BAT5 (ABHD16A) (NM_021160) Human Tagged ORF Clone

CAT#: RC205122

ABHD16A (Myc-DDK-tagged)-Human abhydrolase domain containing 16A (ABHD16A), transcript variant 1

Product Images

Circular map for RC205122

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: BAT5
• Synonyms: BAT5; D6S82E; hBAT5; NG26; PP199
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC205122 representing NM_021160
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGAAGCTGCTGAGCTGCGTCCTAGGCCCCCGGCTCTACAAAATCTACCGGGAGAGGGACTCTGAAA
  GGGCCCCGGCCAGCGTCCCTGAGACGCCAACGGCAGTCACTGCCCCCCATTCCAGCTCCTGGGATACGTA
  CTATCAGCCCCGTGCCCTGGAGAAACATGCTGACAGCATCCTGGCACTGGCTTCAGTATTCTGGTCCATC
  TCTTATTACTCCTCTCCCTTCGCCTTCTTCTACTTGTACAGGAAAGGTTACTTGAGTTTGTCCAAAGTGG
  TGCCGTTTTCTCACTATGCTGGGACATTGCTGCTACTTCTGGCAGGTGTGGCCTGCCTCCGAGGCATTGG
  CCGCTGGACCAACCCCCAGTACCGGCAGTTCATCACCATCTTGGAAGCAACACATCGGAACCAGTCTTCA
  GAAAACAAGAGGCAGCTTGCCAACTACAACTTTGACTTCCGGAGCTGGCCAGTCGACTTCCACTGGGAAG
  AACCCAGCAGCCGGAAGGAGTCTCGAGGGGGCCCTTCCCGCCGGGGTGTGGCCCTGCTTCGCCCAGAGCC
  CCTGCACCGGGGGACAGCAGACACCCTCCTCAACCGGGTTAAGAAGCTGCCTTGTCAGATCACCAGCTAC
  CTGGTGGCGCACACCCTAGGGCGCCGGATGCTGTATCCAGGCTCTGTGTACCTGCTGCAGAAGGCCCTCA
  TGCCTGTGCTGCTGCAGGGCCAGGCCCGACTGGTGGAAGAGTGTAATGGGCGCCGGGCAAAGCTGCTGGC
  CTGTGATGGCAATGAGATTGACACCATGTTTGTGGACCGGCGGGGGACAGCTGAGCCCCAGGGACAGAAG
  CTGGTGATCTGCTGTGAGGGGAATGCTGGGTTTTATGAGGTGGGCTGCGTCTCCACGCCCCTGGAAGCTG
  GATATTCAGTCCTGGGCTGGAATCATCCAGGCTTTGCTGGAAGCACGGGGGTGCCATTCCCGCAGAATGA
  GGCTAATGCCATGGATGTGGTGGTCCAGTTTGCCATCCACCGCCTAGGCTTCCAGCCCCAGGACATCATC
  ATCTACGCCTGGTCCATCGGCGGCTTCACTGCCACGTGGGCAGCCATGTCCTACCCAGATGTTAGTGCCA
  TGATCCTGGATGCCTCCTTTGATGACCTGGTGCCCTTGGCCTTGAAGGTCATGCCAGACAGCTGGAGGGG
  CCTGGTGACCAGGACCGTGAGGCAGCATCTCAATCTAAACAACGCGGAGCAGCTGTGCAGATACCAGGGT
  CCTGTACTGCTGATCCGGAGAACCAAGGATGAGATCATCACCACCACGGTTCCTGAGGACATCATGTCCA
  ACCGAGGCAATGACCTCCTGCTGAAGCTCCTGCAGCATCGGTATCCCCGGGTGATGGCAGAGGAGGGTCT
  TCGAGTGGTGAGGCAGTGGTTGGAGGCCTCCTCACAGCTGGAGGAAGCCTCAATTTATAGCCGATGGGAG
  GTGGAAGAGGACTGGTGTCTGTCTGTCCTCCGCTCCTACCAGGCAGAACACGGGCCCGACTTCCCCTGGA
  GCGTGGGGGAGGACATGAGTGCAGATGGACGGCGGCAGCTGGCTTTGTTTCTGGCTCGGAAGCATCTGCA
  CAACTTTGAGGCCACTCACTGCACCCCACTCCCAGCCCAGAACTTCCAGATGCCCTGGCACCTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC205122 representing NM_021160
  Red=Cloning site Green=Tags(s)
  
  MAKLLSCVLGPRLYKIYRERDSERAPASVPETPTAVTAPHSSSWDTYYQPRALEKHADSILALASVFWSI
  SYYSSPFAFFYLYRKGYLSLSKVVPFSHYAGTLLLLLAGVACLRGIGRWTNPQYRQFITILEATHRNQSS
  ENKRQLANYNFDFRSWPVDFHWEEPSSRKESRGGPSRRGVALLRPEPLHRGTADTLLNRVKKLPCQITSY
  LVAHTLGRRMLYPGSVYLLQKALMPVLLQGQARLVEECNGRRAKLLACDGNEIDTMFVDRRGTAEPQGQK
  LVICCEGNAGFYEVGCVSTPLEAGYSVLGWNHPGFAGSTGVPFPQNEANAMDVVVQFAIHRLGFQPQDII
  IYAWSIGGFTATWAAMSYPDVSAMILDASFDDLVPLALKVMPDSWRGLVTRTVRQHLNLNNAEQLCRYQG
  PVLLIRRTKDEIITTTVPEDIMSNRGNDLLLKLLQHRYPRVMAEEGLRVVRQWLEASSQLEEASIYSRWE
  VEEDWCLSVLRSYQAEHGPDFPWSVGEDMSADGRRQLALFLARKHLHNFEATHCTPLPAQNFQMPWHL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_021160
• ORF Size: 1674 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021160.3
• RefSeq Size: 2019 bp
• RefSeq ORF: 1677 bp
• Locus ID: 7920
• UniProt ID: O95870
• Cytogenetics: 6p21.33
• Protein Families: Transmembrane
• MW: 63.2 kDa
• Gene Summary: A cluster of genes, BAT1-BAT5, has been localized in the vicinity of the genes for tumor necrosis factor alpha and tumor necrosis factor beta. These genes are all within the human major histocompatibility complex class III region. The protein encoded by this gene is thought to be involved in some aspects of immunity. Alternatively spliced transcript variants have been described. [provided by RefSeq, Apr 2010]