Mov10 (NM_008619) Mouse Tagged ORF Clone

CAT#: MR226504

Mov10 (Myc-DDK-tagged) - Mouse Moloney leukemia virus 10 (Mov10), transcript variant 2

Product Images

Circular map for MR226504

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Mov10
• Synonyms: C77703; Mov-10
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR226504 representing NM_008619
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCTAGCAAGTTCAGCTGCCGAAAGCTCCGGGAGACCGGCCAGAGGTTCGAGAGTTTTCTGGCCGAAC
  GTGGACTGGACCTGGAGACAGATCGTGAGCGGCTGCGGACGATTTACAACCACGACTTCAAGCCCAGCTA
  TGGGACCCCTGCCCCTGGCTTCTCCTCCATGCTGTATGGAATGAAGATCGCAAATCTGGCCTTCGTCACC
  AAGACTCGGGTCAGGTTCTTCAAACTAGACCGCTGGGCTGATGTGCAGTTACCAGAAAAGAGGCGAATAA
  AGCCAGGGTCGAACATCAGCAAACAACACAGATCACTGTTGGCCAGGATCTTTCACGACAGGGCTGAGTA
  CCTTCATGGGAAGCATGGGGTAGACGTGGAGGTCCAGGGGCCCCATGAAGCCCGAGACGGGCAACTCCTT
  ATCCACCTGGATTTGAACCGCAAGGAGGTACTAACCCTACGGCTTCGGAACGGCGGAAGCAAACCTGTCA
  CCCTCACTCACCTATTCCCACTGTGCTGGACGCCCCAGTTTGTCTTCTACCATGGAGAACAGGACCTGCC
  CTGCCCACTGGGCCCCGGTGAAAGCTATGAACTCCACATCTACTGTAAGACCAGCATTGTGGGTTACTTC
  CCAGCCACTGTCCTCTGGGAACTCCTGGGACCCGGGGAGTCGGGAGCAGAAGGAGCAGAAACTTTCTACA
  TTGCCCGATTCCTGGCGGCTGTCGCCCACAGTCCCCTGGCTGCCCAGTTGAAACCCACAACTCCCTTCAA
  ACGCCCCCCTCGGCTCACCAGAAACTCTGTGTTGACCAACCGGATCGAGGAAGGAGAGAGGCCCGACCGA
  GCCAAGGGCTATGAACTAGAGCTAAGTTTGGCCCTGGGGACCTATTACCCACCCATCCTCCTCCGACAAC
  TGCTCCCTACCCTTCTTCAGGGACCAAGTATCTTCACTGCCCCAAAGGAGGTTGCTGAGATCAAGGCCCA
  GCTGGAGACAACCCTGAAATCCAGGAACTATGAGGTGAAACTCCGGCTGCTGCTGCACCTGGAAGAGCTG
  CAGATGGAGCATGACATCCGGCACTATGACCTGGACTCGGTACCCATGACCTGGGACCCTGTGGACCAGA
  ATCCCAGGCTGCTCACCCTGGAGGTTCCTGGTGTCGCAGAGAGCCGTCCCTCAGTGCTACGAGGTGACCA
  CCTTTTTGCCCTTTTGTCCTCTGAGACCCAACAGGACGACCCTGTCACCTACAAGGGTTTCGTGCACAAG
  GTGGAACTGGACCGTGTCAAGCTGAGCTTTTCTACAAGCCTCCTGAGCCGATTTGTGGATGGGCTGACCT
  TCAAGGTGAACTTTACCTTCAACCGCCAGCCCCTTCGGGTCCAGCACCGGGCCCTAGAGTTGACGGGGCG
  CTGGGTGCTATGGCCCATGCTTTTTCCTGTGGCCTCCCGTGGGGTCTCGTTGCTGCCCTCAGATGTGAAG
  TTCAAGCTGTACGATCGGAGTCTGGAGTCAAACCCTGAGCAACTGCAGGCCATGAAGCACATTGTGAGGG
  GTACCACCCGGCCTGCCCCCTACATCATCTTTGGGCCTCCAGGTACCGGCAAGACTGTCACATTAGTGGA
  GGCCATCAAACAGGTAGTGAAGCATTTGCCCAAAGCCCACATCCTGGCCTGTGCTCCATCCAACTCAGGG
  GCTGACCTCCTCTGTCAGCGGCTCCGGGTCCACCTGCCCAGCTCCATCTACCGTCTCCTGGCCCCCAGCA
  GGGACATCCGAATGGTGCCTGAGGACATTAAGACCTGCTGTAACTGGGATGCTAAGAAGGGAGAATATGT
  GTATCCTGCTAAGAAGCACCTGCAGCAATATCGGGTCTTAATTACCACCCTCATCACTGCCAGCAGGTTG
  GTGTCAGCCCAGTTTCCCATCGATCACTTCACACACATCTTCATCGATGAGGCTGGCCACTGCATGGAGC
  CTGAGAGTCTGGTGGCCATAGCAGGACTGATGGATGTCAAGGAAACGGGCAATCCCGGAGGGCAGCTGGT
  ACTGGCAGGAGACCCTCGGCAGCTGGGGCCTGTGCTGCGCTCGCCACTAGCCCTGAAGCATGGACTTGGC
  TACTCTCTGCTAGAGCGCCTGCTCGCCTACAACTCCTTGTATAAGAAGGGCCCCAATGGCTATGACCCCC
  AGTTCATCACCAAACTGCTCCGCAACTACAGGTCTCACCCCACCATCCTGGACATCCCTAACCAGCTGTA
  CTACGACGGAGAGCTGCAGGCCTGCGCAGATGTGGTGGATCGAGAACGGTTCTGCCGCTGGGAGGGGCTG
  CCTCAGCAGGGCTTCCCCATCATTTTCCATGGTGTAATGGGCAAAGATGAGAGAGAAGGCAATAGCCCAT
  CCTTCTTCAACCCTGAAGAGGCTGCTACAGTGACGTCATACCTGAAACAACTCCTGGCCCCTTCCTCCAA
  GAAGGGTAAAGCCCGCCTGAGCCCCCGAAATGTGGGCGTCATCTCCCCGTACCGGAAGCAGGTAGAAAAA
  ATCCGTTACTGCATCACAAAACTTGACCGAGAACTTCGCGGACTGGATGACATCAAAGATTTGAAGGTGG
  GCTCTGTGGAAGAGTTCCAAGGCCAAGAACGCAGCGTCATCCTCATCTCCACCGTCCGAAGCAGCCAGAG
  CTTTGTACAGCTGGATCTAGACTTTAACCTCGGTTTCCTTAAGAACCCCAAGAGGTTCAATGTTGCTGTG
  ACCCGAGCCAAGGCTTTGCTCATCGTAGTGGGCAACCCCCTCCTCCTAGGCCACGACCCAGACTGGAAAA
  CGTTCCTGGAGTTCTGTAAAGAAAACGGGGGATATACCGGGTGCCCCTTTCCTGCCAAACTGGACCTGCA
  GCAGGGACAGGACTTGCTCCAAGGTCTGAGCAAACTCAGCCCCTCTACCTCAGGGCCCCGCCGTCACCAG
  AATCTCCCCCAGGAGCGGGAGGGTGAAGGGGGCCTGCCCTTACAAGTGGAGCCAGAGTGGAGAAATGAGC
  TC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR226504 representing NM_008619
  Red=Cloning site Green=Tags(s)
  
