Cfb (NM_001142706) Mouse Tagged ORF Clone

CAT#: MR210521

Cfb (Myc-DDK-tagged) - Mouse complement factor B (Cfb), transcript variant 2

Product Images

Circular map for MR210521

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cfb
• Synonyms: AI195813; AI255840; B; Bf; C2; Fb; H2-Bf
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR210521 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGAGCCCCCAGCTCTGCCTCGTCCTCTTGGTCTTAGGCTTCTCCTCTGGAGGTGTGAGCGCAACTC
  CAGTGCTTGAGGCCCGGCCCCAAGTCTCCTGCTCTCTGGAGGGAGTAGAGATCAAAGGCGGCTCCTTTCA
  ACTTCTCCAAGGCGGTCAGGCCCTGGAGTACCTATGTCCCTCTGGCTTCTACCCATACCCTGTGCAGACT
  CGAACCTGCAGATCCACAGGCTCCTGGAGCGACCTGCAGACCCGAGACCAAAAGATTGTCCAGAAGGCGG
  AATGCAGAGCAATACGCTGCCCACGACCGCAGGACTTTGAAAATGGGGAATTCTGGCCCCGGTCCCCCTT
  CTACAACCTGAGTGACCAGATTTCTTTTCAATGCTATGATGGTTACGTTCTCCGGGGCTCTGCTAATCGC
  ACCTGCCAAGAGAATGGCCGGTGGGATGGGCAAACAGCAATTTGTGATGATGGAGCTGGATACTGTCCCA
  ATCCTGGTATTCCTATTGGGACAAGGAAGGTGGGTAGCCAATACCGCCTTGAAGACATTGTTACTTACCA
  CTGCAGCCGGGGACTTGTCCTGCGTGGCTCCCAGAAGCGAAAGTGTCAAGAAGGTGGCTCATGGAGTGGG
  ACAGAGCCTTCCTGCCAAGATTCCTTCATGTATGACAGCCCTCAAGAAGTGGCCGAAGCATTCCTATCCT
  CCCTGACAGAGACCATCGAAGGAGCCGATGCTGAGGATGGGCACAGCCCAGGGGAACAGCAGAAGAGGAA
  GATTGTCCTAGACCCCTCGGGCTCCATGAATATCTACCTGGTGCTAGATGGTTCCGACAGCATCGGAAGC
  AGCAACTTCACAGGGGCTAAGCGGTGCCTCACCAACTTGATTGAGAAGGTGGCGAGTTACGGGGTGAGGC
  CACGATATGGTCTCCTGACATATGCTACAGTCCCCAAAGTGTTGGTCAGAGTGTCTGATGAGAGGAGTAG
  CGATGCCGACTGGGTCACAGAGAAGCTCAACCAAATCAGTTATGAAGACCACAAGCTGAAGTCAGGGACC
  AACACCAAGAGGGCTCTCCAGGCTGTGTATAGCATGATGAGCTGGGCAGGGGATGCCCCGCCTGAAGGCT
  GGAATAGAACCCGCCATGTCATCATCATTATGACTGATGGCTTGCACAACATGGGTGGAAACCCTGTCAC
  TGTCATTCAGGACATCCGAGCCTTGCTGGACATCGGCAGGGATCCCAAAAATCCCAGGGAGGATTACCTG
  GATGTGTATGTGTTTGGGGTCGGGCCTCTGGTGGACTCCGTGAACATCAATGCCTTAGCTTCCAAAAAGG
  ACAATGAGCATCATGTGTTTAAAGTCAAGGATATGGAAGACCTGGAGAATGTTTTCTACCAAATGATTGA
  TGAAACCAAATCTCTGAGTCTCTGTGGCATGGTGTGGGAGCATAAAAAAGGCAACGATTATCATAAGCAA
