CNDP1 (NM_032649) Human Tagged ORF Clone

CAT#: RC210312

CNDP1 (Myc-DDK-tagged)-Human carnosine dipeptidase 1 (metallopeptidase M20 family) (CNDP1)

Product Images

Circular map for RC210312

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY CNDP1 (Cat# RC210312, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CNDP1(Cat# TA500448). Positive lysates LY403189 (100ug) and LC403189 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY403189) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC210312 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified CNDP1 protein (Cat# TP310312). The protein was produced from HEK293T cells transfected with CNDP1 cDNA clone (Cat# RC210312) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CNDP1
• Synonyms: CN1; CPGL2; HsT2308
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC210312 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATCCCAAACTCGGGAGAATGGCTGCGTCCCTGCTGGCTGTGCTGCTGCTGCTGCTGCTGGAGCGCG
  GCATGTTCTCCTCACCCTCCCCGCCCCCGGCGCTGTTAGAGAAAGTCTTCCAGTACATTGACCTCCATCA
  GGATGAATTTGTGCAGACGCTGAAGGAGTGGGTGGCCATCGAGAGCGACTCTGTCCAGCCTGTGCCTCGC
  TTCAGACAAGAGCTCTTCAGAATGATGGCCGTGGCTGCGGACACGCTGCAGCGCCTGGGGGCCCGTGTGG
  CCTCGGTGGACATGGGTCCTCAGCAGCTGCCCGATGGTCAGAGTCTTCCAATACCTCCCGTCATCCTGGC
  CGAACTGGGGAGCGATCCCACGAAAGGCACCGTGTGCTTCTACGGCCACTTGGACGTGCAGCCTGCTGAC
  CGGGGCGATGGGTGGCTCACGGACCCCTATGTGCTGACGGAGGTAGACGGGAAACTTTATGGACGAGGAG
  CGACCGACAACAAAGGCCCTGTCTTGGCTTGGATCAATGCTGTGAGCGCCTTCAGAGCCCTGGAGCAAGA
  TCTTCCTGTGAATATCAAATTCATCATTGAGGGGATGGAAGAGGCTGGCTCTGTTGCCCTGGAGGAACTT
  GTGGAAAAAGAAAAGGACCGATTCTTCTCTGGTGTGGACTACATTGTAATTTCAGATAACCTGTGGATCA
  GCCAAAGGAAGCCAGCAATCACTTACGGAACCCGGGGGAACAGCTACTTCATGGTGGAGGTGAAATGCAG
  AGACCAGGATTTTCACTCAGGAACCTTTGGTGGCATCCTTAATGAACCAATGGCTGATCTGGTTGCTCTT
  CTCGGTAGCCTGGTAGACTCGTCTGGTCATATCCTGGTCCCTGGAATCTATGATGAAGTGGTTCCTCTTA
  CAGAAGAGGAAATAAATACATACAAAGCCATCCATCTAGACCTAGAAGAATACCGGAATAGCAGCCGGGT
  TGAGAAATTTCTGTTCGATACTAAGGAGGAGATTCTAATGCACCTCTGGAGGTACCCATCTCTTTCTATT
  CATGGGATCGAGGGCGCGTTTGATGAGCCTGGAACTAAAACAGTCATACCTGGCCGAGTTATAGGAAAAT
  TTTCAATCCGTCTAGTCCCTCACATGAATGTGTCTGCGGTGGAAAAACAGGTGACACGACATCTTGAAGA
  TGTGTTCTCCAAAAGAAATAGTTCCAACAAGATGGTTGTTTCCATGACTCTAGGACTACACCCGTGGATT
  GCAAATATTGATGACACCCAGTATCTCGCAGCAAAAAGAGCGATCAGAACAGTGTTTGGAACAGAACCAG
  ATATGATCCGGGATGGATCCACCATTCCAATTGCCAAAATGTTCCAGGAGATCGTCCACAAGAGCGTGGT
  GCTAATTCCGCTGGGAGCTGTTGATGATGGAGAACATTCGCAGAATGAGAAAATCAACAGGTGGAACTAC
  ATAGAGGGAACCAAATTATTTGCTGCCTTTTTCTTAGAGATGGCCCAGCTCCAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC210312 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MDPKLGRMAASLLAVLLLLLLERGMFSSPSPPPALLEKVFQYIDLHQDEFVQTLKEWVAIESDSVQPVPR
  FRQELFRMMAVAADTLQRLGARVASVDMGPQQLPDGQSLPIPPVILAELGSDPTKGTVCFYGHLDVQPAD
  RGDGWLTDPYVLTEVDGKLYGRGATDNKGPVLAWINAVSAFRALEQDLPVNIKFIIEGMEEAGSVALEEL
  VEKEKDRFFSGVDYIVISDNLWISQRKPAITYGTRGNSYFMVEVKCRDQDFHSGTFGGILNEPMADLVAL
  LGSLVDSSGHILVPGIYDEVVPLTEEEINTYKAIHLDLEEYRNSSRVEKFLFDTKEEILMHLWRYPSLSI
  HGIEGAFDEPGTKTVIPGRVIGKFSIRLVPHMNVSAVEKQVTRHLEDVFSKRNSSNKMVVSMTLGLHPWI
  ANIDDTQYLAAKRAIRTVFGTEPDMIRDGSTIPIAKMFQEIVHKSVVLIPLGAVDDGEHSQNEKINRWNY
  IEGTKLFAAFFLEMAQLH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_032649
• ORF Size: 1524 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_032649.6
• RefSeq Size: 2211 bp
• RefSeq ORF: 1524 bp
• Locus ID: 84735
• UniProt ID: Q96KN2
• Cytogenetics: 18q22.3
• Domains: Peptidase_M20
• Protein Families: Protease, Secreted Protein
• Protein Pathways: beta-Alanine metabolism, Histidine metabolism, Metabolic pathways
• MW: 56.8 kDa
• Gene Summary: This gene encodes a member of the M20 metalloprotease family. The encoded protein is specifically expressed in the brain, is a homodimeric dipeptidase which was identified as human carnosinase. This gene contains trinucleotide (CTG) repeat length polymorphism in the coding region. [provided by RefSeq, Jul 2008]