Tex264 (NM_001081654) Mouse Tagged ORF Clone

CAT#: MR229148

Tex264 (myc-DDK-tagged) - Mouse testis expressed gene 264 (Tex264), transcript variant 2

Product Images

Circular map for MR229148

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Tex264
• Synonyms: TEG-264
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR229148 representing NM_001081654
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGGATCTCCTACTACTGGGCCTGATTGGGGCCCTGACGCTGCTGTTGCTGCTGACGCTGCTGGCCT
  TTGCTGGTTATTCAGGACTGCTGACTGGGGTGACAGTGAGCGCTGGATCACCCCCAATCCGCAACATAAC
  TGTGGCCTACAAGTTCCACGTGGGGTCCTATGGTGACACTGGGCACCTTTTCACAGAGAGCTGCAGCATC
  TCTCCCAAGCTCCGTTCCATCGCTGTCTACTATGACAACCCCCATACGGTGCCTCCTGAGAAGTGCCGCT
  GTGCAGTCGGCAGCATCCTGAGTGAGGGGGAGGAGTCGCCTTCACCTGAGCTCATCCACCTCTATCAGAA
  ATTTGGCTTCAAGATATTCTCCTTCCCAGCACCTAGCCATGTGGTCATAGCTACCTTCCCTTACACCACC
  CCCATATCCATCTGGCTGGCTGCCCGCCGAGTCCATCCTGCCTTGGATACCTACATCAAGGAGCGGAAGC
  TGTGTGCTCACCCTCGCCTGGAGATCTACCAGCAAGACAAGATCCATTTCATGTGCCCACTGGCAAGGCA
  AGGAGATTTCTACGTGCCAGAGGTGAAGGAGACAGAGCGGAAATGCCGGGAGCTTGCGGAGGCCACTGAC
  ACCCAGACGGATGGCACAGGAGCTGATACAAGTGATGCAAGTTCTGTGAGCCTGGATGTTCGCCCTGGCA
  GCCGGGAGACTTCAGCCACCACACTTTCTCCTGGGGCAGGCAACCGTGGCTGGGACGACGGTGACAACCG
  CAGCGAGCACAGCTACAGTGAATCGGGTGCCAGTGGCTCGTCCTTTGAGGAGCTGGACCTGGAGGGCGAG
  GGACCCTTGGGAGAACCCCGACTGAACCCTGAAGCCAAGCTTCTGGGGCCCCCTCGGGAGCTCAGCACCC
  CTGAGAGGGGTGAGGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR229148 representing NM_001081654
  Red=Cloning site Green=Tags(s)
  
  MPDLLLLGLIGALTLLLLLTLLAFAGYSGLLTGVTVSAGSPPIRNITVAYKFHVGSYGDTGHLFTESCSI
  SPKLRSIAVYYDNPHTVPPEKCRCAVGSILSEGEESPSPELIHLYQKFGFKIFSFPAPSHVVIATFPYTT
  PISIWLAARRVHPALDTYIKERKLCAHPRLEIYQQDKIHFMCPLARQGDFYVPEVKETERKCRELAEATD
  TQTDGTGADTSDASSVSLDVRPGSRETSATTLSPGAGNRGWDDGDNRSEHSYSESGASGSSFEELDLEGE
  GPLGEPRLNPEAKLLGPPRELSTPERGEE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001081654
• ORF Size: 927 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001081654.1, NM_001081654.2, NP_001075123.1
• RefSeq Size: 1451 bp
• RefSeq ORF: 930 bp
• Locus ID: 21767
• UniProt ID: E9Q137
• Cytogenetics: 9 F1
• MW: 33.6 kDa
• Gene Summary: Major reticulophagy (also called ER-phagy) receptor that acts independently of other candidate reticulophagy receptors to remodel subdomains of the endoplasmic reticulum into autophagosomes upon nutrient stress, which then fuse with lysosomes for endoplasmic reticulum turnover. The ATG8-containing isolation membrane (IM) cradles a tubular segment of TEX264-positive ER near a three-way junction, allowing the formation of a synapse of 2 juxtaposed membranes with trans interaction between the TEX264 and ATG8 proteins. Expansion of the IM would extend the capture of ER, possibly through a 'zipper-like' process involving continued trans TEX264-ATG8 interactions, until poorly understood mechanisms lead to the fission of relevant membranes and, ultimately, autophagosomal membrane closure. Also involved in the repair of covalent DNA-protein cross-links (DPCs) during DNA synthesis: acts by bridging VCP/p97 to covalent DNA-protein cross-links (DPCs) and initiating resolution of DPCs by SPRTN.[UniProtKB/Swiss-Prot Function]