Drosha (NM_026799) Mouse Tagged ORF Clone

CAT#: MR211952

Drosha (Myc-DDK-tagged) - Mouse drosha, ribonuclease type III (Drosha), transcript variant 2

Product Images

Circular map for MR211952

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Drosha
• Synonyms: 1110013A17Rik; AI874853; Etohi2; Rn3; Rnasen
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR211952 representing NM_026799
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAAGGCAATACGTGTCATAGAATGTCGTACCACCCAGGACGAGGGTGTCCCCGGGGCCGAGGAGGAC
  ACGGAGCCAGACCTTCAGCACCAGCTTTCAGACCCCAAAACCTGCGACTTCTTCATCCCCAGCAGCCGCC
  TGCGCAGTATCAATATGAGCCTCCGAGCGCCCCCTCTTCCTCCTACTCGAACTCTCAGGCCCCCAGCTTT
  ATGCCCCCACGGCCAGACTTTGTCCCTTACCCTCCCCCAGCGGCGCCGTCTGCCCAAGGGCCTCTCCCTC
  CCTGCCCAGTGAGGCCGCCTTACCCCAACCACCAGATGAGACACCCTTTCCCGGTGCCTCCCTGTTTTCC
  ACCCATGCCCCCTCCGATGCCTTGTCCCAATAACCCGCCTGCCTCCGGAGCACCTCCCGGACAAGGCACT
  TTCCCCTTCATGGTGCCCCCTCCTTCCATGCCCCACCCTCCGCCCCCGCCCGTCATGCCGCAGCAGGTTA
  ATTACCAGTACCCCCCTGGGTACTCGCACAGCTTCCCACCGCCCGGCTTCAACAGTTACCAGAACAACTC
  CAGCTCTTTCCCACCCAGTGCTAACAGCAGCAGCACTCCTCATTTTCGACACCTCCCACCATACTCACTC
  CCAAAGGCTCAGAATGAGAGGCGGTCCCCAGAAAGGCTCAAGCACTACGACGACCACAGGCACCGAGATC
  ACAGTCACGGGCGAGGCGAGAGGCATCGGTCCCTGGAGCGCAGGGAGCGCGGCCGCAGCCCTGAAAGGAG
  AAGACCTGAGAGCCGCTACCGCTCAGACTATGATCGGGGGAGAACGCCACCGCCTCGCCACCGCAGCTAT
  GAAAGGAGCAGAGAGCGGGATCGAGAGAGACACAGGCACCGGGAGGCCCGCAGATCACCGTCTCTAGAAA
  GGTCCTACAAGAAAGAGTATAAGAGATCTGGAAGGAGTTACGCTTTACCAGTTGCTCCTGAGCCCGCTGG
  GTGCACACCAGAGTTGCCTGGGGAGATGATTAAAACTACAGAGTCTTGGGCCCCGCCCCCGGAGAATGTG
  AATCATCGTTCTCCAAGCAGGGAGAAGAAGAGAGCTCGTTGGGAGGAGGAAAAAGACAGATGGAGCGACA
  GCCAGGGCTCTGGCAAAGAGAAGAACTACACGTCCATCAAAGAGAAAGAGGCAGAGGAGGTGCCTCCAGA
  GAAGACGGAGGAGGAGGAGGAAGAGCTCCTTAAGCCTGTGTGGATTCGCTGTACACATTCGGAAAGCTAT
  TACTCCAGTGACCCAATGGATCAGGTGGGAGACTCGACTGTCGTAGGGACAAGCAGGCTCCGTGATTTGT
  ATGACAAATTTGAGGAAGAATTGGGGAATAGGCAAGAGAAGGCCAAAGCTGCCCGGCCTCCGTGGGAGCC
  TCCGAAGACAAAGCTGGATGAAGATTTAGAGAGTTCCAGTGAGTCTGAATGTGAGACTGACGACGACAGC
  ACCTGTTCGAGCAGCTCGGACTCGGAGGTGTTCGATGTCATTGCAGAGATTAAACGCAAAAAGGCTCACC
  CTGACCGGCTTCATGATGAACTCTGGTACAACGACCCAGGCCAGATGAACGATGGACCGCTTTGCAAATG
  CAGTGCAAAAGCCAGACGCACAGGAATCCGCCACAGCATTTATCCCGGAGAAGAGGCAATCAAGCCCTGC
  CGTCCGATGACCAACAACGCTGGCCGGCTTTTCCACTATCGGATCACCGTCTCCCCGCCTACCAACTTCT
  TAACTGACAGGCCAACAGTCATAGAATATGATGACCACGAGTATATTTTTGAAGGATTTTCTATGTTTGC
  ACATGCTCCTCTGACCAATATTCCACTGTGTAAAGTGATTCGATTCAACATAGACTACACGATTCATTTT
