Tjp1 (NM_001163574) Mouse Tagged ORF Clone

CAT#: MR226867

Tjp1 (Myc-DDK-tagged) - Mouse tight junction protein 1 (Tjp1), transcript variant 2

Product Images

Circular map for MR226867

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Tjp1
• Synonyms: ZO1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR226867 representing NM_001163574
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCGCCAGGGCCGCGGCCGCTAAGAGCACAGCAATGGAGGAAACAGCTATATGGGAACAGCACACAG
  TGACGCTTCACAGGGCTCCTGGGTTTGGATTTGGAATTGCAATCTCTGGTGGAAGAGATAATCCTCATTT
  TCAGAGTGGGGAAACCTCCATAGTGATTTCTGATGTGTTAAAAGGAGGGCCAGCTGAAGGACAGCTACAG
  GAAAATGACCGAGTTGCAATGGTTAACGGAGTTTCAATGGATAACGTTGAACATGCTTTTGCTGTTCAGC
  AGCTAAGGAAGAGTGGGAAAAACGCAAAAATTACTATCCGAAGGAAGAAGAAAGTTCAGATCCCTGTAAG
  TCACCCAGATCCTGAGCCGGTGTCTGATAATGAAGACGATAGTTATGACGAAGAAGTGCATGACCCAAGA
  GCTGGCCGCGGTGCTTTAGCGAACAGAAGGAGCGAGAAGAGCTGGGCAAGGGATAGGAGTGCAAGCAGGG
  AGAGGAGCCTGTCCCCTCGCTCGGACAGGCGGTCCGTGGCCTCCAGTCAGCCCGCAAAGCCCACCAAGGT
  CACACTGGTGAAGTCTCGGAAAAATGAAGAATATGGTCTTCGATTGGCCAGCCATATATTTGTAAAGGAA
  ATTTCACAAGATAGTTTGGCAGCAAGAGATGGTAACATTCAAGAAGGGGATGTTGTCTTGAAGATAAATG
  GTACTGTGACAGAAAATATGTCATTGACAGATGCAAAAACACTGATAGAAAGGTCTAAAGGCAAGTTAAA
  AATGGTAGTGCAAAGAGATGAGCGGGCTACCTTACTGAACGTCCCTGACCTTTCGGATAGTATCCATTCT
  GCTAATGCCTCGGAAAGAGATGACATTTCAGAAATTCAGTCACTAGCATCAGACCATTCAGGTCGCTCGC
  ATGACAGGCCACCCCGCCGCAGCCAGTCACGATCTCCTGACCAACGTTCAGAGCCCTCCGATCATTCCAC
  GCAGTCTCCACAGCAGCCCAGCAATGGCAGTCTCCGGAGCAGAGAGGAAGAGCGAATGTCTAAACCTGGG
  GCCATCTCAACTCCTGTAAAACATGTAGACGATCATCCACCCAAAGCAGTGGAAGAAGTTACAGTTGAGA
  AAAATGAGAAGCAGACGCCCACTCTTCCAGAACCGAAACCTGTGTATGCTCAAGTTGGACAACCAGATGT
  GGATTTACCCGTCAGCCCTTCTGATGGTGCTCTGCCTAATTCAGCTCATGAAGACGGGATACTTAGGCCC
  AGCATGAAACTGGTAAAATTCAGAAAAGGAGATAGTGTGGGTTTGCGACTAGCTGGTGGAAATGATGTCG
  GAATATTTGTAGCTGGCGTTCTAGAAGATAGCCCTGCAGCCAAAGAAGGCTTAGAGGAAGGTGATCAAAT
  TCTCAGGGTGAACAATGTAGATTTCACAAATATCATAAGGGAAGAGGCCGTCCTTTTCCTCCTTGACCTC
  CCTAAAGGTGAAGAAGTGACCATACTGGCTCAGAAGAAGAAGGACGTTTATCGCCGCATTGTAGAATCAG
  ATGTAGGAGATTCATTCTATATTAGAACGCATTTTGAATATGAAAAAGAATCTCCTTACGGACTTAGTTT
  TAACAAAGGAGAGGTGTTCCGGGTCGTGGATACTTTATACAATGGAAAGCTGGGCTCTTGGCTTGCCATT
  CGAATTGGCAAAAATCATAAGGAGGTAGAACGAGGCATCATCCCTAATAAGAACAGAGCTGAACAGTTAG
