MST1 (STK4) (NM_006282) Human Tagged ORF Clone

CAT#: RC210831

STK4 (Myc-DDK-tagged)-Human serine/threonine kinase 4 (STK4)

Product Images

Circular map for RC210831

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  Western blot validation of overexpression lysate (Cat# LY401893) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC210831 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified STK4 protein (Cat# TP310831). The protein was produced from HEK293T cells transfected with STK4 cDNA clone (Cat# RC210831) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: MST1
• Synonyms: KRS2; MST1; YSK3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC210831 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGACGGTACAGCTGAGGAACCCGCCGCGCCGGCAGCTGAAAAAGTTGGATGAAGATAGTTTAACCA
  AACAACCAGAAGAAGTATTTGATGTCTTAGAGAAACTTGGAGAAGGGTCCTATGGCAGCGTATACAAAGC
  TATTCATAAAGAGACCGGCCAGATTGTTGCTATTAAGCAAGTTCCTGTGGAATCAGACCTCCAGGAGATA
  ATCAAAGAAATCTCTATAATGCAGCAATGTGACAGCCCTCATGTAGTCAAATATTATGGCAGTTATTTTA
  AGAACACAGACTTATGGATCGTTATGGAGTACTGTGGGGCTGGTTCTGTATCTGATATCATTCGATTACG
  AAATAAAACGTTAACAGAAGATGAAATAGCTACAATATTACAATCAACTCTTAAGGGACTTGAATACCTT
  CATTTTATGAGAAAAATACACCGAGATATCAAGGCAGGAAATATTTTGCTAAATACAGAAGGACATGCAA
  AACTTGCAGATTTTGGGGTAGCAGGTCAACTTACAGATACCATGGCCAAGCGGAATACAGTGATAGGAAC
  ACCATTTTGGATGGCTCCAGAAGTGATTCAGGAAATTGGATACAACTGTGTAGCAGACATCTGGTCCCTG
  GGAATAACTGCCATAGAAATGGCTGAAGGAAAGCCCCCTTATGCTGATATCCATCCAATGAGGGCAATCT
  TCATGATTCCTACAAATCCTCCTCCCACATTCCGAAAACCAGAGCTATGGTCAGATAACTTTACAGATTT
  TGTGAAACAGTGTCTTGTAAAGAGCCCTGAGCAGAGGGCCACAGCCACTCAGCTCCTGCAGCACCCATTT
  GTCAGGAGTGCCAAAGGAGTGTCAATACTGCGAGACTTAATTAATGAAGCCATGGATGTGAAACTGAAAC
  GCCAGGAATCCCAGCAGCGGGAAGTGGACCAGGACGATGAAGAAAACTCAGAAGAGGATGAAATGGATTC
  TGGCACGATGGTTCGAGCAGTGGGTGATGAGATGGGCACTGTCCGAGTAGCCAGCACCATGACTGATGGA
  GCCAATACTATGATTGAGCACGATGACACGTTGCCATCACAACTGGGCACCATGGTGATCAATGCAGAGG
  ATGAGGAAGAGGAAGGAACTATGAAAAGAAGGGATGAGACCATGCAGCCTGCGAAACCATCCTTTCTTGA
  ATATTTTGAACAAAAAGAAAAGGAAAACCAGATCAACAGCTTTGGCAAGAGTGTACCTGGTCCACTGAAA
  AATTCTTCAGATTGGAAAATACCACAGGATGGAGACTACGAGTTTCTTAAGAGTTGGACAGTGGAGGACC
  TTCAGAAGAGGCTCTTGGCCCTGGACCCCATGATGGAGCAGGAGATTGAAGAGATCCGGCAGAAGTACCA
  GTCCAAGCGGCAGCCCATCCTGGATGCCATAGAGGCTAAGAAGAGACGGCAACAAAACTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC210831 protein sequence
  Red=Cloning site Green=Tags(s)
  
  METVQLRNPPRRQLKKLDEDSLTKQPEEVFDVLEKLGEGSYGSVYKAIHKETGQIVAIKQVPVESDLQEI
  IKEISIMQQCDSPHVVKYYGSYFKNTDLWIVMEYCGAGSVSDIIRLRNKTLTEDEIATILQSTLKGLEYL
  HFMRKIHRDIKAGNILLNTEGHAKLADFGVAGQLTDTMAKRNTVIGTPFWMAPEVIQEIGYNCVADIWSL
  GITAIEMAEGKPPYADIHPMRAIFMIPTNPPPTFRKPELWSDNFTDFVKQCLVKSPEQRATATQLLQHPF
  VRSAKGVSILRDLINEAMDVKLKRQESQQREVDQDDEENSEEDEMDSGTMVRAVGDEMGTVRVASTMTDG
  ANTMIEHDDTLPSQLGTMVINAEDEEEEGTMKRRDETMQPAKPSFLEYFEQKEKENQINSFGKSVPGPLK
  NSSDWKIPQDGDYEFLKSWTVEDLQKRLLALDPMMEQEIEEIRQKYQSKRQPILDAIEAKKRRQQNF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006282
• ORF Size: 1461 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006282.5
• RefSeq Size: 6344 bp
• RefSeq ORF: 1464 bp
• Locus ID: 6789
• UniProt ID: Q13043
• Cytogenetics: 20q13.12
• Domains: pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: MAPK signaling pathway, Non-small cell lung cancer, Pathways in cancer
• MW: 55.6 kDa
• Gene Summary: The protein encoded by this gene is a cytoplasmic kinase that is structurally similar to the yeast Ste20p kinase, which acts upstream of the stress-induced mitogen-activated protein kinase cascade. The encoded protein can phosphorylate myelin basic protein and undergoes autophosphorylation. A caspase-cleaved fragment of the encoded protein has been shown to be capable of phosphorylating histone H2B. The particular phosphorylation catalyzed by this protein has been correlated with apoptosis, and it's possible that this protein induces the chromatin condensation observed in this process. [provided by RefSeq, Jul 2008]