Mst1r (NM_009074) Mouse Tagged ORF Clone

CAT#: MR227344

Mst1r (Myc-DDK-tagged) - Mouse macrophage stimulating 1 receptor (c-met-related tyrosine kinase) (Mst1r)

Product Images

Circular map for MR227344

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Mst1r
• Synonyms: CD136; CDw136; Fv; Fv-; Fv-2; Fv2; PTK; PTK8; Ron; ST; STK
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR227344 representing NM_009074
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  CTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGCTGCCTCTGCCGCTGCTTCAATCCTCTCTTCTGCTAATGCTTCTTTTGCGGCTGTCGGCGGCGT
  CCACCAACCTGAACTGGCAGTGCCCACGAATACCCTACGCAGCCTCCCGAGACTTCAGTGTCAAGTACGT
  GGTCCCCAGCTTCTCCGCGGGGGGCCGGGTACAGGCCACCGCAGCCTACGAGGACAGTACAAATAGTGCG
  GTGTTTGTGGCCACACGCAATCACCTGCACGTGCTTGGGCCTGACCTGCAGTTCATAGAGAACCTGACCA
  CTGGCCCTATCGGGAACCCTGGCTGCCAGACTTGTGCGAGCTGTGGTCCAGGCCCTCATGGACCACCAAA
  GGACACAGACACACTGGTGCTAGTGATGGAGCCAGGTTTGCCAGCCCTGGTCAGCTGTGGCTCAACCCTA
  CAGGGCCGCTGCTTCCTGCATGAGCTGGAGCCTCGGGGGAAAGCCCTGCACTTAGCAGCTCCAGCCTGCC
  TATTCTCAGCAAACAATAACAAGCCTGAGGCCTGCACGGACTGTGTGGCTAGCCCCCTGGGCACTCGTGT
  GACTGTGGTGGAGCAGGGGCATGCTTCCTACTTCTATGTGGCATCTTCGCTAGACCCAGAGTTGGCCGCT
  AGCTTTAGCCCCCGCTCGGTGTCCATCCGTCGTCTAAAGTCTGATACTTCTGGATTCCAACCAGGTTTTC
  CGTCGCTGTCGGTGCTGCCCAAATATTTGGCCTCCTACCTCATCAAATATGTGTACAGCTTCCACTCGGG
  GGATTTTGTCTACTTTCTGACTGTCCAGCCCATCAGTGTCACAAGCCCTCCCAGTGCCTTGCATACACGT
  CTGGTCCGGCTCAATGCTGTAGAGCCAGAGATTGGTGACTACCGGGAGCTGGTCTTGGACTGTCATTTTG
  CACCTAAACGCCGGCGCCGTGGAGCCCCGGAGGGCACACAGCCCTACCCAGTGCTTCAGGCAGCCCACTC
  TGCTCCAGTGGATGCCAAACTGGCTGTGGAACTGAGCATTTCAGAGGGCCAGGAAGTGCTTTTTGGGGTC
  TTTGTGACCGTCAAGGATGGTGGCTCTGGCATGGGTCCCAACTCTGTTGTATGTGCCTTCCCCATTTACC
  ACCTGAACATCCTGATTGAAGAGGGTGTCGAATATTGCTGTCACTCTTCAAATTCTTCTTCCCTGTTGTC
  GAGAGGCCTTGACTTCTTCCAGACGCCCAGTTTTTGTCCTAATCCGCCTGGTGGAGAGGCCTCCGGCCCC
  AGCTCCCGTTGCCACTACTTCCCTTTGATGGTCCACGCTAGCTTCACCCGTGTGGACCTCTTCAATGGAC
  TGTTAGGATCAGTGAAGGTCACCGCACTGCATGTGACACGTCTTGGCAATGTTACAGTGGCCCACATGGG
  CACTGTGGATGGGCGTGTCCTACAGGTGGAGATAGCCAGGTCACTCAACTACCTGCTGTATGTGTCCAAC
  TTCTCCCTGGGCAGCAGTGGACAGCCTGTTCATCGGGATGTCAGCCGCCTCGGGAATGACCTACTCTTTG
  CCTCTGGGGACCAGGTCTTCAAGGTGCCCATCCAGGGCCCTGGCTGTCGTCATTTTCTCACCTGTTGGCG
  TTGCCTGAGAGCACAGCGCTTCATGGGATGTGGCTGGTGTGGGGACCGGTGTGACCGGCAGAAGGAGTGT
  CCTGGCTCCTGGCAACAGGACCACTGTCCGCCTGAGATCAGTGAGTTCTATCCTCACAGCGGGCCTCTAA
  GGGGCACTACGAGGCTCACCCTTTGTGGCTCCAACTTCTACCTGCGACCTGATGATGTCGTACCTGAGGG
  AACACACCAGATCACCGTGGGCCAAAGTCCCTGCCGACTGCTGCCTAAGGACTCTTCAAGCCCTAGGCCA
  GGGTCCCTCAAGGAATTCATACAGGAACTTGAATGTGAGCTGGAGCCCCTGGTCACCCAGGCAGTGGGGA
  CTACAAACATCAGCCTTGTCATCACCAACATGCCAGCAGGCAAGCACTTCCGAGTGGAAGGCATCTCTGT
  ACAGGAAGGCTTCTCTTTCGTGGAGCCAGTGCTGACATCAATAAAACCTGACTTTGGCCCGCGGGCTGGG
  GGTACTTATCTCACCCTTGAAGGCCAGAGCCTGTCTGTCGGCACCAGCCGGGCTGTGCTGGTCAATGGAA
  CCCAGTGCCGGCTGGAACAGGTCAATGAGGAGCAGATCTTATGTGTCACGCCTCCTGGAGCTGGCACGGC
  CAGGGTCCCCCTTCATCTGCAGATAGGGGGTGCTGAGGTGCCTGGCTCCTGGACCTTTCACTACAAGGAA
