Per3 (NM_001289878) Mouse Tagged ORF Clone

CAT#: MR231176

Per3 (myc-DDK-tagged) - Mouse period circadian clock 3 (Per3), transcript variant 3

Product Images

Circular map for MR231176

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Per3
• Synonyms: 2810049O06Rik; mPer3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR231176 representing NM_001289878
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTTGCCATACACCAAAAAGTTTTAAAGTATGCCGGCCACCCTCCGTTTGAACACTCGCCCGTCAGAT
  TCTGCACTCAGAACGGAGAGTATGTCATTCTGGATTCCAGCTGGTCCAGCTTTGTCAACCCCTGGAGCCG
  GAAGGTCTCCTTCATCATTGGTCGACATAAAGTCCGAACGAGTCCATTAAATGAAGATGTTTTTGCCACC
  AGAATAAAAAAGGCAGCCAGTAACGACAAAGACATAGCAGAATTACAAGAACAAATTCACAAACTTCTCT
  TGCAGCCGGTTCATGCTAGTGCTTCCAGTGGCTACGGGAGCCTGGGCAGCAGCGGCTCACAGGAGCAGCA
  CGTCAGCATCACCTCTTCGAGTGAGTCCAGCGGGCACTGTCCGGAGGAAGGCCAGCATGAGCAGATGACC
  CTGCAGCAGGTCTATGCCAGTGTAAACAAAATTAAGAATGTGGGCCAACAGCTCTACATCGAGTCCATGG
  CCAGATCATCAGTGAAGCCAGTGGCAGAGACGTGCGTGGAACCGCAGGGTGGTGATGAGCAGAAGGACTT
  TTCTTCCTCTCAGACACTGAAAAATAAAAGCACCACGGATACTGGCTCCGGTGGCAATCTGCAGCAAGAG
  CAGCCCAGCTCGTCCTATCAGCAGATGAACTGTATCGACAGTGTCATCAGGTACCTGACAAGCTACAGCC
  TCCCGGCCTTGAAAAGAAAGTGCATCTCCTGCACAAACACATCTTCATCCTCAGAAGAAGCCAAGCCAAT
  CCCGGAGGTGGACAGCAGCCAGAGAGACACGGAACAGCTCCTGGACATACGGAAACAGGAAACAACTGGA
  CCATCCACAGACATCGAAGGAGGTGCTGCTCGGACCCTGTCCACCGCCGCACTGAGCGTGGCGTCTGGCA
  TCAGCCAGTGCAGCTGCAGCAGCACCTCTGGCCACGCTCCGCCCCTACAGTCAGCAGAAAGTGTTGCCGT
  GGCGTGTAAGCCGTGGGCCCTGAGAACGAAGGCCTCTCACCTGGCTGCAGGAGGATTTAAGCACGTGGGG
  CTCACAGCAGCTGTCCTCTCTGCACACACACAGAAGGAAGAGCAGAACTACGTTGACAGGTTCCGGGAAA
  AGATCCTGACCTCGCCCTACGGTTGCTATCTTCAGCAAGAGAGCAGAAACCGTGCTCAGTACTCCTGTGT
  TCAAGCAGGGTCCACTGCTAAGCACAGCAGATGTGCTGGAAGCGAGAGGCAGAAGCACAAACGAAAGAAG
  TTGCCAGCACCTGTGGACACCAGCAGCCCCGGTGCCCACCTCTGTCCCCATGTCACAGGACTCCTCCCGG
  ATGAGCAGCACTGGGGCCCATCCGCTAGCCCCTCCCCCCTCGGCGCAGGCTTAGCATTCCCCTCGGCCCT
  GGTAGTTCCCAGCCAGACCCCTTATCTCCTCCCCTCTTTTCCCCTCCAAGATATGGCCTCTCAGGGAGTG
  GGGGTCTCGGCAGCCTGGGGAGCTGCAGCCGGATGTCCACCTCTGTCCGCCGGCCCCCAGGCTGTTGCCG
  CGTTCCCCTCCGCTTACGTGGATACTTTGATGACCATCTTCCTGCACAACGCCCCTCTCTTCCCTCTGTG
  GCCGCCCTCGTTCTCCCCATACCCATCCCTGGGGGCCGCAGGGTCTTCTGAACTGGCACCCTTAGTACCA
  GCAATGGCTCCAAACCCGGAACCAACCACTTCAGGCCACAGCCAAAGGAGAGTGGAGGAGAACTGGGAGG
  CACACAGTGAAGAGCTTCCGTTCATTAGCTCACGGAGCAGTTCACCGTTACAGTTAAATTTACTCCAGGA
  AGAAATGCCTGCGCCGTCAGAGTCCGCAGACGCAGTGAGAAGAGGCGCTGGGCCAGACGCTAAGCATCAC
  TGTGTTACAGGTCCCAGTGGCAGTAGGAGCCGTCACTGCACCTCTGGTGAGCTGGCCACGGCAACAGCGC
  AGCAGGAGTCTGCTGCTGCCTCAGGAAGCAGTGCCAGCAGTATATACTTCAGTAGCACTGACTATGCTTC
  TGAAGTCTCTGAAAACAGACAGAGGCCACAGGATAGACAGAGAGACGAAGCCCTTCCCGGGGCGGCTGAA
  GAGTCCATCTGGAGAATGATAGAGCGGACACCAGAGTGTGTTCTCATGACATACCAGGTGCCCGAGAGGG
  GTCGAGAGGAGGTGCTGAAGCAGGACCTGGAGAAGCTCCAGAGCATGGAACAGCAGCAGCCCCTGTTCTC
  TCCCGCGCAGAGGGAGGAGCTGGCCAAGGTGCGCTCCTGGATCCACAGCCACACAGCCCCTCAGGAGGGA
  CACCTCCAGAGCTGTGTCGCCTGTGAAGACAGAGGTTCAGTGGGTGACACTGCAGAGGTCCTGGAACAGC
  ACCCAGCAGAAGACACCAGT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR231176 representing NM_001289878
  Red=Cloning site Green=Tags(s)
  
