Rasgrp1 (NM_011246) Mouse Tagged Lenti ORF Clone
CAT#: MR210704L3
- LentiORF®
Lenti ORF clone of Rasgrp1 (Myc-DDK-tagged) - Mouse RAS guanyl releasing protein 1 (Rasgrp1)
"NM_011246" in other vectors (4)
Interest in protein/lysate? Submit request here!
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Rasgrp1 |
Synonyms | Rasgrp |
Vector | pLenti-C-Myc-DDK-P2A-Puro |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | Puromycin |
Sequence Data |
The ORF insert of this clone is exactly the same as(MR210704).
|
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_011246 |
ORF Size | 2385 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_011246.2 |
RefSeq Size | 5155 bp |
RefSeq ORF | 2388 bp |
Locus ID | 19419 |
UniProt ID | Q9Z1S3 |
Cytogenetics | 2 59.19 cM |
Gene Summary | Functions as a calcium- and diacylglycerol (DAG)-regulated nucleotide exchange factor specifically activating Ras through the exchange of bound GDP for GTP. Activates the Erk/MAP kinase cascade. Regulates T-cell/B-cell development, homeostasis and differentiation by coupling T-lymphocyte/B-lymphocyte antigen receptors to Ras. Regulates NK cell cytotoxicity and ITAM-dependent cytokine production by activation of Ras-mediated ERK and JNK pathways (By similarity). Functions in mast cell degranulation and cytokine secretion, regulating FcERI-evoked allergic responses (PubMed:17190838). May also function in differentiation of other cell types. Proto-oncogene, which promotes T-cell lymphomagenesis when its expression is deregulated (PubMed:15829980, PubMed:17210708).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC221698 | Rasgrp1 (untagged) - Mouse RAS guanyl releasing protein 1 (Rasgrp1), (10ug) |
USD 802.00 |
|
MG210704 | Rasgrp1 (tGFP-tagged) - Mouse RAS guanyl releasing protein 1 (Rasgrp1) |
USD 928.00 |
|
MR210704 | Rasgrp1 (Myc-DDK-tagged) - Mouse RAS guanyl releasing protein 1 (Rasgrp1) |
USD 728.00 |
|
MR210704L4 | Lenti ORF clone of Rasgrp1 (mGFP-tagged) - Mouse RAS guanyl releasing protein 1 (Rasgrp1) |
USD 1,028.00 |
{0} Product Review(s)
Be the first one to submit a review