  CCATGGCAAGCCAAGATCTCAGTCACTCGCCCTCTGAAAGGACATGAGACCTGTATGGGGGCCGTGGTGT
  CTGAGTACTTCGTGCTGACAGCAGCGCACTGCTTCATGGTGGATGATCAGAAACATTCCATCAAGGTCAG
  CGTGGGGGGTCAGAGGCGGGACCTGGAGATTGAAGAGGTCCTGTTCCACCCCAAATACAATATTAATGGG
  AAAAAGGCAGAAGGGATCCCTGAGTTCTATGATTATGATGTGGCCCTAGTCAAGCTCAAGAACAAGCTCA
  AGTATGGCCAGACTCTCAGGCCCATCTGTCTCCCCTGCACGGAGGGAACCACACGAGCCTTGAGGCTTCC
  TCAGACAGCCACCTGCAAGCAGCACAAGGAACAGTTGCTCCCTGTGAAGGATGTCAAAGCTCTGTTTGTA
  TCTGAGCAAGGGAAGAGCCTGACTCGGAAGGAGGTGTACATCAAGAATGGGGACAAGAAAGCCAGTTGTG
  AGAGAGATGCTACAAAGGCCCAAGGCTATGAGAAGGTCAAAGATGCCTCTGAGGTGGTCACTCCACGGTT
  CCTCTGCACAGGAGGGGTGGATCCCTATGCTGACCCCAACACATGCAAAGGAGATTCCGGGGGCCCTCTC
  ATTGTTCACAAGAGAAGCCGCTTCATTCAAGTTGGTGTGATTAGCTGGGGAGTAGTAGATGTCTGCAGAG
  ACCAGAGGCGGCAACAGCTGGTACCCTCTTATGCCCGGGACTTCCACATCAACCTCTTCCAGGTGCTGCC
  CTGGCTAAAGGACAAGCTCAAAGATGAGGATTTGGGTTTTCTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR210521 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MESPQLCLVLLVLGFSSGGVSATPVLEARPQVSCSLEGVEIKGGSFQLLQGGQALEYLCPSGFYPYPVQT
  RTCRSTGSWSDLQTRDQKIVQKAECRAIRCPRPQDFENGEFWPRSPFYNLSDQISFQCYDGYVLRGSANR
  TCQENGRWDGQTAICDDGAGYCPNPGIPIGTRKVGSQYRLEDIVTYHCSRGLVLRGSQKRKCQEGGSWSG
  TEPSCQDSFMYDSPQEVAEAFLSSLTETIEGADAEDGHSPGEQQKRKIVLDPSGSMNIYLVLDGSDSIGS
  SNFTGAKRCLTNLIEKVASYGVRPRYGLLTYATVPKVLVRVSDERSSDADWVTEKLNQISYEDHKLKSGT
  NTKRALQAVYSMMSWAGDAPPEGWNRTRHVIIIMTDGLHNMGGNPVTVIQDIRALLDIGRDPKNPREDYL
  DVYVFGVGPLVDSVNINALASKKDNEHHVFKVKDMEDLENVFYQMIDETKSLSLCGMVWEHKKGNDYHKQ
  PWQAKISVTRPLKGHETCMGAVVSEYFVLTAAHCFMVDDQKHSIKVSVGGQRRDLEIEEVLFHPKYNING
  KKAEGIPEFYDYDVALVKLKNKLKYGQTLRPICLPCTEGTTRALRLPQTATCKQHKEQLLPVKDVKALFV
  SEQGKSLTRKEVYIKNGDKKASCERDATKAQGYEKVKDASEVVTPRFLCTGGVDPYADPNTCKGDSGGPL
  IVHKRSRFIQVGVISWGVVDVCRDQRRQQLVPSYARDFHINLFQVLPWLKDKLKDEDLGFL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001142706
• ORF Size: 2286 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq Size: 2763 bp
• RefSeq ORF: 2142 bp
• Locus ID: 14962
• Cytogenetics: 17 18.41 cM
• MW: 85 kDa