Aire (NM_009646) Mouse Tagged ORF Clone

CAT#: MR226851

Aire (Myc-DDK-tagged) - Mouse autoimmune regulator (autoimmune polyendocrinopathy candidiasis ectodermal dystrophy) (Aire)

Product Images

Circular map for MR226851

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Aire
• Synonyms: MGC123374; MGC123375
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR226851 representing NM_009646
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGGTGGGGATGGAATGCTACGCCGTCTGCTGAGGCTGCACCGCACCGAGATCGCGGTGGCCATAG
  ACAGTGCCTTTCCGCTGCTGCATGCTCTAGCCGACCACGACGTGGTCCCTGAGGACAAGTTCCAGGAGAC
  GCTCCGTCTGAAGGAGAAGGAAGGCTGCCCCCAGGCCTTCCACGCCCTGCTGTCCTGGCTCCTGACCCGG
  GACAGTGGGGCCATCCTGGATTTCTGGAGGATTCTCTTTAAGGACTACAATCTGGAGCGGTACAGCCGCC
  TGCATAGCATCCTGGACGGCTTCCCAAAAGATGTGGACCTAAACCAGTCCCGGAAAGGGAGAAAGCCCCT
  TGCTGGTCCCAAGGCCGCGGTACTGCCACCCAGACCCCCCACCAAGAGAAAAGCACTGGAGGAGCCTCGA
  GCCACCCCACCAGCAACTCTGGCCTCAAAGAGCGTCTCCAGCCCAGGCTCCCACCTGAAGACTAAGCCCC
  CTAAGAAGCCAGATGGCAACTTGGAGTCACAGCACCTTCCTCTTGGAAACGGAATTCAGACCATGGCAGC
  TTCTGTCCAGAGAGCTGTGACCGTGGCCTCTGGGGATGTTCCAGGAACCCGAGGGGCCGTGGAAGGGATC
  CTTATCCAGCAGGTGTTTGAGTCAGGAAGATCCAAGAAGTGCATTCAGGTTGGGGGAGAGTTTTATACAC
  CCAACAAGTTCGAAGACCCCAGTGGCAATTTGAAGAACAAGGCCCGGAGTGGTAGCAGCCTAAAGCCAGT
  GGTCCGAGCCAAGGGAGCCCAGGTCACTATACCTGGTAGAGATGAGCAGAAAGTGGGCCAGCAGTGTGGG
  GTTCCTCCCCTTCCATCCCTCCCCAGTGAGCCCCAGGTTAACCAGAACGAGGATGAGTGTGCCGTGTGCC
  ACGACGGAGGTGAGCTCATCTGTTGTGACGGCTGTCCCCGGGCCTTCCACCTGGCTTGCCTGTCCCCACC
  TCTGCAGGAGATCCCCAGTGGCCTCTGGAGATGCTCCTGCTGCCTCCAGGGCAGAGTCCAACAGAACCTG
  TCCCAGCCTGAGGTGTCCAGGCCCCCGGAGCTACCTGCAGAGACCCCGATCCTCGTGGGACTGAGGTCAG
  CTTCAGAGAAAACCAGGGGCCCATCCAGGGAGCTCAAAGCCAGCTCTGATGCTGCTGTCACATATGTGAA
  CCTGCTGGCCCCGCACCCTGCAGCTCCTCTGCTGGAGCCTTCAGCACTGTGCCCTCTACTGAGTGCTGGG
  AATGAGGGGCGGCCAGGTCCAGCACCAAGCGCGCGATGCAGTGTGTGTGGCGATGGCACCGAGGTGTTGC
  GGTGTGCACACTGTGCCGCTGCCTTCCACTGGCGCTGCCACTTCCCGACGGCCGCCGCCCGGCCGGGGAC
  CAATCTCCGCTGCAAATCCTGCTCTGCAGACTCGACTCCCACGCCAGGCACACCGGGCGAAGCTGTACCC
  ACCTCTGGGCCCCGTCCAGCACCTGGGCTTGCCAAGGTAGGGGACGACTCTGCTAGTCACGACCCTGTTC
  TACATAGGGACGACCTGGAGTCCCTCCTCAATGAGCACTCATTTGACGGCATCCTGCAGTGGGCCATCCA
  GAGCATGTCACGCCCGCTGGCCGAGACACCACCCTTCTCTTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR226851 representing NM_009646
  Red=Cloning site Green=Tags(s)
  