  MPSKFSCRKLRETGQRFESFLAERGLDLETDRERLRTIYNHDFKPSYGTPAPGFSSMLYGMKIANLAFVT
  KTRVRFFKLDRWADVQLPEKRRIKPGSNISKQHRSLLARIFHDRAEYLHGKHGVDVEVQGPHEARDGQLL
  IHLDLNRKEVLTLRLRNGGSKPVTLTHLFPLCWTPQFVFYHGEQDLPCPLGPGESYELHIYCKTSIVGYF
  PATVLWELLGPGESGAEGAETFYIARFLAAVAHSPLAAQLKPTTPFKRPPRLTRNSVLTNRIEEGERPDR
  AKGYELELSLALGTYYPPILLRQLLPTLLQGPSIFTAPKEVAEIKAQLETTLKSRNYEVKLRLLLHLEEL
  QMEHDIRHYDLDSVPMTWDPVDQNPRLLTLEVPGVAESRPSVLRGDHLFALLSSETQQDDPVTYKGFVHK
  VELDRVKLSFSTSLLSRFVDGLTFKVNFTFNRQPLRVQHRALELTGRWVLWPMLFPVASRGVSLLPSDVK
  FKLYDRSLESNPEQLQAMKHIVRGTTRPAPYIIFGPPGTGKTVTLVEAIKQVVKHLPKAHILACAPSNSG
  ADLLCQRLRVHLPSSIYRLLAPSRDIRMVPEDIKTCCNWDAKKGEYVYPAKKHLQQYRVLITTLITASRL
  VSAQFPIDHFTHIFIDEAGHCMEPESLVAIAGLMDVKETGNPGGQLVLAGDPRQLGPVLRSPLALKHGLG
  YSLLERLLAYNSLYKKGPNGYDPQFITKLLRNYRSHPTILDIPNQLYYDGELQACADVVDRERFCRWEGL
  PQQGFPIIFHGVMGKDEREGNSPSFFNPEEAATVTSYLKQLLAPSSKKGKARLSPRNVGVISPYRKQVEK
  IRYCITKLDRELRGLDDIKDLKVGSVEEFQGQERSVILISTVRSSQSFVQLDLDFNLGFLKNPKRFNVAV
  TRAKALLIVVGNPLLLGHDPDWKTFLEFCKENGGYTGCPFPAKLDLQQGQDLLQGLSKLSPSTSGPRRHQ
  NLPQEREGEGGLPLQVEPEWRNEL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008619
• ORF Size: 3012 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008619.2, NP_032645.2
• RefSeq Size: 3616 bp
• RefSeq ORF: 3015 bp
• Locus ID: 17454
• UniProt ID: P23249
• Cytogenetics: 3 F2.2
• MW: 113.6 kDa
• Gene Summary: Probable RNA helicase. Required for miRNA-mediated gene silencing by the RNA-induced silencing complex (RISC). Required for both miRNA-mediated translational repression and miRNA-mediated cleavage of complementary mRNAs by RISC (By similarity). In cooperation with FMR1, regulates miRNA-mediated translational repression by AGO2 (By similarity). Restricts retrotransposition of long interspersed element-1 (LINE-1) in cooperation with TUT4 and TUT7 counteracting the RNA chaperonne activity of L1RE1. Facilitates LINE-1 uridylation by TUT4 and TUT7 (By similarity). Required for embryonic viability and for normal central nervous system development and function. Plays two critical roles in early brain development: suppresses retroelements in the nucleus by directly inhibiting cDNA synthesis, while regulates cytoskeletal mRNAs to influence neurite outgrowth in the cytosol (PubMed:28662698). May function as a messenger ribonucleoprotein (mRNP) clearance factor (By similarity).[UniProtKB/Swiss-Prot Function]