Ctnna1 (NM_009818) Mouse Tagged ORF Clone

CAT#: MR211134

Ctnna1 (Myc-DDK-tagged) - Mouse catenin (cadherin associated protein), alpha 1 (Ctnna1)

Product Images

Circular map for MR211134

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ctnna1
• Synonyms: 2010010M04Rik; AA517462; AI988031; Catna1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR211134 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACTGCCGTCCACGCAGGCAACATAAACTTCAAATGGGACCCCAAAAGTCTGGAGATTAGGACTCTGG
  CTGTTGAAAGACTGTTGGAGCCTCTTGTTACACAGGTTACAACCCTGGTAACCACCAATAGTAAAGGGCC
  GTCTAATAAAAAGAGAGGCCGTTCTAAGAAGGCCCATGTTTTGGCTGCATCTGTTGAACAAGCAACTGAG
  AATTTCTTGGAAAAGGGGGATAAAATTGCAAAAGAGAGCCAGTTTCTCAAGGAGGAGCTTGTGGTTGCTG
  TAGAAGATGTTCGAAAACAAGGAGATTTGATGAAGAGCGCTGCTGGGGAGTTTGCAGATGATCCATGCTC
  TTCTGTGAAGCGAGGCAACATGGTCCGGGCAGCTCGAGCTTTGCTCTCTGCCGTCACCCGGCTGCTGATT
  CTGGCTGACATGGCAGATGTCTACAAATTACTTGTTCAGCTGAAAGTTGTGGAAGATGGTATATTGAAAC
  TGAGGAATGCTGGCAATGAACAAGACTTAGGGATACAGTACAAAGCTCTGAAACCAGAAGTAGATAAGCT
  GAACATCATGGCAGCAAAAAGACAACAGGAACTAAAAGATGTGGGCAATCGTGATCAGATGGCTGCAGCT
  AGAGGAATCCTGCAGAAAAATGTTCCAATCCTCTATACTGCATCCCAGGCATGTCTACAACACCCTGATG
  TTGCAGCCTATAAGGCCAACCGGGACTTGATATACAAGCAGCTGCAGCAGGCCGTCACAGGAATTTCTAA
  TGCAGCCCAGGCTACTGCATCAGATGATGCTGCCCAGCACCAGGGTGGCAGTGGCGGAGAGCTGGCATAC
  GCTCTCAACAACTTTGATAAACAAATCATTGTGGACCCCTTGAGCTTCAGCGAGGAGCGCTTTAGGCCGT
  CCCTGGAGGAGCGCCTGGAAAGCATTATTAGTGGGGCTGCCCTGATGGCTGACTCATCCTGCACACGTGA
  TGACCGTCGGGAGCGCATTGTGGCAGAGTGTAATGCTGTCCGCCAGGCTCTACAGGACCTGCTCTCAGAA
  TACATGGGCAACGCTGGACGTAAAGAAAGAAGTGATGCACTCAACTCTGCAATAGATAAAATGACCAAGA
  AGACCAGGGACTTGCGTAGACAGCTTCGCAAAGCTGTCATGGACCACGTATCAGATTCTTTCCTGGAGAC
  CAATGTCCCTCTATTAGTACTGATTGAAGCTGCAAAGAATGGAAATGAGAAAGAAGTTAAGGAATATGCC
  CAAGTTTTTCGTGAACATGCCAACAAACTGATTGAGGTTGCCAACCTGGCCTGTTCAATCTCCAACAATG
  AAGAAGGCGTGAAGCTTGTCCGAATGTCTGCAAGCCAGTTAGAAGCGCTGTGTCCTCAGGTTATCAATGC
  TGCATTGGCGTTGGCAGCAAAGCCGCAGAGTAAACTGGCCCAAGAGAACATGGATCTTTTTAAAGAGCAA
  TGGGAAAAGCAAGTCCGTGTTCTCACAGATGCTGTTGATGACATTACTTCCATCGATGACTTCTTGGCTG
  TCTCAGAGAACCACATTTTAGAAGATGTGAACAAGTGTGTTATTGCTCTCCAAGAGAAAGACGTGGATGG
  GCTGGATCGCACTGCCGGTGCCATCCGAGGCCGGGCAGCCCGAGTCATTCATGTAGTCACCTCAGAGATG
  GACAACTACGAGCCAGGAGTTTACACAGAGAAGGTTCTGGAAGCCACCAAGCTCCTCTCCAACACAGTCA
  TGCCGCGCTTCACTGAGCAGGTGGAAGCAGCTGTGGAAGCCCTCAGCTCGGACCCGGCCCAGCCCATGGA
  TGAGAATGAGTTCATCGACGCCTCCCGTCTGGTGTACGACGGCATCCGGGACATCCGGAAAGCAGTGCTG
  ATGATCAGGACCCCCGAGGAGTTGGACGACTCTGACTTCGAGACTGAAGACTTTGATGTCAGAAGCAGGA
  CCAGTGTCCAGACAGAAGATGACCAGCTGATAGCTGGCCAGAGCGCCCGGGCGATCATGGCTCAGCTTCC
  TCAGGAGCAAAAAGCAAAGATTGCAGAACAGGTGGCCAGTTTCCAGGAAGAGAAGAGCAAGCTGGATGCT
  GAAGTGTCCAAGTGGGATGACAGTGGCAATGACATCACTGTGCTGGCCAAGCAGATGTGCATGATCATGA
  TGGAGATGACCGACTTCACCCGAGGCAAAGGTCCACTCAAAAACACCTCTGACGTGATCAGTGCTGCCAA
