Nrcam (NM_176930) Mouse Tagged ORF Clone

CAT#: MR219511

Nrcam (Myc-DDK-tagged) - Mouse neuron-glia-CAM-related cell adhesion molecule (Nrcam), transcript variant 1

Product Images

Circular map for MR219511

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Nrcam
• Synonyms: Bravo; C030017F07Rik; C130076O07Rik; mKIAA0343
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR219511 representing NM_176930
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGCTTAAAATAATGCCGAAGAAGAAGCACTTATCTGCAGGCGGAGTGCCCCTGATTCTCTTCCTGT
  GCCAGATGATCAGCGCGCTGGATGTTCCTCTCGATCTGGTACAACCTCCAACCATCACTCAACAGTCACC
  AAAAGACTACATCATTGACCCTCGGGAGAATATTGTAATCCAGTGTGAGGCCAAAGGGAAACCTCCCCCA
  AGCTTTTCCTGGACTCGTAATGGAACTCACTTTGACATAGACAAGGACCCTCTGGTCACCATGAAGCCTG
  GCTCAGGAACCCTTGTCATCAACATCATGAGTGAGGGGAAGGCAGAGACCTATGAAGGAGTCTACCAGTG
  CACTGCAAGGAACGAGCGTGGAGCTGCCGTCTCCAATAACATTGTTGTCCGCCCATCTAGGTCACCCTTG
  TGGACCAAGGAAAGACTTGAACCTATAGTACTCCAGAATGGGCAGTCATTAGTACTTCCATGTAGGCCTC
  CGATTGGATTACCTCCGGCCATAATATTTTGGATGGATAATTCCTTTCAGAGACTTCCACAGAGTGAGCG
  GGTTTCCCAAGGCCTAAATGGAGACCTTTACTTCTCCAATGTCCTCCCAGAGGACACCCGTGAGGACTAT
  ATCTGCTATGCCAGATTTAATCACACTCAAACCATCCAGCAGAAGCAGCCTATTTCTTTGAAGGTGATTT
  CAGTGGATGAATTGAATGACACTATAGCTGCTAATTTGAGTGACACTGAGTTTTATGGTGCTAAATCTAG
  TAAAGAGAGGCCCCCAACGTTTCTAACTCCAGAGGGCAATGAAAGCCACAAAGAGGAATTAAGAGGAAAC
  GTGCTTTCGCTGGAGTGCATTGCGGAAGGCCTACCTACTCCAATTATTTACTGGATCAAAGAAGACGGAA
  TGCTTCCCGCCAACCGGACATTTTATCGGAACTTTAAGAAAACCCTGCAGATCACTCATGTTTCCGAAGC
  CGACTCTGGAAATTATCAGTGCATAGCAAAAAACGCATTAGGAGCCGTCCATCACACCATTTCTGTCACT
  GTTAAAGCGGCTCCCTACTGGATCGTGGCACCTCAAAACCTCGTGCTTTCCCCGGGAGAGAATGGGACCC
  TCATCTGCAGAGCTAATGGCAACCCGAAACCCAGAATTAGCTGGTTAACAAATGGAGTCCCAATAGAAAT
  TGCTCTCGATGACCCCAGCAGAAAAATAGATGGCGATACCATTATATTTTCAAATGTTCAAGAAAGCTCA
  AGTGCGGTTTATCAGTGCAATGCCTCTAACAAATATGGATATTTACTAGCAAATGCATTTGTAAATGTTC
  TCGCTGAACCACCTCGGATTCTCACATCAGCAAACACACTGTACCAGGTCATTGCAAACAGGCCTGCTTT
  GCTAGATTGTGCCTTCTTTGGATCTCCTATGCCTACCATTGAGTGGTTTAAAGGCACTAAAGGAAGCGCT
  CTTCATGAAGACATTTATGTTTTGCATGATAATGGAACATTAGAAATTCCCGTGGCCCAAAAGGATAGTA
  CAGGGACGTATACTTGTGTCGCACGGAATAAACTAGGGATGGCAAAGAATGAAGTTCACTTGGAAATCAA
  AGATCCAACCAGGATCATTAAACAACCTGAGTATGCAGTCGTCCAGAGGGGGAGCAAGGTGTCCTTTGAA
  TGCAGAGTGAAACATGACCACACCTTAATCCCCACCATTATGTGGCTGAAGGACAATGGAGAGCTGCCCA
