ABCB5 (NM_178559) Human Tagged ORF Clone

CAT#: RC215604

ABCB5 (Myc-DDK-tagged)-Human ATP-binding cassette, sub-family B (MDR/TAP), member 5 (ABCB5), transcript variant 2

Product Images

Circular map for RC215604

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ABCB5
• Synonyms: ABCB5alpha; ABCB5beta; EST422562
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC215604 representing NM_178559
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGGATGAGAATGACATCAGAGCTTTAAATGTGCGGCATTATCGAGACCATATTGGAGTGGTTAGTC
  AAGAGCCTGTTTTGTTCGGGACCACCATCAGTAACAATATCAAGTATGGACGAGATGATGTGACTGATGA
  AGAGATGGAGAGAGCAGCAAGGGAAGCAAATGCGTATGATTTTATCATGGAGTTTCCTAATAAATTTAAT
  ACATTGGTAGGGGAAAAAGGAGCTCAAATGAGTGGAGGGCAGAAACAGAGGATCGCAATTGCTCGTGCCT
  TAGTTCGAAACCCCAAGATTCTGATTTTAGATGAGGCTACGTCTGCCCTGGATTCAGAAAGCAAGTCAGC
  TGTTCAAGCTGCACTGGAGAAGGCGAGCAAAGGTCGGACTACAATCGTGGTAGCACACCGACTTTCTACT
  ATTCGAAGTGCAGATTTGATTGTGACCCTAAAGGATGGAATGCTGGCGGAGAAAGGAGCACATGCTGAAC
  TAATGGCAAAACGAGGTCTATATTATTCACTTGTGATGTCACAGGATATTAAAAAAGCTGATGAACAGAT
  GGAGTCAATGACATATTCTACTGAAAGAAAGACCAACTCACTTCCTCTGCACTCTGTGAAGAGCATCAAG
  TCAGACTTCATTGACAAGGCTGAGGAATCCACCCAATCTAAAGAGATAAGTCTTCCTGAAGTCTCTCTAT
  TAAAAATTTTAAAGTTAAACAAGCCTGAATGGCCTTTTGTGGTTCTGGGGACATTGGCTTCTGTTCTAAA
  TGGAACTGTTCATCCAGTATTTTCCATCATCTTTGCAAAAATTATAACCATGTTTGGAAATAATGATAAA
  ACCACATTAAAGCATGATGCAGAAATTTATTCCATGATATTCGTCATTTTGGGTGTTATTTGCTTTGTCA
  GTTATTTCATGCAGGGATTATTTTACGGCAGAGCAGGGGAAATTTTAACGATGAGATTAAGACACTTGGC
  CTTCAAAGCCATGTTATATCAGGATATTGCCTGGTTTGATGAAAAGGAAAACAGCACAGGAGGCTTGACA
  ACAATATTAGCCATAGATATAGCACAAATTCAAGGAGCAACAGGTTCCAGGATTGGCGTCTTAACACAAA
  ATGCAACTAACATGGGACTTTCAGTTATCATTTCCTTTATATATGGATGGGAGATGACATTCCTGATTCT
  GAGTATTGCTCCAGTACTTGCCGTGACAGGAATGATTGAAACCGCAGCAATGACTGGATTTGCCAACAAA
  GATAAGCAAGAACTTAAGCATGCTGGAAAGATAGCAACTGAAGCTTTGGAGAATATACGTACTATAGTGT
  CATTAACAAGGGAAAAAGCCTTCGAGCAAATGTATGAAGAGATGCTTCAGACTCAACACAGAAATACCTC
  GAAGAAAGCACAGATTATTGGAAGCTGTTATGCATTCAGCCATGCCTTTATATATTTTGCCTATGCGGCA
  GGGTTTCGATTTGGAGCCTATTTAATTCAAGCTGGACGAATGACCCCAGAGGGCATGTTCATAGTTTTTA
  CTGCAATTGCATATGGAGCTATGGCCATCGGAGAAACGCTCGTTTTGGCTCCTGAATATTCCAAAGCCAA
  ATCGGGGGCTGCGCATCTGTTTGCCTTGTTGGAAAAGAAACCAAATATAGACAGCCGCAGTCAAGAAGGG
  AAAAAGCCAGACACATGTGAAGGGAATTTAGAGTTTCGAGAAGTCTCTTTCTTCTATCCATGTCGCCCAG
  ATGTTTTCATCCTCCGTGGCTTATCCCTCAGTATTGAGCGAGGAAAGACAGTAGCATTTGTGGGGAGCAG
  CGGCTGTGGGAAAAGCACTTCTGTTCAACTTCTGCAGAGACTTTATGACCCCGTGCAAGGACAAGTGCTG
  TTTGATGGTGTGGATGCAAAAGAATTGAATGTACAGTGGCTCCGTTCCCAAATAGCAATCGTTCCTCAAG
  AGCCTGTGCTCTTCAACTGCAGCATTGCTGAGAACATCGCCTATGGTGACAACAGCCGTGTGGTGCCATT
  AGATGAGATCAAAGAAGCCGCAAATGCAGCAAATATCCATTCTTTTATTGAAGGTCTCCCTGAGAAATAC
  AACACACAAGTTGGACTGAAAGGAGCACAGCTTTCTGGCGGCCAGAAACAAAGACTAGCTATTGCAAGGG
  CTCTTCTCCAAAAACCCAAAATTTTATTGTTGGATGAGGCCACTTCAGCCCTCGATAATGACAGTGAGAA
  GGTGGTTCAGCATGCCCTTGATAAAGCCAGGACGGGAAGGACATGCCTAGTGGTCACTCACAGGCTCTCT
  GCAATTCAGAACGCAGATTTGATAGTGGTTCTGCACAATGGAAAGATAAAGGAACAAGGAACTCATCAAG
  AGCTCCTGAGAAATCGAGACATATATTTTAAGTTAGTGAATGCACAGTCAGTGCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC215604 representing NM_178559
  Red=Cloning site Green=Tags(s)
  