  GAAAATTGCGGAGGCGGGATCCAGGATGGACAAGCTCGGCCGGACCATCGCAGACCATTGCCCAGACTCG
  GTATGCAAGCAGGACCTGCTGGCCTACCTTCAGCGCATCGCTCTCTACTGCCACCAGCTCAACATCTGCA
  GCAAAGTCAAGGCTGAAGTACAGAACCTTGGTGGAGAGCTGGTCGTCTCTGGGGTGGACAGCGCCATGTC
  CCTGATCCAGGCGGCCAAGAACTTGATGAATGCTGTTGTGCAGACAGTGAAGGCGTCCTACGTGGCTTCC
  ACCAAATACCAGAAGTCACAGGGGATGGCTTCCTTGAACCTTCCTGCTGTGTCATGGAAGATGAAGGCCC
  CTGAGAAGAAGCCGTTGGTGAAGAGAGAGAAGCAGGATGAGACGCAGACCAAGATTAAACGAGCTTCTCA
  GAAGAAACACGTGAACCCAGTGCAGGCCCTGAGCGAGTTCAAAGCCATGGACAGCATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR211134 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MTAVHAGNINFKWDPKSLEIRTLAVERLLEPLVTQVTTLVTTNSKGPSNKKRGRSKKAHVLAASVEQATE
  NFLEKGDKIAKESQFLKEELVVAVEDVRKQGDLMKSAAGEFADDPCSSVKRGNMVRAARALLSAVTRLLI
  LADMADVYKLLVQLKVVEDGILKLRNAGNEQDLGIQYKALKPEVDKLNIMAAKRQQELKDVGNRDQMAAA
  RGILQKNVPILYTASQACLQHPDVAAYKANRDLIYKQLQQAVTGISNAAQATASDDAAQHQGGSGGELAY
  ALNNFDKQIIVDPLSFSEERFRPSLEERLESIISGAALMADSSCTRDDRRERIVAECNAVRQALQDLLSE
  YMGNAGRKERSDALNSAIDKMTKKTRDLRRQLRKAVMDHVSDSFLETNVPLLVLIEAAKNGNEKEVKEYA
  QVFREHANKLIEVANLACSISNNEEGVKLVRMSASQLEALCPQVINAALALAAKPQSKLAQENMDLFKEQ
  WEKQVRVLTDAVDDITSIDDFLAVSENHILEDVNKCVIALQEKDVDGLDRTAGAIRGRAARVIHVVTSEM
  DNYEPGVYTEKVLEATKLLSNTVMPRFTEQVEAAVEALSSDPAQPMDENEFIDASRLVYDGIRDIRKAVL
  MIRTPEELDDSDFETEDFDVRSRTSVQTEDDQLIAGQSARAIMAQLPQEQKAKIAEQVASFQEEKSKLDA
  EVSKWDDSGNDITVLAKQMCMIMMEMTDFTRGKGPLKNTSDVISAAKKIAEAGSRMDKLGRTIADHCPDS
  VCKQDLLAYLQRIALYCHQLNICSKVKAEVQNLGGELVVSGVDSAMSLIQAAKNLMNAVVQTVKASYVAS
  TKYQKSQGMASLNLPAVSWKMKAPEKKPLVKREKQDETQTKIKRASQKKHVNPVQALSEFKAMDSI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009818
• ORF Size: 2721 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009818.1, NP_033948.1
• RefSeq Size: 3713 bp
• RefSeq ORF: 2721 bp
• Locus ID: 12385
• UniProt ID: P26231
• Cytogenetics: 18 18.89 cM
• MW: 100.1 kDa
• Gene Summary: Associates with the cytoplasmic domain of a variety of cadherins. The association of catenins to cadherins produces a complex which is linked to the actin filament network, and which seems to be of primary importance for cadherins cell-adhesion properties. Can associate with both E- and N-cadherins. Originally believed to be a stable component of E-cadherin/catenin adhesion complexes and to mediate the linkage of cadherins to the actin cytoskeleton at adherens junctions. In contrast, cortical actin was found to be much more dynamic than E-cadherin/catenin complexes and CTNNA1 was shown not to bind to F-actin when assembled in the complex suggesting a different linkage between actin and adherens junctions components. The homodimeric form may regulate actin filament assembly and inhibit actin branching by competing with the Arp2/3 complex for binding to actin filaments. May play a crucial role in cell differentiation.[UniProtKB/Swiss-Prot Function]