  CCAGTGTACAGTACACACTTCCAAAGACAGCGGGTGGTGATCGGGCAGACTTCTGGAGGTTTCGAGGTCT
  TCGCAGCTCCAAGAGAAATCTTCGAAAAAGCAGAGAGGACTTGTCAGCTCAGCCAGTTCAAACTAAGTTC
  CCAGCTTATGAAAGGGTTGTTCTTCGAGAAGCTGGATTCCTAAGACCTGTAACCATCTTTGGACCAATAG
  CTGATGTTGCCAGAGAAAAGTTGGCAAGAGAGGAGCCAGATATCTATCAGATTGCAAAAAGTGAACCACG
  AGATGCTGGGACTGACCATCGTAGCTCTGGCATCATTCGCCTTCATACAATAAAGCAAATCATAGATCAG
  GATAAACATGCTTTATTAGATGTAACGCCAAATGCGGTTGATCGTCTTAATTATGCGCAGTGGTATCCAA
  TTGTTGTGTTCCTTAACCCTGACTCTAAGCAAGGTGTAAAAACAATGAGGATGAGGTTGTGTCCGGAGTC
  TCGGAAAAGCGCCAGGAAGCTATATGAACGCTCTCATAAGCTTCGTAAGAACAATCACCATCTCTTCACA
  ACTACAATTAACTTAAACTCAATGAATGATGGTTGGTACGGTGCCCTGAAAGAAGCGATTCAGCAGCAAC
  AGAACCAGCTGGTGTGGGTCTCTGAGGGGAAGGCGGATGGTGCTACAAGTGATGACCTTGATTTGCATGA
  CGATCGTCTGTCCTACCTGTCAGCCCCAGGTAGTGAGTACTCAATGTATAGCACGGACAGTAGACACACT
  TCTGACTATGAAGACACAGATACAGAAGGCGGGGCCTACACTGATCAAGAACTAGATGAAACTCTTAATG
  ATGAGGTGGGGACTCCCCCGGAGTCTGCCATTACACGGTCCTCTGAGCCTGTAAGAGAGGATTCCTCTGG
  AATGCATCATGAAAACCAGACATACCCTCCTTACTCACCACAAGCGCAGCCACAAGCTATTCATAGAATA
  GACTCCCCTGGACTTAAGCCAGCCTCTCAACAGGTGTACAGGAAGGAGCCGTATTCTGAAGAAATGATGA
  GACAAAACCATATTTTAAAACAACCAGCTCTTGGTCACCCAGGGCAGAGGCCAGATAAAGAGCCAAATCT
  AGCCTATGAACCCCAACTTCCATATATAGAAAAACAAGCCAGCAGAGACCTTGAGCAGCCGTCATACAGG
  TATGAGGTCTCAAGCTACACAGACCAGTTTTCTCGGAACTATGACCATCGCCTACGGTTTGAAGATCGAA
  TCCCTACCTATGAAGACCAGTGGTCATATTATGATGACAAACAGCCCTACCAACCTCGGCCTTTTGAGAA
  TCAGCATCCCCGAGACCTGGACTCCAGACAACATCCCGAAGAGGCTTCAGAACGAGGTTATTTCCAGCGT
  TTTGAAGAGCCAGCCCCTCTGTCGTACGACAGTAGAACACGCTATGAGCAGCTGCCTCGAACCTCTACTC
  TACGACATGAAGAGCAGCCAGCCCCTGCATATGAGGTGCACAACAGGTACAGGCCAGAGGCACAGCCCTA
  TTCTTCAACAGGCCCTAAGTCATCTGAGCCCAAGCAGTACTTTGACCAGTACCCGCGAAGTTATGAGCAA
  GTTCCACCACCAGGCTTTACCTCCAAAACAGGCCATTACGAGCCTCTCCATGGTGCTGCAGTTGTCCCTC
  CTCTGATACCTTCCTCTCAACAAAAGCCAGAAGTCCTGCCCTCGGCTACCAAACCACAGCCTCCACCCCC
  AACCCTAACTGAGGAGGAGGAGGATCCAGCAATGAAACCACAGTCTGTGCTCACCAGAGTCAAAATGTTT
  GAAAACAAAAGATCTGCGTCTTTGGAGAACAAGAAAGATGTGAATGACACTGCCAGCTTTAAGCCTCCGG
  AAGTAGCATCTAAACCTCCAGGTGCTTCTCTTGCTGGCCCTAAACCTGTCCCTCAGAGTCAGTTTAGTGA
  GCACGACAAAACGCTCTACAGGCTCCCAGAGCCTCAGAAACCTCAAGTGAAGCCACCCGAAGATATTGTT
  CGATCAAATCATTACGACCCTGAAGAGGATGAAGAATATTACCGGAAACAGCTCTCCTACTTTGACCGAA
