cIAP2 (BIRC3) (NM_001165) Human Tagged ORF Clone

CAT#: RC207764

BIRC3 (Myc-DDK-tagged)-Human baculoviral IAP repeat containing 3 (BIRC3), transcript variant 1

Product Images

Circular map for RC207764

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY BIRC3 (Cat# RC207764, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BIRC3(Cat# TA800063). Positive lysates LY420093 (100ug) and LC420093 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY420093) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC207764 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified BIRC3 protein (Cat# TP307764). The protein was produced from HEK293T cells transfected with BIRC3 cDNA clone (Cat# RC207764) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: cIAP2
• Synonyms: AIP1; API2; c-IAP2; CIAP2; HAIP1; HIAP1; IAP-1; MALT2; MIHC; RNF49
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC207764 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACATAGTAGAAAACAGCATATTCTTATCAAATTTGATGAAAAGCGCCAACACGTTTGAACTGAAAT
  ACGACTTGTCATGTGAACTGTACCGAATGTCTACGTATTCCACTTTTCCTGCTGGGGTTCCTGTCTCAGA
  AAGGAGTCTTGCTCGTGCTGGTTTCTATTACACTGGTGTGAATGACAAGGTCAAATGCTTCTGTTGTGGC
  CTGATGCTGGATAACTGGAAAAGAGGAGACAGTCCTACTGAAAAGCATAAAAAGTTGTATCCTAGCTGCA
  GATTCGTTCAGAGTCTAAATTCCGTTAACAACTTGGAAGCTACCTCTCAGCCTACTTTTCCTTCTTCAGT
  AACAAATTCCACACACTCATTACTTCCGGGTACAGAAAACAGTGGATATTTCCGTGGCTCTTATTCAAAC
  TCTCCATCAAATCCTGTAAACTCCAGAGCAAATCAAGATTTTTCTGCCTTGATGAGAAGTTCCTACCACT
  GTGCAATGAATAACGAAAATGCCAGATTACTTACTTTTCAGACATGGCCATTGACTTTTCTGTCGCCAAC
  AGATCTGGCAAAAGCAGGCTTTTACTACATAGGACCTGGAGACAGAGTGGCTTGCTTTGCCTGTGGTGGA
  AAATTGAGCAATTGGGAACCGAAGGATAATGCTATGTCAGAACACCTGAGACATTTTCCCAAATGCCCAT
  TTATAGAAAATCAGCTTCAAGACACTTCAAGATACACAGTTTCTAATCTGAGCATGCAGACACATGCAGC
  CCGCTTTAAAACATTCTTTAACTGGCCCTCTAGTGTTCTAGTTAATCCTGAGCAGCTTGCAAGTGCGGGT
  TTTTATTATGTGGGTAACAGTGATGATGTCAAATGCTTTTGCTGTGATGGTGGACTCAGGTGTTGGGAAT
  CTGGAGATGATCCATGGGTTCAACATGCCAAGTGGTTTCCAAGGTGTGAGTACTTGATAAGAATTAAAGG
  ACAGGAGTTCATCCGTCAAGTTCAAGCCAGTTACCCTCATCTACTTGAACAGCTGCTATCCACATCAGAC
  AGCCCAGGAGATGAAAATGCAGAGTCATCAATTATCCATTTTGAACCTGGAGAAGACCATTCAGAAGATG
  CAATCATGATGAATACTCCTGTGATTAATGCTGCCGTGGAAATGGGCTTTAGTAGAAGCCTGGTAAAACA
  GACAGTTCAGAGAAAAATCCTAGCAACTGGAGAGAATTATAGACTAGTCAATGATCTTGTGTTAGACTTA
  CTCAATGCAGAAGATGAAATAAGGGAAGAGGAGAGAGAAAGAGCAACTGAGGAAAAAGAATCAAATGATT
  TATTATTAATCCGGAAGAATAGAATGGCACTTTTTCAACATTTGACTTGTGTAATTCCAATCCTGGATAG
  TCTACTAACTGCCGGAATTATTAATGAACAAGAACATGATGTTATTAAACAGAAGACACAGACGTCTTTA
  CAAGCAAGAGAACTGATTGATACGATTTTAGTAAAAGGAAATATTGCAGCCACTGTATTCAGAAACTCTC
  TGCAAGAAGCTGAAGCTGTGTTATATGAGCATTTATTTGTGCAACAGGACATAAAATATATTCCCACAGA
  AGATGTTTCAGATCTACCAGTGGAAGAACAATTGCGGAGACTACAAGAAGAAAGAACATGTAAAGTGTGT
  ATGGACAAAGAAGTGTCCATAGTGTTTATTCCTTGTGGTCATCTAGTAGTATGCAAAGATTGTGCTCCTT
  CTTTAAGAAAGTGTCCTATTTGTAGGAGTACAATCAAGGGTACAGTTCGTACATTTCTTTCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC207764 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MNIVENSIFLSNLMKSANTFELKYDLSCELYRMSTYSTFPAGVPVSERSLARAGFYYTGVNDKVKCFCCG
  LMLDNWKRGDSPTEKHKKLYPSCRFVQSLNSVNNLEATSQPTFPSSVTNSTHSLLPGTENSGYFRGSYSN
  SPSNPVNSRANQDFSALMRSSYHCAMNNENARLLTFQTWPLTFLSPTDLAKAGFYYIGPGDRVACFACGG
  KLSNWEPKDNAMSEHLRHFPKCPFIENQLQDTSRYTVSNLSMQTHAARFKTFFNWPSSVLVNPEQLASAG
  FYYVGNSDDVKCFCCDGGLRCWESGDDPWVQHAKWFPRCEYLIRIKGQEFIRQVQASYPHLLEQLLSTSD
  SPGDENAESSIIHFEPGEDHSEDAIMMNTPVINAAVEMGFSRSLVKQTVQRKILATGENYRLVNDLVLDL
  LNAEDEIREEERERATEEKESNDLLLIRKNRMALFQHLTCVIPILDSLLTAGIINEQEHDVIKQKTQTSL
  QARELIDTILVKGNIAATVFRNSLQEAEAVLYEHLFVQQDIKYIPTEDVSDLPVEEQLRRLQEERTCKVC
  MDKEVSIVFIPCGHLVVCKDCAPSLRKCPICRSTIKGTVRTFLS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001165
• ORF Size: 1812 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001165.5
• RefSeq Size: 6932 bp
• RefSeq ORF: 1815 bp
• Locus ID: 330
• UniProt ID: Q13489
• Cytogenetics: 11q22.2
• Domains: CARD, BIR, RING
• Protein Families: Druggable Genome
• Protein Pathways: Apoptosis, Focal adhesion, NOD-like receptor signaling pathway, Pathways in cancer, Small cell lung cancer, Ubiquitin mediated proteolysis
• MW: 68.4 kDa
• Gene Summary: This gene encodes a member of the IAP family of proteins that inhibit apoptosis by binding to tumor necrosis factor receptor-associated factors TRAF1 and TRAF2, probably by interfering with activation of ICE-like proteases. The encoded protein inhibits apoptosis induced by serum deprivation but does not affect apoptosis resulting from exposure to menadione, a potent inducer of free radicals. It contains 3 baculovirus IAP repeats and a ring finger domain. Transcript variants encoding the same isoform have been identified. [provided by RefSeq, Aug 2011]