Integrin alpha 1 (ITGA1) (NM_181501) Human Tagged ORF Clone

CAT#: RC211971

ITGA1 (Myc-DDK-tagged)-Human integrin, alpha 1 (ITGA1)

Product Images

Circular map for RC211971

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Integrin alpha 1
• Synonyms: CD49a; VLA1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC211971 representing NM_181501
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCCTCGGCCCCGCGCCCGCCCAGGGGTCGCTGTCGCCTGCTGCTGGCTCCTCACTGTTGTTCTAC
  GCTGCTGCGTATCATTCAATGTTGATGTGAAAAATTCAATGACTTTCAGCGGCCCGGTGGAAGACATGTT
  TGGATATACTGTTCAACAATATGAAAATGAAGAAGGAAAATGGGTGCTTATTGGTTCTCCGTTAGTTGGC
  CAACCCAAAAACAGAACTGGAGATGTCTATAAGTGTCCAGTTGGGAGAGGTGAATCATTACCTTGTGTAA
  AGTTGGATCTACCAGTTAATACATCAATTCCCAATGTCACAGAAGTAAAGGAGAACATGACATTTGGATC
  AACTTTAGTCACCAACCCAAATGGAGGATTTCTGGCTTGTGGGCCCTTATATGCCTATAGATGTGGACAT
  TTGCATTACACAACTGGAATCTGTTCTGACGTCAGCCCCACATTTCAAGTCGTGAATTCCATTGCCCCTG
  TACAAGAATGCAGCACTCAACTGGACATAGTCATAGTGCTGGATGGTTCCAACAGTATTTACCCATGGGA
  CAGTGTTACAGCTTTTTTAAATGACCTTCTTGAAAGAATGGATATTGGTCCTAAACAGACACAGGTTGGA
  ATTGTACAGTATGGAGAAAACGTGACCCATGAGTTCAACCTCAATAAGTATTCTTCCACCGAAGAGGTAC
  TTGTTGCAGCAAAGAAAATAGTCCAGAGAGGTGGCCGCCAGACTATGACAGCTCTTGGAATAGACACAGC
  AAGAAAGGAGGCATTCACGGAAGCCCGGGGTGCCCGAAGAGGAGTTAAAAAAGTCATGGTTATTGTGACA
  GATGGAGAGTCTCATGACAATCATCGACTGAAGAAGGTCATCCAAGACTGTGAAGATGAAAACATTCAAC
  GGTTTTCCATAGCTATTCTTGGCAGCTATAACCGAGGAAATTTAAGCACTGAAAAATTTGTGGAGGAAAT
  AAAATCAATTGCAAGTGAACCCACTGAAAAGCATTTCTTCAATGTCTCTGATGAATTGGCTCTAGTCACC
  ATTGTTAAAACTCTGGGAGAAAGAATATTTGCCCTGGAAGCCACAGCTGACCAGTCAGCAGCTTCATTTG
  AAATGGAAATGTCTCAGACTGGCTTCAGTGCTCATTATTCACAGGACTGGGTCATGCTTGGAGCAGTAGG
  AGCCTATGATTGGAATGGAACAGTTGTCATGCAGAAGGCTAGTCAAATCATAATCCCTCGAAACACAACC
  TTTAATGTTGAGTCTACCAAAAAGAATGAACCGCTTGCTTCTTATTTAGGTTACACTGTAAACTCTGCTA
  CTGCTTCTTCTGGAGATGTGCTCTATATTGCTGGACAGCCTCGGTACAATCATACAGGCCAGGTCATTAT
  CTACAGGATGGAAGATGGAAACATCAAAATTCTCCAGACGCTCAGTGGAGAACAGATTGGTTCCTACTTT
  GGCAGTATTTTAACAACAACTGACATTGACAAGGATTCTAATACTGACATTCTTCTAGTCGGAGCCCCTA
  TGTACATGGGAACAGAGAAGGAGGAGCAAGGAAAAGTGTATGTGTATGCTCTCAATCAGACAAGGTTTGA