  MAGGDGMLRRLLRLHRTEIAVAIDSAFPLLHALADHDVVPEDKFQETLRLKEKEGCPQAFHALLSWLLTR
  DSGAILDFWRILFKDYNLERYSRLHSILDGFPKDVDLNQSRKGRKPLAGPKAAVLPPRPPTKRKALEEPR
  ATPPATLASKSVSSPGSHLKTKPPKKPDGNLESQHLPLGNGIQTMAASVQRAVTVASGDVPGTRGAVEGI
  LIQQVFESGRSKKCIQVGGEFYTPNKFEDPSGNLKNKARSGSSLKPVVRAKGAQVTIPGRDEQKVGQQCG
  VPPLPSLPSEPQVNQNEDECAVCHDGGELICCDGCPRAFHLACLSPPLQEIPSGLWRCSCCLQGRVQQNL
  SQPEVSRPPELPAETPILVGLRSASEKTRGPSRELKASSDAAVTYVNLLAPHPAAPLLEPSALCPLLSAG
  NEGRPGPAPSARCSVCGDGTEVLRCAHCAAAFHWRCHFPTAAARPGTNLRCKSCSADSTPTPGTPGEAVP
  TSGPRPAPGLAKVGDDSASHDPVLHRDDLESLLNEHSFDGILQWAIQSMSRPLAETPPFSS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009646
• ORF Size: 1653 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009646.1, NM_009646.2, NP_033776.1
• RefSeq Size: 1993 bp
• RefSeq ORF: 1659 bp
• Locus ID: 11634
• UniProt ID: Q9Z0E3
• Cytogenetics: 10 39.72 cM
• MW: 59.5 kDa
• Gene Summary: Transcription factor playing an essential role to promote self-tolerance in the thymus by regulating the expression of a wide array of self-antigens that have the commonality of being tissue-restricted in their expression pattern in the periphery, called tissue restricted antigens (TRA) (Probable). Binds to G-doublets in an A/T-rich environment; the preferred motif is a tandem repeat of 5'-. ATTGGTTA-3' combined with a 5'-TTATTA-3' box. Binds to nucleosomes (By similarity). Binds to chromatin and interacts selectively with histone H3 that is not methylated at 'Lys-4', not phosphorylated at 'Thr-3' and not methylated at 'Arg-2'. Functions as a sensor of histone H3 modifications that are important for the epigenetic regulation of gene expression. Mainly expressed by medullary thymic epithelial cells (mTECs), induces the expression of thousands of tissue-restricted proteins, which are presented on major histocompatibility complex class I (MHC-I) and MHC-II molecules to developing T-cells percolating through the thymic medulla (By similarity). Also induces self-tolerance through other mechanisms such as the regulation of the mTEC differentiation program (PubMed:19015306). Controls the medullary accumulation of thymic dendritic cells and the development of regulatory T-cell through the regulation of XCL1 expression (PubMed:21300913). Regulates the production of CCR4 and CCR7 ligands in medullary thymic epithelial cells and alters the coordinated maturation and migration of thymocytes (PubMed:19923453). In thimic B-cells, allows the presentation of licensing-dependent endogenous self-anitgen for negative selection (PubMed:26070482). In secondary lymphoid organs, induces functional inactivation of CD4(+) T-cells. Expressed by a distinct bone marrow-derived population, induces self-tolerance through a mechanism that does not require regulatory T-cells and is resitant to innate inflammatory stimuli (PubMed:23993652).[UniProtKB/Swiss-Prot Function]