  ATGATGAAAGGTTCTCCACTGACAAGGATCATCTGGTGGTATCTGATGTAAAGGATGACGATGGCGGAAC
  CTACACGTGTACGGCCAACACAACGCTGGACAGTGCTTCGGCCAGCGCTGTGCTCAGGGTTGTCGCTCCT
  ACTCCAACTCCAGCCCCCATTTACGATGTCCCGAATCCTCCCTTTGATTTAGAATTGACCAATCAACTTG
  ACAAAAGTGTTCAGCTGACATGGACCCCAGGCGACGACAACAATAGCCCCATTACAAAATTCATCATTGA
  GTATGAAGATGCAATGCATGATGCAGGGCTGTGGCGCCACCAGGCTGAAGTTTCTGGAACACAGACCACA
  GCCCAACTGAAGCTGTCTCCCTATGTGAACTACTCCTTCCGTGTCATGGCAGAGAACAGCATTGGGAGAA
  GTATGCCGAGCGAGGCATCCGAGCAGTATCTTACAAAAGCCGCAGAACCAGATCAGAATCCCATGGCTGT
  GGAAGGACTAGGGACAGAGCCGGACAACTTGGTGATTACATGGAAGCCCCTGAATGGTTTTCAATCGAAT
  GGGCCTGGCCTCCAGTACAAAGTGAGCTGGCGCCAGAAAGATGGTGACGATGAGTGGACGTCTGTGGTTG
  TGGCCAATGTATCCAAATACATTGTTTCTGGCACACCAACCTTTGTCCCATACCTGATAAAAGTTCAAGC
  TCTGAATGATGTGGGGTTTGCACCAGAGCCAGCTGCAGTCATGGGGCATTCTGGAGAAGACCTTCCAATG
  GTGGCTCCTGGAAATGTTCGCGTCAGCGTGGTGAACAGTACGCTGGCAGAGGTGCACTGGGACCCAGTTC
  CTCCCAAGAGTGTCCGAGGACACTTACAAGGCTACCGGATTTACTACTGGAAGACCCAGAGCTCCTCTAA
  AAGAAACAGGCGCCACATTGAGAAGAAGATCCTCACCTTCCAGGGCACCAAGACTCACGGCATGCTGCCA
  GGGCTGCAGCCATACAGTCACTATGCCCTCAACGTCCGAGTGGTCAACGGGAAAGGGGAGGGCCCAGCCA
  GCACGGACAGAGGCTTCCATACACCGGAGGGAGTCCCTAGTGCTCCCTCATCTTTGAAAATTGTTAATCC
  CACACTGGACTCCCTCACTTTGGAATGGGACCCCCCAAGCCACCCAAATGGCATCCTGACTGAGTACATC
  TTACAATATCAGCCAATTAACAGCACACATGAGCTAGGCCCTCTGGTAGATTTAAAAATTCCTGCCAACA
  AGACCCGCTGGACTTTGAAAAATTTAAATTTCAGCACTCGGTACAAGTTCTATTTCTATGCACAGACATC
  AGTGGGGCCAGGCAGTCAGATCACAGAGGAAGCGATAACGACTGTGGACGAAGCTGGTATTCCTCCACCT
  GATGTAGGTGCAGGCAAAGGCAAAGAAGAATGGAGGAAAGAAATTGTAAATGGTTCTCGAAGCTTCTTTG
  GGTTAAAGGGTCTAATGCCAGGAACAGCATACAAAGTTCGAGTTGGTGCTGAGGGGGACTCTGGTTTTGT
  GAGTTCAGAGGATGTGTTTGAGACAGGACCAGCAATGGCAAGCCGGCAAGTGGATATCGCGACCCAAGGC
  TGGTTCATAGGTCTAATGTGCGCTGTTGCTCTCCTCATCTTAATTTTGCTGATTGTTTGCTTCATCAGAA
  GAAACAAAGGTGGTAAATACCCAGTTAAAGAAAAGGAGGATGCTCATGCAGACCCTGAAATCCAGCCCAT
  GAAGGAAGATGATGGGACGTTTGGAGAATACAGTGATGCAGAAGATCACAAGCCTTTGAAAAAAGGAAGT
  CGAACACCTTCAGACAGGACTGTGAAAAAAGAAGATAGCGATGATAGTCTGGTTGACTATGGAGAGGGGG
  TGAACGGCCAATTCAACGAGGATGGCTCCTTTATTGGCCAATACAGTGGTAAGAAAGAGAAAGAGCCAGC
  AGAGGGAAATGAAAGCTCAGAGGCCCCTTCTCCTGTCAACGCAATGAACTCCTTTGTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR219511 representing NM_176930
  Red=Cloning site Green=Tags(s)
  
