Csde1 (NM_144901) Mouse Tagged ORF Clone

CAT#: MR210719

Csde1 (Myc-DDK-tagged) - Mouse cold shock domain containing E1, RNA binding (Csde1), transcript variant 1

Product Images

Circular map for MR210719

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Csde1
• Synonyms: AA960392; BC016898; D3Jfr1; mKIAA0885; unr
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR210719 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCTTTGATCCAAACCTTCTCCACAACAATGGACACAATGGGTACCCCAATGGTACTTCAGCAGCAC
  TTCGTGAAACTGGGGTTATTGAAAAACTCTTGACCTCTTACGGATTCATTCAGTGTTCAGAACGGCAAGC
  TAGACTTTTCTTCCACTGTTCACAATATAATGGCAACCTCCAAGACTTAAAAGTAGGAGATGATGTTGAA
  TTTGAAGTATCATCTGACCGGAGGACTGGGAAACCTATTGCTATTAAATTGGTGAAGATAAAACCAGAAA
  TACATCCTGAAGAACGAATGAACGGACAAGTTGTGTGCGCTGTTCCTCACAACTTAGAGAGTAAATCTCC
  AGCTGCCCCGGGTCAGAGTCCAACAGGGAGTGTATGCTACGAACGTAATGGGGAAGTATTTTATCTGACT
  TACACCTCTGAAGATGTGGAAGGGAATGTTCAGCTGGAAACTGGAGATAAAATTAACTTTGTAATTGATA
  ACAATAAACACACTGGTGCTGTAAGTGCTCGTAATATTATGCTGTTGAAAAAGAAGCAGGCTCGCTGTCA
  AGGAGTAGTTTGTGCCATGAAGGAGGCGTTTGGCTTTATCGAAAGAGGTGATGTTGTAAAAGAGATATTC
  TTTCACTATAGTGAATTTAAAGGTGACCTAGAAACCCTACAGCCTGGAGATGACGTGGAATTCACAATCA
  AGGACAGAAATGGTAAAGAAGTTGCAACAGATGTCAGACTATTGCCTCAAGGAACAGTCATTTTTGAAGA
  TATCAGCATTGAACATTTTGAAGGAACTGTAACCAAAGTTATCCCAAAAGTACCCAGTAAAAACCAGAAT
  GACCCATTGCCAGGACGAATCAAAGTTGACTTTGTGATTCCTAAAGAACTTCCCTTTGGAGACAAAGACA
  CAAAATCCAAAGTGACCCTGCTGGAAGGTGACCATGTTAGGTTTAATATTTCAACAGACCGACGTGACAA
  ATTGGAACGAGCAACCAACATAGAGGTTCTATCAAATACATTTCAGTTCACTAATGAAGCCAGAGAGATG
  GGTGTGATTGCTGCCATGAGAGATGGTTTTGGTTTCATCAAGTGTGTGGATCGTGATGCTCGTATGTTCT
  TCCACTTCAGTGAGATTCTAGATGGGAACCAGCTCCACATTGCAGATGAAGTAGAGTTTACTGTGGTTCC
  TGATATGCTCTCTGCCCAAAGAAATCATGCTATTAGGATTAAAAAACTTCCCAAGGGCACGGTTTCATTC
  CATTCCCATTCAGATCATCGTTTTCTGGGCACCGTAGAAAAAGAAGCCACTTTTTCCAATCCTAAAACTA
  CAAGCCCAAATAAAGGCAAAGACAAGGAGGCAGAAGATGGCATTATAGCTTATGACGACTGTGGGGTGAA
  ACTGACGATTGCTTTTCAAGCCAAGGATGTGGAAGGATCTACTTCTCCTCAAATAGGAGATAAGGTTGAA
  TTTAGTATTAGTGACAAACAGAGGCCTGGACAGCAGATTGCAACTTGCGTGAGACTTTTAGGTCGTAATT
  CTAACTCCAAACGTCTCTTGGGTTATGTGGCAACTCTGAAAGATAATTTTGGATTTATAGAAACAGCTAA
  TCATGATAAGGAAATATTTTTCCACTATAGTGAGTTCTCTGGTGATGTTGATAGCCTGGAACTGGGAGAC
