FOXP4 (NM_001012426) Human Tagged ORF Clone

CAT#: RC208611

FOXP4 (Myc-DDK-tagged)-Human forkhead box P4 (FOXP4), transcript variant 1

Product Images

Circular map for RC208611

• 

  Western blot validation of overexpression lysate (Cat# LY422862) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC208611 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified FOXP4 protein (Cat# TP308611). The protein was produced from HEK293T cells transfected with FOXP4 cDNA clone (Cat# RC208611) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: FOXP4
• Synonyms: hFKHLA
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC208611 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGGTGGAATCTGCCTCGGAGACAATCAGGTCGGCTCCATCTGGTCAGAATGGCGTGGGCAGCCTCT
  CTGGGCAAGCAGATGGCAGCAGCGGCGGGGCCACAGGGACAACTGCAAGTGGCACGGGCAGGGAAGTGAC
  CACGGGTGCAGACAGCAATGGTGAGATGAGTCCCGCAGAGCTGCTGCACTTCCAGCAGCAACAGGCTCTC
  CAAGTGGCCCGGCAGTTCCTGCTGCAGCAGGCCTCAGGCCTGAGCTCCCCAGGGAACAATGACAGCAAAC
  AGTCTGCCTCTGCTGTGCAGGTGCCTGTGTCGGTGGCCATGATGTCGCCGCAGATGCTTACCCCGCAACA
  GATGCAGCAGATCCTGTCGCCCCCGCAGCTGCAGGCCTTGCTCCAGCAGCAGCAAGCCCTCATGCTCCAG
  CAGGAGTACTACAAGAAGCAGCAGGAGCAGCTCCACCTGCAGCTCCTCACCCAGCAGCAGGCTGGGAAAC
  CGCAGCCCAAAGAGGCACTGGGGAACAAGCAGCTGGCCTTCCAGCAGCAGCTCCTGCAAATGCAACAGTT
  GCAGCAGCAGCACCTGCTCAACCTGCAGAGGCAGGGGCTGGTCAGCCTGCAGCCCAACCAAGCCTCGGGG
  CCCCTCCAGACCCTTCCGCAAGCAGCTGTTTGCCCAACAGACCTGCCCCAGCTGTGGAAGGGCGAGGGTG
  CCCCCGGGCAGCCTGCCGAGGACAGCGTCAAGCAGGAGGGGCTGGACCTCACTGGCACGGCCGCCACCGC
  TACCTCGTTTGCCGCTCCCCCCAAGGTCTCACCCCCCCTCTCCCACCATACCCTGCCCAACGGACAGCCT
  ACTGTGCTCACATCTCGGAGAGACAGCTCTTCCCACGAGGAGACCCCCGGCTCCCACCCCCTGTACGGAC
  ACGGAGAGTGCAAGTGGCCAGGCTGTGAGACCCTGTGTGAAGACCTGGGCCAGTTTATCAAACACCTCAA
  CACAGAGCACGCCCTGGATGACCGGAGTACAGCCCAGTGCCGGGTACAGATGCAGGTGGTGCAGCAGCTG
  GAGATCCAGCTCGCCAAGGAGAGCGAGCGGCTGCAGGCCATGATGGCCCACCTGCACATGCGGCCCTCGG
  AGCCCAAGCCCTTCAGCCAGCCACTGAACCCGGTCCCCGGCTCCTCCTCATTCTCCAAGGTGACCGTCTC
  TGCAGCAGACTCATTCCCAGATGGTCTCGTGCACCCCCCGACCTCGGCCGCAGCCCCTGTCACCCCTCTA
  CGGCCCCCTGGCCTGGGCTCTGCCTCCCTGCATGGTGGGGGCCCAGCCCGTCGGAGAAGCAGTGACAAGT
  TCTGCTCCCCCATCTCCTCAGAGCTGGCCCAGAATCATGAGTTCTACAAGAACGCCGACGTCCGGCCCCC
  CTTCACCTACGCCTCCCTCATCCGCCAGGCCATCCTGGAAACCCCTGACAGGCAGCTGACCCTGAATGAG
  ATCTATAACTGGTTCACCAGGATGTTCGCCTATTTCCGCAGAAACACTGCCACCTGGAAGAACGCCGTGC