  MVDENDIRALNVRHYRDHIGVVSQEPVLFGTTISNNIKYGRDDVTDEEMERAAREANAYDFIMEFPNKFN
  TLVGEKGAQMSGGQKQRIAIARALVRNPKILILDEATSALDSESKSAVQAALEKASKGRTTIVVAHRLST
  IRSADLIVTLKDGMLAEKGAHAELMAKRGLYYSLVMSQDIKKADEQMESMTYSTERKTNSLPLHSVKSIK
  SDFIDKAEESTQSKEISLPEVSLLKILKLNKPEWPFVVLGTLASVLNGTVHPVFSIIFAKIITMFGNNDK
  TTLKHDAEIYSMIFVILGVICFVSYFMQGLFYGRAGEILTMRLRHLAFKAMLYQDIAWFDEKENSTGGLT
  TILAIDIAQIQGATGSRIGVLTQNATNMGLSVIISFIYGWEMTFLILSIAPVLAVTGMIETAAMTGFANK
  DKQELKHAGKIATEALENIRTIVSLTREKAFEQMYEEMLQTQHRNTSKKAQIIGSCYAFSHAFIYFAYAA
  GFRFGAYLIQAGRMTPEGMFIVFTAIAYGAMAIGETLVLAPEYSKAKSGAAHLFALLEKKPNIDSRSQEG
  KKPDTCEGNLEFREVSFFYPCRPDVFILRGLSLSIERGKTVAFVGSSGCGKSTSVQLLQRLYDPVQGQVL
  FDGVDAKELNVQWLRSQIAIVPQEPVLFNCSIAENIAYGDNSRVVPLDEIKEAANAANIHSFIEGLPEKY
  NTQVGLKGAQLSGGQKQRLAIARALLQKPKILLLDEATSALDNDSEKVVQHALDKARTGRTCLVVTHRLS
  AIQNADLIVVLHNGKIKEQGTHQELLRNRDIYFKLVNAQSVQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_178559
• ORF Size: 2436 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_178559.6
• RefSeq Size: 2784 bp
• RefSeq ORF: 2439 bp
• Locus ID: 340273
• UniProt ID: Q2M3G0
• Cytogenetics: 7p21.1
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: ABC transporters
• MW: 89.7 kDa
• Gene Summary: ABCB5 belongs to the ATP-binding cassette (ABC) transporter superfamily of integral membrane proteins. These proteins participate in ATP-dependent transmembrane transport of structurally diverse molecules ranging from small ions, sugars, and peptides to more complex organic molecules (Chen et al., 2005 [PubMed 15760339]).[supplied by OMIM, Mar 2008]