  GAAGTTTTGAGAGCAAGCCTTCTGCACATCTTCCTGCTGGCCATCACTCAGAGCCTGCTAAGCCAGTCCA
  TTCTCAGAGCCAGCCCAACTTCTCTAGTTACTCTTCAAAGGGAAAACCCGAAACTGATGCTGTGGATAGA
  TCATTCAGTGAGAAACGTTATGATCCAGCCCAGGCCACGCCTCCTCCTCCTCCGTTGCCCTCACAGTACA
  GCCAGCCAGCTCCACCTCTGTCCAGCTCTTCTCTCCACATACATTCCAAGGGCGCCCAGGGTGAAGGCAA
  CTCAGTATCATTGGATTTTCAGAACTCATATATGTCCAAACCAGACCCACCCCCATCTCAGAGTAAACCA
  GCAACTTTCAGACCACCAACTCGAGAAGACCCCCCTCAGACTTTCTATCCGCAGAAAAGTTTCCCAGACA
  AAGCTCCAGTTAACGGAGCTGAGCAGACTCAGAAAACCATCACTCCGGTGTACAATCGATTCACACCAAA
  GCCGTACACAAGTTCTGCCCGGCCATTTGAACGCAAATTTGAAAGTCCGAAGTTCAACCATAATCTTCTG
  CCAAGTGAAACTGTACATAAACCTGAATTGTCTTCAAAAACTCCCACTTCCCCAAAAACTCTTATGAAAG
  CTCATAGTTCAACACAGCCTCCAGAGTTTGACAGTGGAGTTGAAACTTTCTCTGTTCACACAGATAAGCC
  TAAATATCAAATGAATAATATCAGCACCATGCCTAAAGCTGTCCCTGTGAGTCCTTCAGCTGTGGAAGAA
  GATGAAGATGAGGATGGTCATACTGTAGTGGCTACAGCTCGTGGCATTTTTAACAGCAATGGTGGTGTGT
  TGAGTTCCATAGAAACCGGTGTTAGTATAATTATCCCACAAGGAGCCATTCCTGAAGGAATTGAGCAAGA
  AATCTATTTCAAAGTCTGCAGAGACAATAGCATTCTCCCACCTTTAGATAAAGAGAAAGGTGAAACTCTG
  CTGAGCCCCCTAGTGATGTGTGGGCCCCATGGCCTCAAGTTCCTGAAGCCCGTGGAGCTACGCTTGCCAC
  ACTGTGCGTCCATGACTCCTGACGGTTGGTCTTTTGCTCTAAAATCATCCGACTCCTCGTCGGGTGACCC
  TAAAACCTGGCAAAACAAGTGTCTTCCTGGAGATCCGAATTACCTTGTTGGAGCCAACTGTGTTTCTGTC
  CTGATTGACCACTTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR226867 representing NM_001163574
  Red=Cloning site Green=Tags(s)
  
  MSARAAAAKSTAMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQ
  ENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSHPDPEPVSDNEDDSYDEEVHDPR
  AGRGALANRRSEKSWARDRSASRERSLSPRSDRRSVASSQPAKPTKVTLVKSRKNEEYGLRLASHIFVKE
  ISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHS
  ANASERDDISEIQSLASDHSGRSHDRPPRRSQSRSPDQRSEPSDHSTQSPQQPSNGSLRSREEERMSKPG
  AISTPVKHVDDHPPKAVEEVTVEKNEKQTPTLPEPKPVYAQVGQPDVDLPVSPSDGALPNSAHEDGILRP
  SMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDL
  PKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAI
  RIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKF
  PAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDHRSSGIIRLHTIKQIIDQ
  DKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFT
  TTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHT
  SDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPPYSPQAQPQAIHRI
  DSPGLKPASQQVYRKEPYSEEMMRQNHILKQPALGHPGQRPDKEPNLAYEPQLPYIEKQASRDLEQPSYR
  YEVSSYTDQFSRNYDHRLRFEDRIPTYEDQWSYYDDKQPYQPRPFENQHPRDLDSRQHPEEASERGYFQR
  FEEPAPLSYDSRTRYEQLPRTSTLRHEEQPAPAYEVHNRYRPEAQPYSSTGPKSSEPKQYFDQYPRSYEQ
  VPPPGFTSKTGHYEPLHGAAVVPPLIPSSQQKPEVLPSATKPQPPPPTLTEEEEDPAMKPQSVLTRVKMF
  ENKRSASLENKKDVNDTASFKPPEVASKPPGASLAGPKPVPQSQFSEHDKTLYRLPEPQKPQVKPPEDIV
  RSNHYDPEEDEEYYRKQLSYFDRRSFESKPSAHLPAGHHSEPAKPVHSQSQPNFSSYSSKGKPETDAVDR
  SFSEKRYDPAQATPPPPPLPSQYSQPAPPLSSSSLHIHSKGAQGEGNSVSLDFQNSYMSKPDPPPSQSKP
  ATFRPPTREDPPQTFYPQKSFPDKAPVNGAEQTQKTITPVYNRFTPKPYTSSARPFERKFESPKFNHNLL
  PSETVHKPELSSKTPTSPKTLMKAHSSTQPPEFDSGVETFSVHTDKPKYQMNNISTMPKAVPVSPSAVEE
  DEDEDGHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPEGIEQEIYFKVCRDNSILPPLDKEKGETL
  LSPLVMCGPHGLKFLKPVELRLPHCASMTPDGWSFALKSSDSSSGDPKTWQNKCLPGDPNYLVGANCVSV
  LIDHF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001163574
• ORF Size: 5055 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001163574.1, NP_001157046.1
• RefSeq Size: 6891 bp
• RefSeq ORF: 5058 bp
• Locus ID: 21872
• Cytogenetics: 7 35.02 cM
• MW: 189.3 kDa
• Gene Summary: Tjp1, TjpP2, and Tjp3 are closely related scaffolding proteins that link tight junction (TJ) transmembrane proteins such as claudins, junctional adhesion molecules, and occludin to the actin cytoskeleton (By similarity). The tight junction acts to limit movement of substances through the paracellular space and as a boundary between the compositionally distinct apical and basolateral plasma membrane domains of epithelial and endothelial cells. Necessary for lumenogenesis, and particularly efficient epithelial polarization and barrier formation (By similarity). Plays a role in the regulation of cell migration by targeting Cdc42bpb to the leading edge of migrating cells (By similarity). Plays an important role in podosome formation and associated function, thus regulating cell adhesion and matrix remodeling (By similarity). With Tjp2 and TJjp3, participates to the junctional retention and stability of the transcription factor Dbpa, but is not involved in its shuttling to the nucleus (By similarity).[UniProtKB/Swiss-Prot Function]