  MQLKIMPKKKHLSAGGVPLILFLCQMISALDVPLDLVQPPTITQQSPKDYIIDPRENIVIQCEAKGKPPP
  SFSWTRNGTHFDIDKDPLVTMKPGSGTLVINIMSEGKAETYEGVYQCTARNERGAAVSNNIVVRPSRSPL
  WTKERLEPIVLQNGQSLVLPCRPPIGLPPAIIFWMDNSFQRLPQSERVSQGLNGDLYFSNVLPEDTREDY
  ICYARFNHTQTIQQKQPISLKVISVDELNDTIAANLSDTEFYGAKSSKERPPTFLTPEGNESHKEELRGN
  VLSLECIAEGLPTPIIYWIKEDGMLPANRTFYRNFKKTLQITHVSEADSGNYQCIAKNALGAVHHTISVT
  VKAAPYWIVAPQNLVLSPGENGTLICRANGNPKPRISWLTNGVPIEIALDDPSRKIDGDTIIFSNVQESS
  SAVYQCNASNKYGYLLANAFVNVLAEPPRILTSANTLYQVIANRPALLDCAFFGSPMPTIEWFKGTKGSA
  LHEDIYVLHDNGTLEIPVAQKDSTGTYTCVARNKLGMAKNEVHLEIKDPTRIIKQPEYAVVQRGSKVSFE
  CRVKHDHTLIPTIMWLKDNGELPNDERFSTDKDHLVVSDVKDDDGGTYTCTANTTLDSASASAVLRVVAP
  TPTPAPIYDVPNPPFDLELTNQLDKSVQLTWTPGDDNNSPITKFIIEYEDAMHDAGLWRHQAEVSGTQTT
  AQLKLSPYVNYSFRVMAENSIGRSMPSEASEQYLTKAAEPDQNPMAVEGLGTEPDNLVITWKPLNGFQSN
  GPGLQYKVSWRQKDGDDEWTSVVVANVSKYIVSGTPTFVPYLIKVQALNDVGFAPEPAAVMGHSGEDLPM
  VAPGNVRVSVVNSTLAEVHWDPVPPKSVRGHLQGYRIYYWKTQSSSKRNRRHIEKKILTFQGTKTHGMLP
  GLQPYSHYALNVRVVNGKGEGPASTDRGFHTPEGVPSAPSSLKIVNPTLDSLTLEWDPPSHPNGILTEYI
  LQYQPINSTHELGPLVDLKIPANKTRWTLKNLNFSTRYKFYFYAQTSVGPGSQITEEAITTVDEAGIPPP
  DVGAGKGKEEWRKEIVNGSRSFFGLKGLMPGTAYKVRVGAEGDSGFVSSEDVFETGPAMASRQVDIATQG
  WFIGLMCAVALLILILLIVCFIRRNKGGKYPVKEKEDAHADPEIQPMKEDDGTFGEYSDAEDHKPLKKGS
  RTPSDRTVKKEDSDDSLVDYGEGVNGQFNEDGSFIGQYSGKKEKEPAEGNESSEAPSPVNAMNSFV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_176930
• ORF Size: 3768 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_176930.4, NP_795904.3
• RefSeq Size: 7572 bp
• RefSeq ORF: 3771 bp
• Locus ID: 319504
• UniProt ID: Q810U4
• Cytogenetics: 12 20.71 cM
• MW: 139 kDa
• Gene Summary: Cell adhesion protein that is required for normal responses to cell-cell contacts in brain and in the peripheral nervous system. Plays a role in neurite outgrowth in response to contactin binding (PubMed:11564762). Plays a role in mediating cell-cell contacts between Schwann cells and axons (PubMed:20188654). Plays a role in the formation and maintenance of the nodes of Ranvier on myelinated axons. Nodes of Ranvier contain clustered sodium channels that are crucial for the saltatory propagation of action potentials along myelinated axons. During development, nodes of Ranvier are formed by the fusion of two heminodes. Required for normal clustering of sodium channels at heminodes; not required for the formation of mature nodes with normal sodium channel clusters (PubMed:14602817, PubMed:20188654). Required, together with GLDN, for maintaining NFASC and sodium channel clusters at mature nodes of Ranvier (PubMed:24719088).[UniProtKB/Swiss-Prot Function]