL1 cDNA clone (Cat# RC216442) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: FOXL1
• Synonyms: FKH6; FKHL11; FREAC7
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC216442 representing NM_005250
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTCACCTCTTCGATCCCCGGCTGCCTGCCCTGGCCGCCTCGCCCATGCTGTATCTGTACGGTCCCG
  AGAGACCCGGCCTCCCTCTGGCCTTCGCCCCCGCGGCTGCTCTAGCTGCCTCGGGCCGGGCCGAGACCCC
  GCAGAAGCCTCCCTACAGCTACATCGCGCTCATCGCCATGGCGATCCAGGACGCGCCCGAGCAGAGGGTC
  ACGCTCAACGGCATCTACCAGTTCATCATGGACCGCTTCCCCTTCTACCACGACAACCGGCAGGGCTGGC
  AGAACAGCATCCGCCACAACCTCTCGCTCAACGACTGCTTCGTCAAGGTGCCCCGCGAGAAAGGGCGGCC
  GGGCAAGGGCAGCTACTGGACGCTGGACCCCCGCTGCCTGGACATGTTTGAGAACGGCAACTACCGGCGC
  CGGAAGAGGAAGCCCAAGCCGGGCCCCGGGGCCCCGGAGGCCAAGAGGCCCCGCGCCGAGACGCACCAGC
  GCAGCGCGGAGGCGCAGCCGGAGGCGGGGAGCGGGGCAGGGGGCTCGGGCCCCGCAATCTCCCGCCTGCA
  GGCAGCGCCCGCGGGCCCCTCGCCCCTCCTGGACGGCCCCTCTCCGCCGGCGCCCCTCCACTGGCCGGGG
  ACCGCGTCCCCGAACGAGGACGCTGGTGACGCTGCCCAGGGCGCAGCGGCCGTGGCGGTCGGCCAGGCAG
  CGCGCACAGGGGACGGCCCGGGGTCCCCTCTGCGCCCCGCCTCCCGCAGCTCTCCGAAGAGCTCCGACAA
  GTCCAAGAGCTTCAGCATAGACAGCATCCTGGCGGGAAAGCAGGGCCAGAAGCCGCCTTCAGGGGACGAA
  CTCCTAGGGGGTGCCAAGCCTGGGCCCGGCGGCCGTCTGGGTGCCTCGCTCCTGGCCGCCTCCTCCAGCC
  TCCGTCCGCCTTTCAACGCTTCCCTGATGCTCGACCCGCATGTCCAGGGCGGCTTTTACCAGCTCGGGAT
  CCCCTTCCTCTCTTATTTCCCCCTGCAGGTTCCCGACACGGTACTCCACTTCCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC216442 representing NM_005250
  Red=Cloning site Green=Tags(s)
  
  MSHLFDPRLPALAASPMLYLYGPERPGLPLAFAPAAALAASGRAETPQKPPYSYIALIAMAIQDAPEQRV
  TLNGIYQFIMDRFPFYHDNRQGWQNSIRHNLSLNDCFVKVPREKGRPGKGSYWTLDPRCLDMFENGNYRR
  RKRKPKPGPGAPEAKRPRAETHQRSAEAQPEAGSGAGGSGPAISRLQAAPAGPSPLLDGPSPPAPLHWPG
  TASPNEDAGDAAQGAAAVAVGQAARTGDGPGSPLRPASRSSPKSSDKSKSFSIDSILAGKQGQKPPSGDE
  LLGGAKPGPGGRLGASLLAASSSLRPPFNASLMLDPHVQGGFYQLGIPFLSYFPLQVPDTVLHFQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005250
• ORF Size: 1035 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005250.3
• RefSeq Size: 1038 bp
• RefSeq ORF: 1038 bp
• Locus ID: 2300
• UniProt ID: Q12952
• Cytogenetics: 16q24.1
• Protein Families: Transcription Factors
• MW: 36.3 kDa
• Gene Summary: This gene encodes a member of the forkhead/winged helix-box (FOX) family of transcription factors. FOX transcription factors are characterized by a distinct DNA-binding forkhead domain and play critical roles in the regulation of multiple processes including metabolism, cell proliferation and gene expression during ontogenesis. [provided by RefSeq, Nov 2012]