Rb1 (NM_009029) Mouse Tagged ORF Clone

CAT#: MR227287

Rb1 (Myc-DDK-tagged) - Mouse retinoblastoma 1 (Rb1)

Product Images

Circular map for MR227287

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Rb1
• Synonyms: pRb; Rb; Rb-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR227287 representing NM_009029
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGCCCAAAGCCCCGCGCAGAGCCGCGGCCGCCGAGCCCCCGCCACCGCCGCCGCCGCCGCCTCGGG
  AGGACGACCCCGCGCAGGACAGCGGCCCCGAAGAGCTGCCCCTGGCCAGGCTTGAGTTTGAAGAAATTGA
  AGAACCCGAATTTATTGCATTATGTCAAAAGTTAAAGGTACCCGATCATGTCAGAGAAAGAGCTTGGCTA
  ACTTGGGAGAAAGTTTCATCCGTGGATGGAATCCTGGAAGGATATATTCAAAAGAAGAAGGAACTCTGGG
  GCATCTGCATCTTTATCGCAGCAGTTGATCTAGATGAGATGCCATTCACTTTTACTGAGCTACAGAAAAG
  CATAGAAACCAGTGTCTATAAATTCTTTGACTTATTAAAAGAAATCGATACCAGTACCAAGGTTGATAAT
  GCTATGTCAAGACTATTGAAGAAGTATAATGTGTTATGTGCACTCTACAGCAAATTAGAACGGACGTGTG
  AACTTATATATTTGACACAACCCAGCAGTGCGTTATCTACTGAAATAAATTCTATGTTGGTGCTAAAAAT
  TTCTTGGATCACTTTTTTACTAGCTAAAGGAGAAGTATTACAGATGGAAGATGACCTGGTAATCTCATTT
  CAGCTAATGTTGTGTGTAGTTGACTATTTTATTAAGTTCTCACCTCCTGCACTACTCAGAGAGCCATACA
  AAACAGCTGCAATCCCCATTAATGGTTCACCTCGAACACCCAGAAGAGGTCAGAACAGGAGCGCTCGGAT
  AGCAAAACAACTAGAAAATGATACGAGGATTATCGAGGTTCTCTGTAAAGAACACGAGTGTAATATAGAT
  GAGGTGAAAAATGTTTATTTCAAAAATTTTATCCCTTTTATAAATTCACTTGGAATTGTATCATCTAATG
  GACTTCCAGAGGTTGAAAGTCTTTCTAAACGCTATGAAGAAGTTTATCTTAAAAACAAAGATTTAGATGC
  AAGACTGTTTTTGGATCATGATAAAACACTTCAGACTGATCCTATAGACAGTTTTGAAACAGAGAGAACG
  CCACGAAAAAACAACCCTGATGAAGAGGCAAACGTGGTTACTCCACACACTCCAGTTAGGACTGTTATGA
  ATACTATTCAACAATTAATGGTGATTTTAAATTCTGCAAGTGATCAGCCATCAGAAAATCTGATTTCCTA
  CTTCAATAATTGCACAGTGAATCCAAAAGAAAATATCCTAAAGAGAGTAAAGGATGTTGGGCACATCTTT
  AAAGAGAAGTTTGCTAACGCTGTGGGCCAGGGCTGTGTTGACATCGGAGTACAGCGATATAAACTTGGAG
  TCCGATTGTATTACCGTGTGATGGAATCCATGCTTAAATCAGAAGAAGAACGTTTGTCCATTCAGAATTT
  TAGCAAACTCCTAAATGACAACATCTTTCATATGTCTTTACTGGCCTGTGCTCTTGAAGTTGTAATGGCT
  ACGTATAGCAGAAGTACATTGCAGCATCTTGATTCTGGAACAGATTTGTCCTTCCCGTGGATTCTGAACG
  TACTTAATTTAAAAGCCTTTGATTTTTACAAAGTGATTGAAAGTTTTATCAAAGTGGAAGCCAACTTGAC
  AAGAGAAATGATAAAACATTTAGAAAGATGTGAGCATCGAATCATGGAATCCCTTGCATGGCTTTCAGAT
  TCACCTTTATTTGATCTCATTAAGCAGTCCAAGGATGGAGAAGGACCTGATAACCTTGAACCTGCTTGTC
  CTCTCAGCCTGCCTCTCCAGGGTAACCATACTGCAGCAGATATGTATCTTTCTCCTCTAAGATCTCCAAA
  GAAAAGAACTTCCACTACACGTGTAAATTCTGCTGCAAATACAGAGACACAAGCAGCCTCAGCCTTCCAT
  ACTCAGAAGCCATTGAAATCTACCTCCCTTGCCCTGTTTTACAAAAAAGTGTACCGTCTAGCATATCTCC
  GACTAAATACACTCTGTGCACGCCTTCTGTCTGACCACCCAGAGCTAGAGCACATCATCTGGACTCTGTT
  TCAGCATACATTGCAAAATGAGTATGAGCTCATGAGAGACCGACATTTGGACCAGATTATGATGTGCTCT
  ATGTATGGCATCTGCAAGGTGAAGAACATCGACCTCAAGTTCAAAATCATCGTCACTGCCTACAAGGATC
  TTCCTCACGCTGCCCAGGAGACCTTTAAACGTGTTTTGATCAGAGAAGAGGAGTTTGATTCCATTATAGT
  ATTCTATAACTCCGTTTTCATGCAGAGACTAAAAACAAATATTTTACAGTATGCCTCCACCAGGCCTCCT
  ACCTTGTCACCAATACCTCACATTCCTCGAAGCCCTTACAAGTTTTCTAGTTCACCCTTACGGATTCCTG
  GAGGTAACATCTATATATCACCCCTAAAGAGTCCTTATAAAATTTCAGAAGGTCTGCCAACACCCACAAA
  AATGACTCCGAGATCAAGAATCTTGGTCTCAATTGGTGAATCATTTGGGACATCTGAAAAGTTCCAGAAA
  ATAAACCAGATGGTGTGTAATAGTGACAGAGTGCTCAAAAGAAGTGCTGAAGGCGGCAACCCCCCCAAAC
  CACTGAAAAAGCTGCGCTTTGACATCGAGGGAGCCGATGAAGCAGATGGGAGTAAACATCTCCCAGCGGA
  GTCCAAATTCCAACAGAAACTGGCAGAAATGACTTCCACTCGAACACGAATGCAAAAGCAGAGAATGAAT
  GAGAGCAAGGATGTCTCAAACAAGGAGGAAAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR227287 representing NM_009029
  Red=Cloning site Green=Tags(s)
  
