Nefm (NM_008691) Mouse Tagged ORF Clone

CAT#: MR227367

Nefm (Myc-DDK-tagged) - Mouse neurofilament, medium polypeptide (Nefm)

Product Images

Circular map for MR227367

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Nefm
• Synonyms: Nef3; NF-M; NF160; NF165; Nfm
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR227367 representing NM_008691
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCTACACGCTGGACTCGCTGGGCAACCCGTCCGCCTACCGGCGGGTCACCGAGACCCGCTCCAGCT
  TCAGCCGCGTGAGCGGTTCCCCGTCCAGCGGCTTCCGCTCGCAGTCCTGGTCCCGCGGCTCGCCCAGCAC
  CGTGTCCTCCTCCTACAAGCGCAGCGCGCTCGCCCCGCGTCTCGCCTACAGCTCGGCTATGCTCAGCTCG
  GCCGAGAGCAGCCTCGACTTCAGCCAGTCCTCGTCGCTGCTCAACGGCGGCTCCGGCGGCGACTACAAAC
  TGTCCCGCTCTAACGAGAAAGAGCAGCTGCAGGGGCTGAACGACCGCTTCGCCGGCTACATCGAGAAAGT
  GCACTACTTGGAACAACAGAACAAGGAGATCGAAGCAGAGATCCAGGCACTGCGGCAGAAGCAGGCCTCG
  CACGCCCAGCTGGGTGATGCTTACGACCAGGAGATCCGAGAGCTGCGCGCCACCCTCGAGATGGTGAACC
  ACGAGAAGGCTCAAGTGCAGCTGGACTCCGATCACTTGGAGGAAGACATCCACCGGCTCAAGGAGCGCTT
  CGAGGAGGAGGCGCGGCTGCGGGACGACACCGAGGCTGCCATTCGCGCGCTGCGCAAAGACATCGAAGAG
  TCGTCGATGGTTAAGGTGGAGCTGGACAAGAAGGTGCAGTCGCTGCAGGATGAGGTGGCTTTCCTGCGGA
  GCAATCACGAAGAGGAGGTGGCCGACCTGCTGGCTCAGATCCAGGCGTCGCACATCACGGTAGAGCGCAA
  AGATTACCTGAAGACAGACATCTCCACGGCGCTGAAGGAGATCCGCTCCCAGCTCGAGTGTCACTCAGAC
  CAGAACATGCACCAGGCCGAAGAGTGGTTCAAATGCCGCTACGCCAAGCTCACCGAGGCGGCCGAGCAGA
  ACAAGGAGGCCATTCGCTCTGCCAAGGAAGAGATCGCCGAGTACCGGCGCCAGCTGCAGTCCAAGAGCAT
  CGAGCTCGAGTCGGTGCGAGGCACTAAGGAGTCCCTGGAGCGGCAGCTCAGCGACATCGAGGAGCGCCAC
  AACCACGACCTCAGCAGCTACCAGGACACCATCCAGCAGTTGGAAAATGAACTTCGGGGAACCAAGTGGG
  AAATGGCTCGTCATTTGCGAGAATACCAGGATCTCCTTAACGTCAAGATGGCCCTGGACATCGAGATCGC
  CGCGTACAGGAAACTCCTAGAGGGGGAAGAGACCAGATTTAGCACATTTTCAGGAAGCATCACCGGGCCT
  CTGTACACACACCGACAGCCCTCAGTCACAATATCCAGTAAGATTCAGAAGACCAAAGTCGAGGCCCCCA
  AGCTCAAGGTCCAACACAAATTTGTGGAGGAGATCATCGAAGAAACTAAAGTGGAAGATGAGAAGTCAGA
  AATGGAAGAAACCCTCACAGCCATCGCAGAGGAGTTGGCAGCCTCCGCCAAAGAGGAGAAGGAAGAGGCC
  GAAGAAAAGGAGGAGGAACCAGAAGCCGAAAAGTCTCCCGTGAAGTCTCCTGAGGCTAAGGAAGAGGAGG
  AGGAAGGGGAAAAGGAGGAAGAAGAGGAAGGCCAGGAGGAAGAAGAGGAGGAAGATGAAGGTGTCAAGTC
  AGACCAGGCAGAAGAGGGGGGATCTGAGAAGGAAGGCTCCAGTGAAAAAGATGAAGGTGAGCAGGAAGAA
  GAAGAAGGAGAAACCGAGGCAGAAGGTGAAGGAGAGGAAGCAGAAGCTAAGGAGGAAAAGAAAATTGAGG
  GAAAGGTTGAGGAAGTGGCTGTCAAGGAGGAAATCAAGGTCGAGAAGCCTGAGAAAGCCAAATCCCCTAT
  GCCCAAATCACCCGTGGAAGAAGTAAAGCCAAAACCAGAGGCCAAGGCCGGGAAGGGTGAGCACAAGGAG
  GAAGAGAAAGTTGAGGAAGAGAAGAAGGAAGTCACCAAAGAATCACCCAAGGAAGAGAAGGTGGAGAAAA
  AGGAGGAGAAGCCAAAAGATGTTGCAGATAAAAAGAAGGCCGAGTCCCCGGTGAAAGAGAAGGCTGTGGA
  GGAGGTGATCACCATCAGCAAGTCGGTAAAGGTGAGCCTAGAGAAAGACACCAAAGAGGAGAAGCTGCAG
  CCGCAGGAGAAGGTGAAGGAGAAGGCAGAGGAGGAGGGGGGCAGTGAGGAGGAAGGGAGTGACCGTAGCC
  CGCAGGAGTCCAAGAAGGAAGACATAGCTATCAATGGGGAGGTGGAAGGAAAAGAGGAGGAGGAGCAGGA
  AACTCAGGAGAAGGGCAGTGGGCGGGAGGAGGAGAAAGGGGTGGTCACTAATGGCTTAGATGTGAGCCCT
  GCAGAGGAGAAGAAAGGAGAGGATAGCAGTGATGATAAAGTGGTGGTCACCAAGAAGGTAGAAAAGATCA
  CCAGCGAGGGGGGCGATGGTGCTACCAAATACATCACCAAATCTGTAACCGTCACTCAAAAGGTTGAAGA
  GCATGAGGAGACCTTTGAGGAGAAGCTGGTCTCAACTAAAAAGGTAGAAAAGGTCACTTCACACGCCATA
  GTCAAGGAAGTCACCCAGGGTGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR227367 representing NM_008691
  Red=Cloning site Green=Tags(s)
  