  ATCGAAGAGATGATGCCTGAGAATTTTTGTGTGAAAGGACTTGAACTGTTTTCATTGTTCCTATTCAGAG
  ATATTTTGGAATTATATGACTGGAATCTTAAAGGTCCTTTGTTTGAAGACAGCCCTCCCTGCTGTCCGAG
  ATTTCATTTCATGCCACGTTTTGTAAGATTTCTTCCAGATGGAGGCAAAGAAGTGTTATCCATGCACCAG
  ATCCTTCTCTACCTGCTGCGCTGCAGCAAGGCTCTGGTGCCCGAGGAGGAGATTGCCAACATGCTCCAGT
  GGGAGGAGCTCGAGTGGCAGAAATACGCAGAGGAGTGCAAAGGCATGATCGTTACCAACCCTGGGACGAA
  ACCGAGCTCTGTCCGCATTGATCAGCTGGATCGTGAACAGTTCAACCCCGAAGTGATCACTTTCCCGATT
  ATCGTTCACTTTGGGATCCGCCCCGCACAGTTGAGTTATGCAGGAGACCCACAGTATCAGAAATTATGGA
  AAAGCTATGTGAAACTTCGCCACCTCCTAGCAAACAGTCCCAAAGTCAAACAAACTGACAAGCAGAAGCT
  GGCTCAGAGGGAGGAAGCGCTCCAGAAAATACGGCAGAAGAACACGATGCGGCGAGAAGTCACCGTGGAG
  CTGAGTAGCCAAGGATTCTGGAAAACTGGCATTCGTTCTGATGTCTGTCAGCATGCAATGATGCTGCCTG
  TTCTGACCCATCACATCCGGTACCATCAATGCTTAATGCACCTGGACAAGTTGATAGGATATACTTTCCA
  AGACCGTTGTCTGCTCCAGCTTGCCATGACTCATCCGAGTCACCATTTAAATTTTGGAATGAATCCTGAC
  CATGCCAGGAATTCTTTGTCTAACTGTGGAATTCGACAACCCAAATATGGAGACAGAAAAGTTCATCACA
  TGCACATGCGGAAAAAAGGAATTAATACCCTAATAAATATCATGTCACGCCTTGGCCAAGATGATCCAAC
  TCCTTCAAGGATTAATCACAACGAAAGGTTGGAGTTCCTGGGGGATGCTGTTGTTGAGTTTCTGACCAGT
  GTCCACCTGTACTACCTGTTTCCTAGCTTGGAGGAAGGGGGCTTGGCGACCTATCGGACCGCCATTGTTC
  AGAATCAGCACCTCGCCATGCTTGCAAAGAAACTTGAACTGGATCGATTTATGCTATATGCCCATGGGCC
  CGACCTATGTAGAGAATCAGATCTCCGCCATGCGATGGCCAATTGTTTTGAAGCCTTGATAGGAGCTGTT
  TACTTGGAGGGGAGCCTAGAGGAAGCCAAACAGTTATTTGGACGCTTACTCTTTAATGATCCGGACCTTC
  GAGAAGTCTGGCTCAATTATCCTCTCCACCCACTCCAACTACAAGAGCCAAATACGGATCGGCAACTTAT
  TGAAACTTCCCCAGTTCTACAGAAACTTACGGAGTTTGAAGAAGCAATTGGAGTGATCTTCACTCACGTC
  CGGCTTCTGGCGAGGGCGTTCACACTGAGAACCGTGGGCTTTAACCACCTGACCCTAGGCCACAATCAGA
  GGATGGAATTTCTGGGCGACTCCATAATGCAGCTGGTGGCCACAGAGTACTTGTTCATTCATTTCCCGGA
  CCATCACGAAGGACACTTGACGTTGTTACGAAGTTCCTTAGTGAACAACAGAACGCAAGCCAAGGTAGCA
  GAGGAACTGGGCATGCAGGAGTATGCCATCACCAACGACAAAACCAAGAGACCCGTGGCCCTAAGAACCA
  AGACTTTGGCAGACCTTTTGGAATCATTTATCGCAGCGCTGTACATCGACAAGGACCTGGAATATGTCCA
  CACTTTCATGAACGTCTGCTTCTTTCCCCGGCTGAAGGAGTTCATTCTGAATCAGGATTGGAACGACCCC
  AAGTCGCAGCTGCAGCAGTGTTGTCTGACCCTGAGGACAGAAGGGAAAGAGCCTGACATCCCCTTATACA
  AGACTCTGCAGACAGTGGGGCCATCCCATGCTAGAACCTACACTGTGGCTGTTTATTTCAAGGGAGAAAG
  GATAGGCTGTGGGAAAGGACCAAGCATTCAGCAGGCGGAGATGGGAGCAGCAATGGATGCACTGGAGAAA
  TATAACTTTCCCCAGATGGCCCATCAGAAGCGGTTCATTGAGCGGAAATACAGACAAGAGTTAAAGGAAA
  TGAGGTGGGAAAGAGAGCATCAGGAGAGAGAGCCGGAGGAGGCTGAAGACATCAAGAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR211952 representing NM_026799
  Red=Cloning site Green=Tags(s)
  