  ATATCAAATGAGCCTGGAACCTATTAAGCAGACGTGCTGTTCATCTCGGCAGCACAATTCATGCACAACA
  GAAAACAAAAATGAGCCATGCGGGGCTCGTTTTGGAACTGCAATTGCTGCTGTAAAAGACCTCAATCTTG
  ATGGATTTAATGACATCGTGATAGGAGCTCCGCTGGAAGATGATCACGGGGGAGCTGTGTACATTTATCA
  TGGAAGTGGCAAGACTATAAGGAAAGAGTATGCACAACGTATTCCATCAGGTGGGGATGGTAAGACACTG
  AAATTTTTTGGCCAGTCTATCCACGGAGAAATGGATTTAAATGGTGACGGTCTGACAGATGTGACTATTG
  GGGGCCTTGGTGGTGCTGCCCTCTTCTGGTCCCGAGATGTGGCCGTAGTTAAAGTGACCATGAATTTTGA
  GCCAAATAAAGTGAATATTCAAAAGAAAAACTGCCATATGGAGGGAAAGGAAACAGTATGCATAAATGCT
  ACAGTGTGTTTTGATGTGAAATTAAAGTCTAAAGAAGACACGATTTATGAAGCTGATTTGCAGTACCGTG
  TCACCCTAGATTCACTAAGACAAATATCACGAAGTTTTTTCTCTGGAACTCAAGAGAGAAAGGTTCAAAG
  GAACATCACAGTTCGAAAATCAGAATGCACTAAGCACTCCTTCTACATGTTGGACAAGCATGACTTTCAG
  GACTCTGTGAGAATAACGTTGGACTTTAATCTTACCGATCCAGAAAATGGGCCTGTTCTTGATGATTCTC
  TACCAAACTCAGTACATGAATATATTCCCTTTGCCAAAGATTGTGGAAATAAGGAAAAATGTATCTCAGA
  CCTCAGCCTGCATGTCGCCACCACTGAAAAGGACCTGCTGATTGTCCGATCCCAGAATGATAAGTTCAAC
  GTTAGCCTCACAGTCAAAAATACAAAGGACAGTGCCTATAACACCAGGACAATAGTGCATTATTCTCCAA
  ATCTAGTTTTTTCAGGAATTGAGGCTATCCAAAAAGACAGTTGTGAATCTAATCATAATATCACATGTAA
  AGTTGGATATCCCTTCCTGAGAAGAGGAGAGATGGTAACTTTCAAAATATTGTTTCAGTTTAACACATCC
  TATCTCATGGAAAATGTGACCATTTATTTAAGTGCAACAAGTGACAGCGAAGAACCTCCTGAAACCCTTT
  CTGATAATGTAGTAAACATTTCTATCCCGGTAAAATATGAAGTTGGACTACAGTTTTACAGCTCTGCAAG
  TGAATACCACATTTCAATTGCTGCCAATGAGACAGTCCCTGAAGTTATTAATTCTACTGAGGACATTGGA
  AATGAAATTAATATCTTCTACTTGATTAGAAAAAGTGGATCTTTTCCAATGCCAGAGCTTAAGCTGTCAA
  TTTCATTCCCCAATATGACATCAAATGGTTACCCTGTGCTGTACCCAACTGGATTGTCATCTTCTGAGAA
  TGCAAACTGCAGACCCCATATCTTTGAGGATCCTTTCAGTATCAACTCTGGAAAGAAAATGACTACATCA
  ACTGACCATCTCAAACGAGGCACAATTCTGGACTGCAATACATGTAAATTTGCTACCATCACATGTAATC
  TCACTTCTTCTGACATCAGCCAAGTCAATGTTTCGCTTATCTTGTGGAAACCAACTTTTATAAAATCATA
  TTTTTCCAGCTTAAATCTTACTATAAGGGGAGAACTTCGGAGTGAAAATGCATCTCTGGTTTTAAGTAGC
  AGCAATCAAAAAAGAGAGCTTGCTATTCAAATATCCAAAGATGGGCTACCGGGCAGAGTGCCATTATGGG
  TCATCCTGCTGAGTGCTTTTGCCGGATTGTTGCTGTTAATGCTGCTCATTTTAGCACTGTGGAAGATTGG
  ATTCTTCAAAAGACCACTGAAAAAGAAAATGGAGAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC211971 representing NM_181501
  Red=Cloning site Green=Tags(s)
  
