RASGRP2 (NM_001098671) Human Tagged ORF Clone

CAT#: RC212719

RASGRP2 (Myc-DDK-tagged)-Human RAS guanyl releasing protein 2 (calcium and DAG-regulated) (RASGRP2), transcript variant 4

Product Images

Circular map for RC212719

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  Western blot validation of overexpression lysate (Cat# LY406966) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC217525 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified RASGRP2 protein (Cat# TP312719). The protein was produced from HEK293T cells transfected with RASGRP2 cDNA clone (Cat# RC212719) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: RASGRP2
• Synonyms: CALDAG-GEFI; CDC25L
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC212719 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGGCACCCTGGACCTGGACAAGGGCTGCACGGTGGAGGAGCTGCTCCGCGGGTGCATCGAAGCCT
  TCGATGACTCCGGGAAGGTGCGGGACCCGCAGCTGGTGCGCATGTTCCTCATGATGCACCCCTGGTACAT
  CCCCTCCTCTCAGCTGGCGGCCAAGCTGCTCCACATCTACCAACAATCCCGGAAGGACAACTCCAATTCC
  CTGCAGGTGAAAACGTGCCACCTGGTCAGGTACTGGATCTCCGCCTTCCCAGCGGAGTTTGACTTGAACC
  CGGAGTTGGCTGAGCAGATCAAGGAGCTGAAGGCTCTGCTAGACCAAGAAGGGAACCGACGGCACAGCAG
  CCTAATCGACATAGACAGCGTCCCTACCTACAAGTGGAAGCGGCAGGTGACTCAGCGGAACCCTGTGGGA
  CAGAAAAAGCGCAAGATGTCCCTGTTGTTTGACCACCTGGAGCCCATGGAGCTGGCGGAGCATCTCACCT
  ACTTGGAGTATCGCTCCTTCTGCAAGATCCTGTTTCAGGACTATCACAGTTTCGTGACTCATGGCTGCAC
  TGTGGACAACCCCGTCCTGGAGCGGTTCATCTCCCTCTTCAACAGCGTCTCACAGTGGGTGCAGCTCATG
  ATCCTCAGCAAACCCACAGCCCCGCAGCGGGCCCTGGTCATCACACACTTTGTCCACGTGGCGGAGAAGC
  TGCTACAGCTGCAGAACTTCAACACGCTGATGGCAGTGGTCGGGGGCCTGAGCCACAGCTCCATCTCCCG
  CCTCAAGGAGACCCACAGCCACGTTAGCCCTGAGACCATCAAGCTCTGGGAGGGTCTCACGGAACTAGTG
  ACGGCGACAGGCAACTATGGCAACTACCGGCGTCGGCTGGCAGCCTGTGTGGGCTTCCGCTTCCCGATCC
  TGGGTGTGCACCTCAAGGACCTGGTGGCCCTGCAGCTGGCACTGCCTGACTGGCTGGACCCAGCCCGGAC
  CCGGCTCAACGGGGCCAAGATGAAGCAGCTCTTTAGCATCCTGGAGGAGCTGGCCATGGTGACCAGCCTG
  CGGCCACCAGTACAGGCCAACCCCGACCTGCTGAGCCTGCTCACGGTGTCTCTGGATCAGTATCAGACGG
  AGGATGAGCTGTACCAGCTGTCCCTGCAGCGGGAGCCGCGCTCCAAGTCCTCGCCAACCAGCCCCACGAG
  TTGCACCCCACCACCCCGGCCCCCGGTACTGGAGGAGTGGACCTCGGCTGCCAAACCCAAGCTGGATCAG
  GCCCTCGTGGTGGAGCACATCGAGAAGATGGTGGAGTCTGTGTTCCGGAACTTTGACGTCGATGGGGATG
  GCCACATCTCACAGGAAGAATTCCAGATCATCCGTGGGAACTTCCCTTACCTCAGCGCCTTTGGGGACCT
  CGACCAGAACCAGGATGGCTGCATCAGCAGGGAGGAGATGGTTTCCTATTTCCTGCGCTCCAGCTCTGTG
  TTGGGGGGGCGCATGGGCTTCGTACACAACTTCCAGGAGAGCAACTCCTTGCGCCCCGTCGCCTGCCGCC
  ACTGCAAAGCCCTGATCCTGGGCATCTACAAGCAGGGCCTCAAATGCCGAGCCTGTGGAGTGAACTGCCA
  CAAGCAGTGCAAGGATCGCCTGTCAGTTGAGTGTCGGCGCAGGGCCCAGAGTGTGAGCCTGGAGGGGTCT
  GCACCCTCACCCTCACCCATGCACAGCCACCATCACCGCGCCTTCAGCTTCTCTCTGCCCCGCCCTGGCA
  GGCGAGGCTCCAGGCCTCCAGAGATCCGTGAGGAGGAGGTACAGACGGTGGAGGATGGGGTGTTTGACAT
  CCACTTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC212719 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAGTLDLDKGCTVEELLRGCIEAFDDSGKVRDPQLVRMFLMMHPWYIPSSQLAAKLLHIYQQSRKDNSNS
  LQVKTCHLVRYWISAFPAEFDLNPELAEQIKELKALLDQEGNRRHSSLIDIDSVPTYKWKRQVTQRNPVG
  QKKRKMSLLFDHLEPMELAEHLTYLEYRSFCKILFQDYHSFVTHGCTVDNPVLERFISLFNSVSQWVQLM
  ILSKPTAPQRALVITHFVHVAEKLLQLQNFNTLMAVVGGLSHSSISRLKETHSHVSPETIKLWEGLTELV
  TATGNYGNYRRRLAACVGFRFPILGVHLKDLVALQLALPDWLDPARTRLNGAKMKQLFSILEELAMVTSL
  RPPVQANPDLLSLLTVSLDQYQTEDELYQLSLQREPRSKSSPTSPTSCTPPPRPPVLEEWTSAAKPKLDQ
  ALVVEHIEKMVESVFRNFDVDGDGHISQEEFQIIRGNFPYLSAFGDLDQNQDGCISREEMVSYFLRSSSV
  LGGRMGFVHNFQESNSLRPVACRHCKALILGIYKQGLKCRACGVNCHKQCKDRLSVECRRRAQSVSLEGS
  APSPSPMHSHHHRAFSFSLPRPGRRGSRPPEIREEEVQTVEDGVFDIHL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001098671
• ORF Size: 1827 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001098671.2
• RefSeq Size: 2284 bp
• RefSeq ORF: 1830 bp
• Locus ID: 10235
• UniProt ID: Q7LDG7
• Cytogenetics: 11q13.1
• Protein Pathways: Chemokine signaling pathway, MAPK signaling pathway
• MW: 69.2 kDa
• Gene Summary: The protein encoded by this gene is a brain-enriched nucleotide exchanged factor that contains an N-terminal GEF domain, 2 tandem repeats of EF-hand calcium-binding motifs, and a C-terminal diacylglycerol/phorbol ester-binding domain. This protein can activate small GTPases, including RAS and RAP1/RAS3. The nucleotide exchange activity of this protein can be stimulated by calcium and diacylglycerol. Four alternatively spliced transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jan 2016]