  MVAIHQKVLKYAGHPPFEHSPVRFCTQNGEYVILDSSWSSFVNPWSRKVSFIIGRHKVRTSPLNEDVFAT
  RIKKAASNDKDIAELQEQIHKLLLQPVHASASSGYGSLGSSGSQEQHVSITSSSESSGHCPEEGQHEQMT
  LQQVYASVNKIKNVGQQLYIESMARSSVKPVAETCVEPQGGDEQKDFSSSQTLKNKSTTDTGSGGNLQQE
  QPSSSYQQMNCIDSVIRYLTSYSLPALKRKCISCTNTSSSSEEAKPIPEVDSSQRDTEQLLDIRKQETTG
  PSTDIEGGAARTLSTAALSVASGISQCSCSSTSGHAPPLQSAESVAVACKPWALRTKASHLAAGGFKHVG
  LTAAVLSAHTQKEEQNYVDRFREKILTSPYGCYLQQESRNRAQYSCVQAGSTAKHSRCAGSERQKHKRKK
  LPAPVDTSSPGAHLCPHVTGLLPDEQHWGPSASPSPLGAGLAFPSALVVPSQTPYLLPSFPLQDMASQGV
  GVSAAWGAAAGCPPLSAGPQAVAAFPSAYVDTLMTIFLHNAPLFPLWPPSFSPYPSLGAAGSSELAPLVP
  AMAPNPEPTTSGHSQRRVEENWEAHSEELPFISSRSSSPLQLNLLQEEMPAPSESADAVRRGAGPDAKHH
  CVTGPSGSRSRHCTSGELATATAQQESAAASGSSASSIYFSSTDYASEVSENRQRPQDRQRDEALPGAAE
  ESIWRMIERTPECVLMTYQVPERGREEVLKQDLEKLQSMEQQQPLFSPAQREELAKVRSWIHSHTAPQEG
  HLQSCVACEDRGSVGDTAEVLEQHPAEDTS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001289878
• ORF Size: 2400 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001289878.1, NP_001276807.1
• RefSeq Size: 6048 bp
• RefSeq ORF: 2403 bp
• Locus ID: 18628
• Cytogenetics: 4 E2
• MW: 86.6 kDa
• Gene Summary: This gene is a member of the Period family of genes and is expressed in a circadian pattern in the suprachiasmatic nucleus, the primary circadian pacemaker in the mammalian brain. Genes in this family encode components of the circadian rhythms of locomotor activity, metabolism, and behavior. This gene is upregulated by Clock/Arntl heterodimers but then represses this upregulation in a feedback loop using Per/Cry heterodimers to interact with Clock/Arntl. Polymorphisms in this gene have been linked to sleep disorders. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2014]