Ctnna2 (NM_009819) Mouse Tagged ORF Clone

CAT#: MR218524

Ctnna2 (Myc-DDK-tagged) - Mouse catenin (cadherin associated protein), alpha 2 (Ctnna2), transcript variant 2

Product Images

Circular map for MR218524

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ctnna2
• Synonyms: AI481747; Catna; Catna2; cdf; chp
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR218524 representing NM_009819
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACTTCGGCAACTTCACCTATTATTTTAAAATGGGATCCCAAAAGTTTGGAAATCCGGACACTCACAG
  TGGAAAGACTATTGGAGCCACTTGTGACACAGGTGACAACACTTGTCAACACAAGCAACAAAGGTCCGTC
  TGGTAAAAAGAAAGGGAGGTCAAAGAAAGCCCATGTGCTGGCAGCATCTGTAGAACAAGCTACTCAGAAC
  TTCCTGGAAAAGGGTGAACAGATCGCTAAGGAGAGCCAAGACCTCAAAGAAGAGTTAGTGGCTGCTGTAG
  AGGATGTGCGGAAGCAAGGTGAGACAATGCGGATTGCCTCCTCAGAGTTTGCAGATGACCCTTGCTCTTC
  TGTCAAGCGTGGCACCATGGTGCGTGCAGCACGGGCTCTGCTATCGGCTGTGACACGCTTGCTCATCCTG
  GCCGACATGGCAGATGTCATGAGGCTTTTATCGCATCTGAAAATTGTCGAGGAGGCCTTGGAAGCAGTCA
  AAAATGCCACAAATGAACAAGACCTTGCAAACCGATTTAAAGAGTTTGGGAAAGAGATGGTGAAACTGAA
  CTATGTAGCAGCAAGACGGCAGCAGGAGCTCAAGGACCCTCACTGTAGGGATGAGATGGCTGCAGCCCGT
  GGAGCCCTGAAGAAGAATGCCACCATGCTGTACACAGCCTCCCAAGCCTTCCTCCGGCATCCAGATGTTG
  CTGCTACAAGAGCCAACCGAGATTATGTATTTAAACAAGTCCAAGAGGCCATAGCTGGCATCTCCAGTGC
  TGCTCAGGCCACCTCCCCCACCGATGAAGCCAAAGGCCACACAGGCATCGGCGAGCTGGCTGCAGCCCTG
  AATGAGTTTGATAATAAGATCATCCTGGACCCCATGACATTCAGCGAGGCCAGGTTCCGACCATCCCTGG
  AGGAGAGACTGGAGAGCATCATCAGTGGGGCTGCTCTCATGGCAGATTCCTCCTGCACACGTGATGACCG
  CCGTGAGCGTATTGTGGCCGAGTGCAATGCAGTTCGACAGGCACTCCAGGACCTGCTAAGCGAGTACATG
  AATAACACTGGAAGGAAAGAGAAAGGAGACCCTCTCAACATTGCGATTGACAAGATGACCAAGAAAACAA
  GAGATCTGAGGAGACAGCTTCGGAAAGCTGTGATGGATCACATCTCAGATTCTTTCTTGGAAACCAATGT
  CCCCTTGCTGGTTCTCATTGAGGCTGCGAAGAGCGGGAATGAGAAGGAGGTGAAGGAATACGCCCAAGTT
  TTCCGTGAACATGCCAACAAGCTGGTGGAGGTTGCCAATTTGGCTTGTTCCATCTCCAACAATGAGGAAG
  GGGTGAAATTAGTCAGAATGGCAGCCACCCAGATTGACAGCCTGTGTCCCCAAGTCATTAATGCTGCCCT
  CACACTGGCTGCTCGGCCACAGAGTAAAGTTGCTCAGGACAACATGGATGTCTTCAAAGACCAGTGGGAA
  AAGCAAGTCCGTGTGCTCACTGAGGCAGTGGATGACATCACCTCTGTGGATGACTTCCTCTCTGTCTCAG
  AAAACCATATCTTGGAGGATGTGAACAAATGTGTGATTGCCCTGCAAGAGGGAGATGTGGACACACTGGA
  TCGCACAGCTGGGGCCATACGGGGCCGGGCAGCCCGGGTCATTCACATCATCAATGCAGAGATGGAGAAC
  TATGAAGCTGGGGTCTATACAGAGAAAGTGCTGGAAGCCACAAAATTGCTTTCAGAAACAGTGATGCCAC
  GCTTTGCTGAACAAGTTGAGGTGGCCATCGAAGCCCTGAGCGCCAATGTCCCTCAGCCATTCGAGGAGAA
  CGAGTTCATCGATGCCTCGCGCCTGGTGTATGACGGTGTTCGGGACATCAGAAAGGCTGTGCTGATGATC
  AGGACTCCAGAAGAGCTAGAAGATGATTCCGACTTTGAGCAAGAGGATTATGATGTGCGGAGTCGGACAA
  GTGTTCAGACAGAGGACGACCAGCTCATTGCTGGACAGAGTGCACGGGCCATCATGGCGCAACTACCACA
  GGAGGAGAAAGCAAAAATAGCTGAACAGGTGGAGATTTTCCACCAAGAAAAAAGCAAGCTGGATGCTGAA
  GTGGCCAAGTGGGATGACAGCGGCAATGACATCATTGTGCTGGCCAAGCAGATGTGTATGATCATGATGG
  AGATGACAGACTTCACAAGAGGCAAAGGCCCACTGAAAAATACATCTGATGTCATTAATGCTGCCAAGAA
  GATTGCAGAAGCAGGCTCTCGAATGGACAAATTAGCGCGCGCTGTGGCTGATCAGTGTCCTGATTCAGCA
  TGTAAGCAGGATTTATTAGCCTACCTTCAGCGGATTGCTTTGTACTGCCATCAGCTTAACATCTGCAGCA
  AAGTGAAGGCCGAGGTTCAGAACCTAGGAGGAGAGCTCATTGTGTCAGGGACAGGAGTTCAGAGCACTTT
  CACTACCTTTTATGAGGTAGATTGTGATGTCATAGATGGGGGCAGGGCTAGTCAACTTTCTACCCACCTC
  CCAACCTGTGCTGAGGGAGCTCCAATCGGGAGTGGAAGCAGTGACTCCTCCATGCTGGACAGTGCTACAT
  CACTCATCCAGGCAGCCAAAAACCTGATGAATGCTGTTGTCCTCACGGTGAAAGCGTCTTATGTAGCCTC
  AACTAAATACCAGAAGGTCTATGGAACAGCAGCTGTCAACTCTCCAGTTGTGTCTTGGAAGATGAAGGCT
  CCTGAAAAGAAGCCCCTTGTGAAGAGAGAAAAGCCTGAAGAATTCCAGACAAGAGTTAGACGGGGGTCTC
  AAAAGAAACACATTTCACCTGTGCAGGCTTTAAGTGAATTCAAGGCAATGGATTCCTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR218524 representing NM_009819
  Red=Cloning site Green=Tags(s)
  