  ATGGTTGAATACAGCTTGTCCAAAGGCAAAGGCAATAAAGTCAGTGCTGAGAAAGTAAACAAAGCCCACT
  CAGTGAATGGCATTACTGAGGAAGCTAATCCCACCATCTACTCTGGTAAAGTCATTCGCCCTCTGAGAGG
  TGTTGATCCAACACAGATTGAGTACCAAGGAATGATTGAGATTGTGGAGGAGGGGGATATGAAAGGTGAA
  GTGTATCCTTTTGGCATAGTTGGGATGGCCAACAAAGGGGATTGCCTACAGAAAGGGGAGAGTGTCAAGT
  TCCAGTTGTGTGTACTTGGCCAAAATGCACAAACTATGGCCTACAACATCACACCCCTTCGTAGGGCTAC
  TGTGGAGTGTGTGAAAGATCAGTTTGGCTTTATTAACTATGAAGTAGGAGATAGCAAGAAGCTCTTTTTC
  CATGTGAAAGAAGTTCAGGATGGCGTTGAGCTACAGGCAGGAGATGAGGTGGAATTCTCAGTGATCCTTA
  ATCAGCGCACTGGGAAGTGCAGTGCCTGTAATGTCTGGCGAGTCTGTGAAGGCCCCAAAGCTGTTGCAGC
  TCCTCGACCTGACCGGTTGGTCAATCGCCTAAAGAACATCACCCTGGATGATGCCAGTGCTCCACGTCTA
  ATGGTTCTTCGTCAGCCAAGGGGACCAGATAACTCAATGGGATTTGGTGCAGAAAGAAAGATCCGTCAAG
  CTGGTGTCATTGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR210719 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSFDPNLLHNNGHNGYPNGTSAALRETGVIEKLLTSYGFIQCSERQARLFFHCSQYNGNLQDLKVGDDVE
  FEVSSDRRTGKPIAIKLVKIKPEIHPEERMNGQVVCAVPHNLESKSPAAPGQSPTGSVCYERNGEVFYLT
  YTSEDVEGNVQLETGDKINFVIDNNKHTGAVSARNIMLLKKKQARCQGVVCAMKEAFGFIERGDVVKEIF
  FHYSEFKGDLETLQPGDDVEFTIKDRNGKEVATDVRLLPQGTVIFEDISIEHFEGTVTKVIPKVPSKNQN
  DPLPGRIKVDFVIPKELPFGDKDTKSKVTLLEGDHVRFNISTDRRDKLERATNIEVLSNTFQFTNEAREM
  GVIAAMRDGFGFIKCVDRDARMFFHFSEILDGNQLHIADEVEFTVVPDMLSAQRNHAIRIKKLPKGTVSF
  HSHSDHRFLGTVEKEATFSNPKTTSPNKGKDKEAEDGIIAYDDCGVKLTIAFQAKDVEGSTSPQIGDKVE
  FSISDKQRPGQQIATCVRLLGRNSNSKRLLGYVATLKDNFGFIETANHDKEIFFHYSEFSGDVDSLELGD
  MVEYSLSKGKGNKVSAEKVNKAHSVNGITEEANPTIYSGKVIRPLRGVDPTQIEYQGMIEIVEEGDMKGE
  VYPFGIVGMANKGDCLQKGESVKFQLCVLGQNAQTMAYNITPLRRATVECVKDQFGFINYEVGDSKKLFF
  HVKEVQDGVELQAGDEVEFSVILNQRTGKCSACNVWRVCEGPKAVAAPRPDRLVNRLKNITLDDASAPRL
  MVLRQPRGPDNSMGFGAERKIRQAGVID
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_144901
• ORF Size: 2397 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_144901.4
• RefSeq Size: 4201 bp
• RefSeq ORF: 2397 bp
• Locus ID: 229663
• UniProt ID: Q91W50
• Cytogenetics: 3 45.25 cM
• MW: 88.8 kDa
• Gene Summary: RNA-binding protein. May be involved in translationally coupled mRNA turnover. Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain (By similarity).[UniProtKB/Swiss-Prot Function]