Dhcr7 (NM_007856) Mouse Tagged ORF Clone

CAT#: MR223420

Dhcr7 (Myc-DDK-tagged) - Mouse 7-dehydrocholesterol reductase (Dhcr7)

Product Images

Circular map for MR223420

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Dhcr7
• Synonyms: AA409147
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR223420 representing NM_007856
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTTCGAAATCCCAGCACAACGCTCCCAAAGTCAAGAGTCCCAACGGCAAGGCTGGATCTCAAGGAC
  AATGGGGCCGTGCCTGGGAAGTGGACTGGTTCTCCCTGGCCAGCATCATTTTCCTGCTGCTCTTCGCTCC
  ATTCATTGTGTACTACTTCATCATGGCATGTGACCAGTACAGCTGCTCCCTGACTGCCCCCGCATTGGAT
  ATAGCTACAGGCCATGCTAGTCTGGCAGACATTTGGGCCAAGACACCACCTGTGACAGCTAAAGCTGCCC
  AACTGTATGCCTTGTGGGTCAGCTTCCAGGTGCTGCTTTATTCCTGGCTTCCTGACTTCTGCCACAGATT
  TCTGCCAGGTTATGTGGGAGGCGTCCAAGAAGGTGCCATTACTCCAGCAGGGGTTGTAAACAAGTATGAG
  GTGAATGGGCTGCAAGCCTGGCTCATTACCCACATCCTCTGGTTTGTGAACGCTTACCTCTTATCCTGGT
  TCTCCCCCACCATCATCTTTGACAACTGGATCCCCTTGCTATGGTGTGCCAACATCCTGGGCTATGCCGT
  GTCCACGTTCGCAATGATCAAGGGCTACCTGTTCCCCACCAGTGCCGAAGACTGCAAATTCACAGGCAAT
  TTCTTCTACAACTATATGATGGGAATTGAGTTCAACCCCCGCATCGGGAAGTGGTTTGACTTCAAGCTGT
  TCTTCAATGGACGACCAGGGATTGTAGCCTGGACCCTCATTAACCTGTCCTTCGCTGCCAAGCAGCAGGA
  GCTTTATGGCCATGTGACCAACTCCATGATTTTGGTCAATGTCTTGCAGGCCATCTACGTGTTAGACTTC
  TTCTGGAATGAAACCTGGTATCTGAAGACCATCGACATCTGCCATGACCACTTTGGGTGGTACCTAGGCT
  GGGGAGATTGTGTGTGGCTGCCCTACCTCTACACACTGCAGGGCCTGTACTTGGTGTACCACCCTGTGCA
  GCTCTCCACCCCCAATGCCTTGGGCATCCTGCTGCTTGGCCTCGTGGGTTACTATATCTTCCGAATGACC
  AACCATCAGAAGGACCTGTTCCGCAGGACAGATGGGCGCTGCCTCATCTGGGGCAAGAAGCCCAAGGCCA
  TTGAGTGCTCCTACACATCAGCTGATGGGCTGAAGCACCACAGCAAGCTGTTGGTCTCAGGCTTCTGGGG
  TGTGGCCCGCCACTTCAACTATACTGGTGACCTGATGGGCAGCCTTGCCTACTGCCTGGCTTGTGGCGGT
  GGCCACCTCCTCCCCTACTTTTACATCATCTATATGACTATCCTGCTCACCCACCGCTGCCTCCGTGATG
  AACACCGCTGTGCCAACAAGTATGGCCGTGATTGGGAGCGCTACACAGCCGCTGTGCCCTACCGCCTGCT
  GCCTGGAATCTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR223420 representing NM_007856
  Red=Cloning site Green=Tags(s)
  
  MASKSQHNAPKVKSPNGKAGSQGQWGRAWEVDWFSLASIIFLLLFAPFIVYYFIMACDQYSCSLTAPALD
  IATGHASLADIWAKTPPVTAKAAQLYALWVSFQVLLYSWLPDFCHRFLPGYVGGVQEGAITPAGVVNKYE
  VNGLQAWLITHILWFVNAYLLSWFSPTIIFDNWIPLLWCANILGYAVSTFAMIKGYLFPTSAEDCKFTGN
  FFYNYMMGIEFNPRIGKWFDFKLFFNGRPGIVAWTLINLSFAAKQQELYGHVTNSMILVNVLQAIYVLDF
  FWNETWYLKTIDICHDHFGWYLGWGDCVWLPYLYTLQGLYLVYHPVQLSTPNALGILLLGLVGYYIFRMT
  NHQKDLFRRTDGRCLIWGKKPKAIECSYTSADGLKHHSKLLVSGFWGVARHFNYTGDLMGSLAYCLACGG
  GHLLPYFYIIYMTILLTHRCLRDEHRCANKYGRDWERYTAAVPYRLLPGIF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007856
• ORF Size: 1413 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007856.2, NP_031882.1
• RefSeq Size: 2845 bp
• RefSeq ORF: 1416 bp
• Locus ID: 13360
• UniProt ID: O88455
• Cytogenetics: 7 F5
• MW: 54.4 kDa
• Gene Summary: Production of cholesterol by reduction of C7-C8 double bond of 7-dehydrocholesterol (7-DHC).[UniProtKB/Swiss-Prot Function]