BRAF35 (HMG20B) (NM_006339) Human Tagged ORF Clone

CAT#: RC202444

HMG20B (Myc-DDK-tagged)-Human high mobility group 20B (HMG20B)

Product Images

Circular map for RC202444

• 

  Western blot validation of overexpression lysate (Cat# LY401908) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC202444 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: BRAF35
• Synonyms: BRAF25; BRAF35; HMGX2; HMGXB2; PP7706; pp8857; SMARCE1r; SOXL
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC202444 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCCACGGCCCCAAGCAGCCCGGCGCGGCCGCCGCGCCGGCGGGCGGCAAGGCTCCGGGCCAGCATG
  GGGGCTTCGTGGTGACTGTCAAGCAAGAGCGCGGCGAGGGTCCACGCGCGGGCGAGAAGGGGTCCCACGA
  GGAGGAGCCGGTGAAGAAACGCGGCTGGCCCAAGGGCAAGAAGCGGAAGAAGATTCTGCCGAATGGGCCC
  AAGGCACCGGTCACGGGCTACGTGCGCTTCCTGAACGAGCGGCGCGAGCAGATCCGCACGCGCCACCCGG
  ATCTGCCCTTTCCCGAGATCACCAAGATGCTGGGCGCCGAGTGGAGCAAGCTGCAGCCAACGGAAAAGCA
  GCGGTACCTGGATGAGGCCGAGAGAGAGAAGCAGCAGTACATGAAGGAGCTGCGGGCGTACCAGCAGTCT
  GAAGCCTATAAGATGTGCACGGAGAAGATCCAGGAGAAGAAGATCAAGAAAGAAGACTCGAGCTCTGGGC
  TCATGAACACTCTCCTGAATGGACACAAGGGTGGGGACTGCGATGGCTTCTCCACCTTCGATGTTCCCAT
  CTTCACTGAAGAGTTCTTGGACCAAAACAAAGCGCGTGAGGCGGAGCTTCGGCGCTTGCGGAAGATGAAT
  GTGGCCTTCGAGGAGCAGAACGCGGTACTGCAGAGGCACACGCAGAGCATGAGCAGCGCGCGCGAGCGTC
  TGGAGCAGGAGCTGGCGCTGGAGGAGCGGAGGACGCTGGCGCTGCAGCAGCAGCTCCAGGCCGTGCGCCA
  GGCGCTCACCGCCAGCTTCGCCTCACTGCCGGTGCCGGGCACGGGCGAAACGCCCACGCTGGGCACTCTG
  GACTTCTACATGGCCCGGCTTCACGGAGCCATCGAGCGCGACCCCGCCCAGCACGAGAAGCTCATCGTCC
  GCATCAAGGAAATCCTGGCCCAGGTCGCCAGCGAGCACCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC202444 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSHGPKQPGAAAAPAGGKAPGQHGGFVVTVKQERGEGPRAGEKGSHEEEPVKKRGWPKGKKRKKILPNGP
  KAPVTGYVRFLNERREQIRTRHPDLPFPEITKMLGAEWSKLQPTEKQRYLDEAEREKQQYMKELRAYQQS
  EAYKMCTEKIQEKKIKKEDSSSGLMNTLLNGHKGGDCDGFSTFDVPIFTEEFLDQNKAREAELRRLRKMN
  VAFEEQNAVLQRHTQSMSSARERLEQELALEERRTLALQQQLQAVRQALTASFASLPVPGTGETPTLGTL
  DFYMARLHGAIERDPAQHEKLIVRIKEILAQVASEHL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006339
• ORF Size: 951 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006339.3
• RefSeq Size: 1584 bp
• RefSeq ORF: 954 bp
• Locus ID: 10362
• UniProt ID: Q9P0W2
• Cytogenetics: 19p13.3
• Protein Families: Druggable Genome, Transcription Factors
• MW: 35.8 kDa
• Gene Summary: Required for correct progression through G2 phase of the cell cycle and entry into mitosis. Required for RCOR1/CoREST mediated repression of neuronal specific gene promoters.[UniProtKB/Swiss-Prot Function]