  MTSATSPIILKWDPKSLEIRTLTVERLLEPLVTQVTTLVNTSNKGPSGKKKGRSKKAHVLAASVEQATQN
  FLEKGEQIAKESQDLKEELVAAVEDVRKQGETMRIASSEFADDPCSSVKRGTMVRAARALLSAVTRLLIL
  ADMADVMRLLSHLKIVEEALEAVKNATNEQDLANRFKEFGKEMVKLNYVAARRQQELKDPHCRDEMAAAR
  GALKKNATMLYTASQAFLRHPDVAATRANRDYVFKQVQEAIAGISSAAQATSPTDEAKGHTGIGELAAAL
  NEFDNKIILDPMTFSEARFRPSLEERLESIISGAALMADSSCTRDDRRERIVAECNAVRQALQDLLSEYM
  NNTGRKEKGDPLNIAIDKMTKKTRDLRRQLRKAVMDHISDSFLETNVPLLVLIEAAKSGNEKEVKEYAQV
  FREHANKLVEVANLACSISNNEEGVKLVRMAATQIDSLCPQVINAALTLAARPQSKVAQDNMDVFKDQWE
  KQVRVLTEAVDDITSVDDFLSVSENHILEDVNKCVIALQEGDVDTLDRTAGAIRGRAARVIHIINAEMEN
  YEAGVYTEKVLEATKLLSETVMPRFAEQVEVAIEALSANVPQPFEENEFIDASRLVYDGVRDIRKAVLMI
  RTPEELEDDSDFEQEDYDVRSRTSVQTEDDQLIAGQSARAIMAQLPQEEKAKIAEQVEIFHQEKSKLDAE
  VAKWDDSGNDIIVLAKQMCMIMMEMTDFTRGKGPLKNTSDVINAAKKIAEAGSRMDKLARAVADQCPDSA
  CKQDLLAYLQRIALYCHQLNICSKVKAEVQNLGGELIVSGTGVQSTFTTFYEVDCDVIDGGRASQLSTHL
  PTCAEGAPIGSGSSDSSMLDSATSLIQAAKNLMNAVVLTVKASYVASTKYQKVYGTAAVNSPVVSWKMKA
  PEKKPLVKREKPEEFQTRVRRGSQKKHISPVQALSEFKAMDSF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009819
• ORF Size: 2859 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009819.2, NP_033949.2
• RefSeq Size: 4046 bp
• RefSeq ORF: 2862 bp
• Locus ID: 12386
• UniProt ID: Q61301
• Cytogenetics: 6 33.54 cM
• MW: 105.7 kDa
• Gene Summary: May function as a linker between cadherin adhesion receptors and the cytoskeleton to regulate cell-cell adhesion and differentiation in the nervous system (PubMed:12123610, PubMed:15034585). Required for proper regulation of cortical neuronal migration and neurite growth. It acts as negative regulator of Arp2/3 complex activity and Arp2/3-mediated actin polymerization. It thereby suppresses excessive actin branching which would impair neurite growth and stability (By similarity). Regulates morphological plasticity of synapses and cerebellar and hippocampal lamination during development. Functions in the control of startle modulation (PubMed:12089526).[UniProtKB/Swiss-Prot Function]