  GACCCTATTGTGTTGGACATCAGTCCCAAGTGTGGCTACAGTGGCTCCCACATCATGATCCATGGCCAGC
  ATCTGACTTCAGCATGGCACTTCACGCTATCATTCCATGATGGACAAAGTACAGTGGAGAGCAGGTGTGC
  GGGGCAGTTTGTGGAACAACAGCAGCGTCGATGTCGCCTGCCTGAATATGTGGTCCGAAACCCTCAGGGG
  TGGGCAACAGGGAATCTGAGCGTCTGGGGTGATGGAGCAGCTGGCTTCACGCTGCCTGGTTTTCGCTTCC
  TGCCCCCACCCAGTCCACTCAGAGCTGGCCTGGTTGAGTTGAAACCTGAAGAACATTCAGTTAAAGTTGA
  GTATGTCGGGCTGGGCGCTGTGGCAGACTGTGTGACTGTGAACATGACCGTGGGTGGTGAGGTCTGCCAA
  CATGAGCTCCGGGGGGATGTGGTGATCTGCCCCCTGCCCCCTTCCCTGCAACTTGGCAAGGATGGTGTCC
  CATTGCAGGTCTGTGTAGACGGTGGGTGTCACATCCTGAGCCAAGTGGTTCGCTCAAGCCCAGGCAGGGC
  CTCACAGAGGATACTCCTTATTGCTCTTCTGGTCTTGATCCTGCTTGTGGCTGTGCTGGCCGTTGCCCTG
  ATCTTTAACTCCCGAAGACGGAAAAAGCAGCTAGGTGCTCACTCCCTCTCCCCAACAACACTCTCTGACA
  TCAACGATACAGCTTCCGGGGCTCCGAACCATGAAGAATCGTCAGAGAGTAGGGATGGGACAAGTGTCCC
  ACTGCTGCGGACAGAGTCTATCCGGCTCCAGGATCTGGACAGGATGCTCCTAGCTGAGGTCAAGGATGTA
  CTGATTCCCCATGAACAAGTGGTCATCCATACTGACCAAGTCATTGGCAAAGGCCACTTTGGTGTTGTCT
  ACCACGGAGAATATACAGACGGAGCACAGAATCAGACCCACTGTGCCATCAAGTCTCTGAGTCGCATTAC
  AGAGGTGCAGGAGGTGGAGGCTTTCCTGCGGGAGGGGCTGCTCATGCGTGGCCTACATCACCCAAACATC
  CTGGCTCTCATCGGTATCATGCTGCCCCCGGAGGGGCTTCCCCGGGTGCTGTTGCCCTATATGCGCCACG
  GAGACCTGCTTCATTTCATTCGCTCCCCTCAGAGGAACCCCACTGTGAAGGATCTTGTCAGCTTTGGCCT
  GCAGGTAGCCTGTGGTATGGAGTACCTGGCAGAGCAGAAGTTCGTGCACAGAGACCTGGCTGCTAGGAAC
  TGCATGCTGGACGAGTCATTCACAGTCAAGGTGGCTGACTTTGGTCTGGCACGGGGCGTCCTAGACAAGG
  AATACTACAGTGTTCGCCAGCATCGCCATGCTCGCCTGCCAGTCAAATGGATGGCACTGGAGAGCCTGCA
  GACCTACAGGTTCACCACCAAGTCCGATGTGTGGTCATTCGGGGTGCTGCTCTGGGAGCTACTAACACGG
  GGTGCTCCACCCTACCCCCATATCGATCCCTTCGACCTCTCTCACTTCCTGGCTCAGGGCCGTCGCCTGC
  CTCAGCCTGAGTACTGTCCTGATTCACTGTATCACGTGATGCTTCGATGCTGGGAGGCTGACCCAGCGGC
  ACGACCCACCTTCAGAGCCCTAGTGCTGGAAGTAAAGCAGGTAGTGGCCTCACTGCTTGGGGACCACTAT
  GTGCAGCTGACAGCAGCTTATGTGAACGTAGGCCCCAGAGCGGTGGATGATGGGAGTGTGCCTCCGGAGC
  AGGTACAGCCCTCGCCTCAGCATTGCAGGAGCACGTCAAAGCCCCGGCCTCTCTCAGAGCCACCCCTGCC
  CACT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009074
• ORF Size: 4134 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009074.2, NP_033100.2
• RefSeq Size: 4706 bp
• RefSeq ORF: 4137 bp
• Locus ID: 19882
• UniProt ID: Q62190
• Cytogenetics: 9 F1
• MW: 151 kDa
• Gene Summary: This gene encodes a precursor protein that is proteolytically cleaved to yield an alpha chain and a beta chain which form a membrane-spanning heterodimer. The encoded protein belongs to a family of cell-surface receptor tyrosine kinases involved in signaling from the cell surface to the intracellular environment. The binding of the encoded protein to its ligand, macrophage-stimulating protein, mediates several biological activities including wound healing, tumor immunity, macrophage activation and hematopoiesis as well as cell growth, motility, survival and adhesion. The protein encoded by this gene also functions in early development and the macrophage-mediated inflammatory response. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2013]