  MQGNTCHRMSYHPGRGCPRGRGGHGARPSAPAFRPQNLRLLHPQQPPAQYQYEPPSAPSSSYSNSQAPSF
  MPPRPDFVPYPPPAAPSAQGPLPPCPVRPPYPNHQMRHPFPVPPCFPPMPPPMPCPNNPPASGAPPGQGT
  FPFMVPPPSMPHPPPPPVMPQQVNYQYPPGYSHSFPPPGFNSYQNNSSSFPPSANSSSTPHFRHLPPYSL
  PKAQNERRSPERLKHYDDHRHRDHSHGRGERHRSLERRERGRSPERRRPESRYRSDYDRGRTPPPRHRSY
  ERSRERDRERHRHREARRSPSLERSYKKEYKRSGRSYALPVAPEPAGCTPELPGEMIKTTESWAPPPENV
  NHRSPSREKKRARWEEEKDRWSDSQGSGKEKNYTSIKEKEAEEVPPEKTEEEEEELLKPVWIRCTHSESY
  YSSDPMDQVGDSTVVGTSRLRDLYDKFEEELGNRQEKAKAARPPWEPPKTKLDEDLESSSESECETDDDS
  TCSSSSDSEVFDVIAEIKRKKAHPDRLHDELWYNDPGQMNDGPLCKCSAKARRTGIRHSIYPGEEAIKPC
  RPMTNNAGRLFHYRITVSPPTNFLTDRPTVIEYDDHEYIFEGFSMFAHAPLTNIPLCKVIRFNIDYTIHF
  IEEMMPENFCVKGLELFSLFLFRDILELYDWNLKGPLFEDSPPCCPRFHFMPRFVRFLPDGGKEVLSMHQ
  ILLYLLRCSKALVPEEEIANMLQWEELEWQKYAEECKGMIVTNPGTKPSSVRIDQLDREQFNPEVITFPI
  IVHFGIRPAQLSYAGDPQYQKLWKSYVKLRHLLANSPKVKQTDKQKLAQREEALQKIRQKNTMRREVTVE
  LSSQGFWKTGIRSDVCQHAMMLPVLTHHIRYHQCLMHLDKLIGYTFQDRCLLQLAMTHPSHHLNFGMNPD
  HARNSLSNCGIRQPKYGDRKVHHMHMRKKGINTLINIMSRLGQDDPTPSRINHNERLEFLGDAVVEFLTS
  VHLYYLFPSLEEGGLATYRTAIVQNQHLAMLAKKLELDRFMLYAHGPDLCRESDLRHAMANCFEALIGAV
  YLEGSLEEAKQLFGRLLFNDPDLREVWLNYPLHPLQLQEPNTDRQLIETSPVLQKLTEFEEAIGVIFTHV
  RLLARAFTLRTVGFNHLTLGHNQRMEFLGDSIMQLVATEYLFIHFPDHHEGHLTLLRSSLVNNRTQAKVA
  EELGMQEYAITNDKTKRPVALRTKTLADLLESFIAALYIDKDLEYVHTFMNVCFFPRLKEFILNQDWNDP
  KSQLQQCCLTLRTEGKEPDIPLYKTLQTVGPSHARTYTVAVYFKGERIGCGKGPSIQQAEMGAAMDALEK
  YNFPQMAHQKRFIERKYRQELKEMRWEREHQEREPEEAEDIKK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_026799
• ORF Size: 4119 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_026799.3, NP_081075.3
• RefSeq Size: 4580 bp
• RefSeq ORF: 4122 bp
• Locus ID: 14000
• UniProt ID: Q5HZJ0
• Cytogenetics: 15 A1
• MW: 159.3 kDa
• Gene Summary: Ribonuclease III double-stranded (ds) RNA-specific endoribonuclease that is involved in the initial step of microRNA (miRNA) biogenesis. Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, DROSHA cleaves the 3' and 5' strands of a stem-loop in pri-miRNAs (processing center 11 bp from the dsRNA-ssRNA junction) to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs. Involved also in pre-rRNA processing. Cleaves double-strand RNA and does not cleave single-strand RNA. Involved in the formation of GW bodies.[UniProtKB/Swiss-Prot Function]