  MSYTLDSLGNPSAYRRVTETRSSFSRVSGSPSSGFRSQSWSRGSPSTVSSSYKRSALAPRLAYSSAMLSS
  AESSLDFSQSSSLLNGGSGGDYKLSRSNEKEQLQGLNDRFAGYIEKVHYLEQQNKEIEAEIQALRQKQAS
  HAQLGDAYDQEIRELRATLEMVNHEKAQVQLDSDHLEEDIHRLKERFEEEARLRDDTEAAIRALRKDIEE
  SSMVKVELDKKVQSLQDEVAFLRSNHEEEVADLLAQIQASHITVERKDYLKTDISTALKEIRSQLECHSD
  QNMHQAEEWFKCRYAKLTEAAEQNKEAIRSAKEEIAEYRRQLQSKSIELESVRGTKESLERQLSDIEERH
  NHDLSSYQDTIQQLENELRGTKWEMARHLREYQDLLNVKMALDIEIAAYRKLLEGEETRFSTFSGSITGP
  LYTHRQPSVTISSKIQKTKVEAPKLKVQHKFVEEIIEETKVEDEKSEMEETLTAIAEELAASAKEEKEEA
  EEKEEEPEAEKSPVKSPEAKEEEEEGEKEEEEEGQEEEEEEDEGVKSDQAEEGGSEKEGSSEKDEGEQEE
  EEGETEAEGEGEEAEAKEEKKIEGKVEEVAVKEEIKVEKPEKAKSPMPKSPVEEVKPKPEAKAGKGEHKE
  EEKVEEEKKEVTKESPKEEKVEKKEEKPKDVADKKKAESPVKEKAVEEVITISKSVKVSLEKDTKEEKLQ
  PQEKVKEKAEEEGGSEEEGSDRSPQESKKEDIAINGEVEGKEEEEQETQEKGSGREEEKGVVTNGLDVSP
  AEEKKGEDSSDDKVVVTKKVEKITSEGGDGATKYITKSVTVTQKVEEHEETFEEKLVSTKKVEKVTSHAI
  VKEVTQGD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008691
• ORF Size: 2544 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008691.2, NP_032717.2
• RefSeq Size: 3244 bp
• RefSeq ORF: 2547 bp
• Locus ID: 18040
• UniProt ID: P08553
• Cytogenetics: 14 D1
• MW: 96.4 kDa
• Gene Summary: Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber.[UniProtKB/Swiss-Prot Function]