  MPPKAPRRAAAAEPPPPPPPPPREDDPAQDSGPEELPLARLEFEEIEEPEFIALCQKLKVPDHVRERAWL
  TWEKVSSVDGILEGYIQKKKELWGICIFIAAVDLDEMPFTFTELQKSIETSVYKFFDLLKEIDTSTKVDN
  AMSRLLKKYNVLCALYSKLERTCELIYLTQPSSALSTEINSMLVLKISWITFLLAKGEVLQMEDDLVISF
  QLMLCVVDYFIKFSPPALLREPYKTAAIPINGSPRTPRRGQNRSARIAKQLENDTRIIEVLCKEHECNID
  EVKNVYFKNFIPFINSLGIVSSNGLPEVESLSKRYEEVYLKNKDLDARLFLDHDKTLQTDPIDSFETERT
  PRKNNPDEEANVVTPHTPVRTVMNTIQQLMVILNSASDQPSENLISYFNNCTVNPKENILKRVKDVGHIF
  KEKFANAVGQGCVDIGVQRYKLGVRLYYRVMESMLKSEEERLSIQNFSKLLNDNIFHMSLLACALEVVMA
  TYSRSTLQHLDSGTDLSFPWILNVLNLKAFDFYKVIESFIKVEANLTREMIKHLERCEHRIMESLAWLSD
  SPLFDLIKQSKDGEGPDNLEPACPLSLPLQGNHTAADMYLSPLRSPKKRTSTTRVNSAANTETQAASAFH
  TQKPLKSTSLALFYKKVYRLAYLRLNTLCARLLSDHPELEHIIWTLFQHTLQNEYELMRDRHLDQIMMCS
  MYGICKVKNIDLKFKIIVTAYKDLPHAAQETFKRVLIREEEFDSIIVFYNSVFMQRLKTNILQYASTRPP
  TLSPIPHIPRSPYKFSSSPLRIPGGNIYISPLKSPYKISEGLPTPTKMTPRSRILVSIGESFGTSEKFQK
  INQMVCNSDRVLKRSAEGGNPPKPLKKLRFDIEGADEADGSKHLPAESKFQQKLAEMTSTRTRMQKQRMN
  ESKDVSNKEEK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009029
• ORF Size: 2763 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009029.3
• RefSeq Size: 4642 bp
• RefSeq ORF: 2766 bp
• Locus ID: 19645
• UniProt ID: P13405
• Cytogenetics: 14 D3
• MW: 105.8 kDa
• Gene Summary: Key regulator of entry into cell division that acts as a tumor suppressor. Promotes G0-G1 transition when phosphorylated by CDK3/cyclin-C. Acts as a transcription repressor of E2F1 target genes. The underphosphorylated, active form of RB1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV39H1, KMT5B and KMT5C, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Inhibits the intrinsic kinase activity of TAF1. Mediates transcriptional repression by SMARCA4/BRG1 by recruiting a histone deacetylase (HDAC) complex to the c-FOS promoter. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex (By similarity).[UniProtKB/Swiss-Prot Function]