  GCCACAACCTCAGCCTGCACAAGTGCTTCGTCCGCGTGGAGAACGTCAAGGGTGCCGTGTGGACTGTGGA
  CGAGCGGGAGTATCAGAAGCGGAGACCGCCAAAGATGACAGGGAGCCCCACCCTGGTGAAGAACATGATC
  TCTGGCCTCAGCTATGGAGCACTTAATGCCAGCTACCAGGCCGCCCTGGCCGAGAGCAGCTTCCCCCTCC
  TCAACAGCCCTGGCATGCTGAACCCTGGCTCCGCCAGCAGCCTGCTGCCCCTCAGCCACGATGACGTGGG
  TGCCCCCGTGGAGCCGCTGCCCAGCAACGGCAGCAGCAGCCCTCCTCGCCTCTCCCCGCCCCAGTACAGC
  CACCAGGTGCAGGTGAAGGAGGAGCCAGCAGAGGCAGAGGAAGACAGGCAGCCCGGGCCTCCCCTGGGCG
  CCCCTAACCCCAGCGCCTCGGGGCCTCCGGAAGACAGGGACCTGGAGGAGGAGCTGCCGGGAGAAGAACT
  GTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC208611 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MMVESASETIRSAPSGQNGVGSLSGQADGSSGGATGTTASGTGREVTTGADSNGEMSPAELLHFQQQQAL
  QVARQFLLQQASGLSSPGNNDSKQSASAVQVPVSVAMMSPQMLTPQQMQQILSPPQLQALLQQQQALMLQ
  QEYYKKQQEQLHLQLLTQQQAGKPQPKEALGNKQLAFQQQLLQMQQLQQQHLLNLQRQGLVSLQPNQASG
  PLQTLPQAAVCPTDLPQLWKGEGAPGQPAEDSVKQEGLDLTGTAATATSFAAPPKVSPPLSHHTLPNGQP
  TVLTSRRDSSSHEETPGSHPLYGHGECKWPGCETLCEDLGQFIKHLNTEHALDDRSTAQCRVQMQVVQQL
  EIQLAKESERLQAMMAHLHMRPSEPKPFSQPLNPVPGSSSFSKVTVSAADSFPDGLVHPPTSAAAPVTPL
  RPPGLGSASLHGGGPARRRSSDKFCSPISSELAQNHEFYKNADVRPPFTYASLIRQAILETPDRQLTLNE
  IYNWFTRMFAYFRRNTATWKNAVRHNLSLHKCFVRVENVKGAVWTVDEREYQKRRPPKMTGSPTLVKNMI
  SGLSYGALNASYQAALAESSFPLLNSPGMLNPGSASSLLPLSHDDVGAPVEPLPSNGSSSPPRLSPPQYS
  HQVQVKEEPAEAEEDRQPGPPLGAPNPSASGPPEDRDLEEELPGEELS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001012426
• ORF Size: 1359 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001012426.1, NP_001012426.1
• RefSeq Size: 5965 bp
• RefSeq ORF: 2043 bp
• Locus ID: 116113
• UniProt ID: Q8IVH2
• Cytogenetics: 6p21.1
• Protein Families: Transcription Factors
• MW: 73.2 kDa
• Gene Summary: This gene belongs to subfamily P of the forkhead box (FOX) transcription factor family. Forkhead box transcription factors play important roles in the regulation of tissue- and cell type-specific gene transcription during both development and adulthood. Many members of the forkhead box gene family, including members of subfamily P, have roles in mammalian oncogenesis. This gene may play a role in the development of tumors of the kidney and larynx. Alternative splicing of this gene produces multiple transcript variants, some encoding different isoforms. [provided by RefSeq, Jul 2008]