  MAPRPRARPGVAVACCWLLTVVLRCCVSFNVDVKNSMTFSGPVEDMFGYTVQQYENEEGKWVLIGSPLVG
  QPKNRTGDVYKCPVGRGESLPCVKLDLPVNTSIPNVTEVKENMTFGSTLVTNPNGGFLACGPLYAYRCGH
  LHYTTGICSDVSPTFQVVNSIAPVQECSTQLDIVIVLDGSNSIYPWDSVTAFLNDLLERMDIGPKQTQVG
  IVQYGENVTHEFNLNKYSSTEEVLVAAKKIVQRGGRQTMTALGIDTARKEAFTEARGARRGVKKVMVIVT
  DGESHDNHRLKKVIQDCEDENIQRFSIAILGSYNRGNLSTEKFVEEIKSIASEPTEKHFFNVSDELALVT
  IVKTLGERIFALEATADQSAASFEMEMSQTGFSAHYSQDWVMLGAVGAYDWNGTVVMQKASQIIIPRNTT
  FNVESTKKNEPLASYLGYTVNSATASSGDVLYIAGQPRYNHTGQVIIYRMEDGNIKILQTLSGEQIGSYF
  GSILTTTDIDKDSNTDILLVGAPMYMGTEKEEQGKVYVYALNQTRFEYQMSLEPIKQTCCSSRQHNSCTT
  ENKNEPCGARFGTAIAAVKDLNLDGFNDIVIGAPLEDDHGGAVYIYHGSGKTIRKEYAQRIPSGGDGKTL
  KFFGQSIHGEMDLNGDGLTDVTIGGLGGAALFWSRDVAVVKVTMNFEPNKVNIQKKNCHMEGKETVCINA
  TVCFDVKLKSKEDTIYEADLQYRVTLDSLRQISRSFFSGTQERKVQRNITVRKSECTKHSFYMLDKHDFQ
  DSVRITLDFNLTDPENGPVLDDSLPNSVHEYIPFAKDCGNKEKCISDLSLHVATTEKDLLIVRSQNDKFN
  VSLTVKNTKDSAYNTRTIVHYSPNLVFSGIEAIQKDSCESNHNITCKVGYPFLRRGEMVTFKILFQFNTS
  YLMENVTIYLSATSDSEEPPETLSDNVVNISIPVKYEVGLQFYSSASEYHISIAANETVPEVINSTEDIG
  NEINIFYLIRKSGSFPMPELKLSISFPNMTSNGYPVLYPTGLSSSENANCRPHIFEDPFSINSGKKMTTS
  TDHLKRGTILDCNTCKFATITCNLTSSDISQVNVSLILWKPTFIKSYFSSLNLTIRGELRSENASLVLSS
  SNQKRELAIQISKDGLPGRVPLWVILLSAFAGLLLLMLLILALWKIGFFKRPLKKKMEK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_181501
• ORF Size: 3537 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_181501.2
• RefSeq Size: 4811 bp
• RefSeq ORF: 3540 bp
• Locus ID: 3672
• UniProt ID: P56199
• Cytogenetics: 5q11.2
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Arrhythmogenic right ventricular cardiomyopathy (ARVC), Dilated cardiomyopathy, ECM-receptor interaction, Focal adhesion, Hematopoietic cell lineage, Hypertrophic cardiomyopathy (HCM), Regulation of actin cytoskeleton
• MW: 130.8 kDa
• Gene Summary: This gene encodes the alpha 1 subunit of integrin receptors. This protein heterodimerizes with the beta 1 subunit to form a cell-surface receptor for collagen and laminin. The heterodimeric receptor is involved in cell-cell adhesion and may play a role in inflammation and fibrosis. The alpha 1 subunit contains an inserted (I) von Willebrand factor type I domain which is thought to be involved in collagen binding. [provided by RefSeq, Jul 2008]