Gpr26 (NM_173410) Mouse Tagged ORF Clone

CAT#: MR224352

Gpr26 (Myc-DDK-tagged) - Mouse G protein-coupled receptor 26 (Gpr26)

Product Images

Circular map for MR224352

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Gpr26
• Synonyms: 9630036A11Rik; A730021D22
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR224352 representing NM_173410
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACTCGTGGGACGCGGGCCTGGCGGGGCTGCTGGTGGGCACTATCGGCGTGTCGCTGCTGTCCAACG
  GGCTGGTGCTGCTCTGCCTCCTGCACAGCGCTGACATCCGCCGCCAGGCGCCGGCGCTCTTCACTCTCAA
  CCTCACGTGTGGCAACCTGCTGTGTACCGTGGTCAACATGCCACTAACACTGGCCGGCGTCGTGGCACAA
  CGGCAGCCGGCCGGGGACCGCCTGTGCCGCCTGGCCGCCTTCCTCGACACCTTTCTGGCCGCCAACTCCA
  TGCTCAGCATGGCCGCGCTCAGCATCGACCGCTGGGTGGCTGTGGTCTTTCCGCTGAGCTACCGTGCCAA
  GATGCGCCTCCGAGATGCCGCCTTCATGGTGGCCTACACGTGGCTGCACGCGCTCACCTTCCCGGCCACC
  GCGCTCGCCCTGTCCTGGCTCGGCTTCCACCAGCTATATGCCTCGTGCACACTGTGCAGCCGGCGGCCGG
  ACGAGCGCCTGCGCTTTGCTGTCTTCACCAGCGCCTTCCATGCGCTCAGCTTCCTGCTCTCCTTCATCGT
  GCTCTGCTTCACGTACCTCAAGGTGCTCAAGGTGGCCCGCTTCCACTGCAAGCGCATCGACGTGATCACC
  ATGCAGACGCTTGTGCTGTTGGTGGACATACACCCCAGTGTGAGGGAACGGTGTCTGGAGGAACAGAAGC
  GGAGGCGACAGCGTGCCACCAAGAAGATCAGCACCTTCATAGGGACCTTCCTTGTGTGCTTTGCACCCTA
  TGTGATTACCAGGCTGGTGGAACTCTTCTCCACAGCACCCATTGGCTCTCACTGGGGAGTGCTGTCCAAG
  TGCTTGGCCTACAGCAAGGCCGCTTCTGACCCCTTCGTGTATTCCTTGCTGCGACACCAATACCGCAGGA
  GCTGCAAGGAGCTCCTGAACAGGATCTTCAACAGACGCTCCCTTCACTCTGTGGGCCTCACAGGTGACTC
  TCACAGCCAGAACATTCTGCCAGTGTCGGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR224352 representing NM_173410
  Red=Cloning site Green=Tags(s)
  
  MNSWDAGLAGLLVGTIGVSLLSNGLVLLCLLHSADIRRQAPALFTLNLTCGNLLCTVVNMPLTLAGVVAQ
  RQPAGDRLCRLAAFLDTFLAANSMLSMAALSIDRWVAVVFPLSYRAKMRLRDAAFMVAYTWLHALTFPAT
  ALALSWLGFHQLYASCTLCSRRPDERLRFAVFTSAFHALSFLLSFIVLCFTYLKVLKVARFHCKRIDVIT
  MQTLVLLVDIHPSVRERCLEEQKRRRQRATKKISTFIGTFLVCFAPYVITRLVELFSTAPIGSHWGVLSK
  CLAYSKAASDPFVYSLLRHQYRRSCKELLNRIFNRRSLHSVGLTGDSHSQNILPVSE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_173410
• ORF Size: 1011 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_173410.3, NP_775586.1
• RefSeq Size: 2799 bp
• RefSeq ORF: 1014 bp
• Locus ID: 233919
• UniProt ID: Q8BZA7
• Cytogenetics: 7 F3
• MW: 38.2 kDa
• Gene Summary: Orphan receptor. Displays a significant level of constitutive activity. Its effect is mediated by G(s)-alpha protein that stimulate adenylate cyclase, resulting in an elevation of intracellular cAMP (By similarity).[UniProtKB/Swiss-Prot Function]