Dishevelled 3 (DVL3) (NM_004423) Human Tagged ORF Clone

CAT#: RC207100

DVL3 (Myc-DDK-tagged)-Human dishevelled, dsh homolog 3 (Drosophila) (DVL3)

Product Images

Circular map for RC207100

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Dishevelled 3
• Synonyms: DRS3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC207100 representing NM_004423
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCGAGACCAAGATCATCTACCACTTGGATGGGCAGGAGACGCCGTACCTTGTGAAGCTGCCCCTGC
  CCGCCGAGCGCGTCACCTTGGCGGACTTTAAGGGCGTTTTGCAGCGACCCAGCTATAAGTTCTTCTTCAA
  GTCTATGGACGACGATTTCGGAGTGGTGAAGGAGGAGATCTCGGATGACAATGCCAAGCTACCATGCTTC
  AATGGCCGGGTGGTGTCCTGGCTGGTGTCAGCTGAGGGCTCACACCCAGACCCAGCCCCCTTCTGTGCTG
  ATAACCCATCGGAGCTGCCACCACCTATGGAGCGCACGGGAGGCATCGGGGACTCCCGACCCCCATCCTT
  CCACCCTCATGCTGGTGGGGGCAGCCAGGAGAACCTGGACAATGACACAGAGACGGACTCTTTGGTGTCT
  GCCCAGCGAGAGCGGCCACGCCGGAGGGATGGCCCAGAGCATGCAACCCGGCTAAATGGAACTGCGAAGG
  GGGAACGGCGGCGAGAACCAGGGGGTTATGATAGCTCATCCACCCTTATGAGCAGTGAGCTGGAGACCAC
  CAGCTTCTTTGACTCAGATGAGGATGACTCCACCAGCAGGTTCAGCAGCTCCACAGAACAGAGCAGTGCC
  TCACGCCTGATGAGAAGACACAAGCGGCGGCGGCGGAAGCAGAAGGTTTCTCGGATTGAGCGGTCCTCGT
  CCTTCAGCAGCATCACGGACTCCACCATGTCACTCAACATCATCACGGTCACTCTCAACATGGAAAAATA
  TAACTTCTTGGGCATCTCCATTGTGGGCCAAAGCAACGAGCGTGGTGACGGCGGCATCTACATTGGCTCT
  ATCATGAAGGGTGGGGCCGTGGCTGCTGATGGACGCATCGAGCCAGGAGATATGTTGTTACAGGTAAACG
  AGATCAACTTTGAGAACATGAGTAATGACGATGCAGTCCGGGTACTGCGGGAGATTGTGCACAAACCGGG
  GCCCATCACCCTGACTGTAGCCAAGTGCTGGGACCCAAGTCCACGTGGTTGCTTCACATTGCCCAGGAGC
  GAGCCCATCCGGCCCATTGACCCTGCGGCCTGGGTCTCCCACACTGCAGCCATGACCGGCACCTTCCCTG
  CATACGGCATGAGCCCCTCCCTGAGCACCATCACCTCCACCAGCTCCTCCATCACCAGTTCCATCCCTGA
  CACAGAGCGCCTAGACGACTTCCACTTGTCCATCCACAGTGACATGGCTGCCATCGTAAAAGCCATGGCC
  TCCCCTGAATCAGGGTTGGAGGTCCGTGACCGCATGTGGCTCAAGATTACCATCCCTAATGCTTTCATCG
  GCTCAGATGTGGTGGACTGGCTGTACCACAATGTGGAAGGCTTCACGGACCGGAGGGAGGCCCGCAAGTA
  TGCCAGCAACCTGCTGAAAGCTGGCTTCATCCGCCATACCGTCAACAAGATCACCTTCTCCGAGCAGTGC
  TACTACATCTTCGGTGACCTCTGCGGCAACATGGCCAACCTGTCTCTCCACGATCACGATGGCTCCAGTG
  GCGCCTCTGACCAGGACACACTGGCCCCTTTGCCGCACCCGGGGGCCGCCCCTTGGCCCATGGCTTTCCC
  GTACCAGTACCCGCCACCCCCGCACCCATACAACCCGCACCCGGGCTTCCCGGAGCTGGGCTACAGCTAC
  GGCGGGGGCAGCGCCAGCAGTCAGCACAGCGAAGGCAGTCGGAGCAGTGGCTCCAACCGTAGCGGCAGCG
  ATCGGAGGAAGGAGAAGGACCCGAAGGCCGGGGACTCCAAGTCCGGGGGCAGCGGCAGCGAATCGGACCA
  CACCACACGCAGCAGCCTGCGGGGGCCGCGGGAGCGGGCGCCCAGCGAGCGCTCAGGGCCGGCGGCCAGC
  GAGCACAGCCACCGCAGCCACCATTCCCTGGCCAGCAGCCTTCGCAGCCACCACACACACCCGAGCTACG
  GTCCTCCCGGAGTGCCCCCTCTCTACGGCCCCCCCATGCTGATGATGCCCCCGCCGCCCGCGGCCATGGG
  GCCCCCAGGAGCCCCTCCGGGCCGCGACCTGGCCTCAGTGCCCCCGGAACTGACCGCCAGCAGACAGTCC
  TTCCGCATGGCCATGGGAAACCCCAGTGAGTTCTTTGTGGATGTGATG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC207100 representing NM_004423
  Red=Cloning site Green=Tags(s)
  
