Rassf1 (NM_001243748) Mouse Tagged ORF Clone

CAT#: MR229341

Rassf1 (myc-DDK-tagged) - Mouse Ras association (RalGDS/AF-6) domain family member 1 (Rassf1), transcript variant 1

Product Images

Circular map for MR229341

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Rassf1
• Synonyms: 123F2; AA536941; AU044980; NORE2A; Rassf1A; Rassf1B; Rassf1C; RDA32; REH3P21
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR229341 representing NM_001243748
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGGCGGAGCCAGAACTCATTGAACTACGCGAGCTGGCACCGTCCGGGCGCATCGGTCCAGGCCGTA
  CCCGGCTGGAGCGTGCCAACGCTCTGCGCATCGCCCCGGGTACAACACGCAATCCGTCACAGCAGCACGT
  CCCGGGTCGTGGCCACCGTTTCCAGCCTGCAGGGCCCACCACGCACACGTGGTGCGACCTCTGTGGAGAC
  TTCATCTGGGGCGTCGTGCGCAAGGGCCTACAGTGCGCGCACTGCAAGTTCACCTGCCATTACCGTTGTC
  GCGCGCTCGTCTGCTTGGACTGCTGCGGGCCCCGAGACCTGGGCTGGGACTCTGCACTAGAGCGGGATAC
  GAACGTGGATGAGGCTGTAGAGCGGGAGACACCCGATCTTTCTCAAGCTGAGACTGAGCAGAAAATCAAG
  GACTACAATGGCCAGATCAACAGCAACCTCTTCATGAGCCTGAATAAGGATGGCTCCTACACAGGCTTCA
  TCAAGGTTCAGCTGAAACTAGTGCGTCCTGTTTCAGTGCCTTCCAGCAAGAAACCACCTTCCTTACAGGA
  CGCCCGGAGAGGTACGGGGCGGAGCACAGCCGTGAAGCGCCGCACCTCTTTTTACTTGCCTAAGGATGCT
  ATTAAGCATCTGCATGTTCTATCACGAACACGGGCACGTGAGGTCATTGAGGCCCTGCTTCGAAAATTCA
  TGGTCGTAGATGATCCTCGCAAGTTTGCACTCTTTGAGCGAACTGAACGGCATGGCCAAGTATACCTCCG
  GAAGCTGTCGGATGACGAGCAGCCCTTGAAGCTGCGGCTTCTTGCAGGGCCCAGTGAAAAAGCCCTGAGC
  TTTGTCCTGAAGGAAAATGACTCGGGAGAGGTGAACTGGGATGCCTTCAGCATGCCTGAACTGCACAATT
  TCCTACGAATCCTGCAGCGGGAAGAAGAGGAACACCTTCGCCAGATCCTGCAGAAGTATTCTCGTTGTCG
  CCAGAAGATCCAGGAGGCTCTGCACGCCTGTCCTTTGGGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR229341 representing NM_001243748
  Red=Cloning site Green=Tags(s)
  
  MSAEPELIELRELAPSGRIGPGRTRLERANALRIAPGTTRNPSQQHVPGRGHRFQPAGPTTHTWCDLCGD
  FIWGVVRKGLQCAHCKFTCHYRCRALVCLDCCGPRDLGWDSALERDTNVDEAVERETPDLSQAETEQKIK
  DYNGQINSNLFMSLNKDGSYTGFIKVQLKLVRPVSVPSSKKPPSLQDARRGTGRSTAVKRRTSFYLPKDA
  IKHLHVLSRTRAREVIEALLRKFMVVDDPRKFALFERTERHGQVYLRKLSDDEQPLKLRLLAGPSEKALS
  FVLKENDSGEVNWDAFSMPELHNFLRILQREEEEHLRQILQKYSRCRQKIQEALHACPLG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001243748
• ORF Size: 1020 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001243748.1, NP_001230677.1
• RefSeq Size: 1787 bp
• RefSeq ORF: 1023 bp
• Locus ID: 56289
• UniProt ID: Q99MK9
• Cytogenetics: 9 F1
• MW: 39.2 kDa
• Gene Summary: Potential tumor suppressor. Required for death receptor-dependent apoptosis. Mediates activation of Mediates activation of STK3/MST2 and STK4/MST1 during Fas-induced apoptosis by preventing their dephosphorylation. When associated with MOAP1, promotes BAX conformational change and translocation to mitochondrial membranes in response to TNF and TNFSF10 stimulation. Isoform A interacts with CDC20, an activator of the anaphase-promoting complex, APC, resulting in the inhibition of APC activity and mitotic progression. Inhibits proliferation by negatively regulating cell cycle progression at the level of G1/S-phase transition by regulating accumulation of cyclin D1 protein. Isoform C has been shown not to perform these roles, no function has been identified for this isoform. Isoform A disrupts interactions among MDM2, DAXX and USP7, thus contributing to the efficient activation of TP53 by promoting MDM2 self-ubiquitination in cell-cycle checkpoint control in response to DNA damage (By similarity).[UniProtKB/Swiss-Prot Function]