  MGETKIIYHLDGQETPYLVKLPLPAERVTLADFKGVLQRPSYKFFFKSMDDDFGVVKEEISDDNAKLPCF
  NGRVVSWLVSAEGSHPDPAPFCADNPSELPPPMERTGGIGDSRPPSFHPHAGGGSQENLDNDTETDSLVS
  AQRERPRRRDGPEHATRLNGTAKGERRREPGGYDSSSTLMSSELETTSFFDSDEDDSTSRFSSSTEQSSA
  SRLMRRHKRRRRKQKVSRIERSSSFSSITDSTMSLNIITVTLNMEKYNFLGISIVGQSNERGDGGIYIGS
  IMKGGAVAADGRIEPGDMLLQVNEINFENMSNDDAVRVLREIVHKPGPITLTVAKCWDPSPRGCFTLPRS
  EPIRPIDPAAWVSHTAAMTGTFPAYGMSPSLSTITSTSSSITSSIPDTERLDDFHLSIHSDMAAIVKAMA
  SPESGLEVRDRMWLKITIPNAFIGSDVVDWLYHNVEGFTDRREARKYASNLLKAGFIRHTVNKITFSEQC
  YYIFGDLCGNMANLSLHDHDGSSGASDQDTLAPLPHPGAAPWPMAFPYQYPPPPHPYNPHPGFPELGYSY
  GGGSASSQHSEGSRSSGSNRSGSDRRKEKDPKAGDSKSGGSGSESDHTTRSSLRGPRERAPSERSGPAAS
  EHSHRSHHSLASSLRSHHTHPSYGPPGVPPLYGPPMLMMPPPPAAMGPPGAPPGRDLASVPPELTASRQS
  FRMAMGNPSEFFVDVM
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004423
• ORF Size: 2148 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004423.4
• RefSeq Size: 5062 bp
• RefSeq ORF: 2151 bp
• Locus ID: 1857
• UniProt ID: Q92997
• Cytogenetics: 3q27.1
• Domains: DEP, DAX, PDZ, Dishevelled
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS
• Protein Pathways: Basal cell carcinoma, Colorectal cancer, Melanogenesis, Notch signaling pathway, Pathways in cancer, Wnt signaling pathway
• MW: 77.9 kDa
• Gene Summary: This gene is a member of a multi-gene family which shares strong similarity with the Drosophila dishevelled gene, dsh. The Drosophila dishevelled gene encodes a cytoplasmic phosphoprotein that regulates cell proliferation. [provided by RefSeq, Jul 2008]