Acap1 (NM_153788) Mouse Tagged ORF Clone

CAT#: MR210394

Acap1 (Myc-DDK-tagged) - Mouse ArfGAP with coiled-coil, ankyrin repeat and PH domains 1 (Acap1)

Product Images

Circular map for MR210394

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Acap1
• Synonyms: Centb1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR210394 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACGGTCAAGCTAGATTTTGAAGAATGTCTCAAGGACTCACCCCGCTTCCGAGCCTCGATTGAACTGG
  TGGAAACTGAAGTGTCAGAATTGGAGACCCGCCTGGAAAAGCTGCTAAAGCTGGGTTCCTGCCTCCTGGA
  GAGCGGACAGCATTACCTTGCTGCCGGTCGTGCCTTCGTTGTTGGCATCTGTGACCTGGCTCGCCTGGGT
  CCACCAGAGCCCATGATGGCGGAGTGTCTGGAAAAATTCACCGTGAGTCTGAACCACAAACTGGACAGCC
  ATGCTGAGCTTCTTGATGCTACTCAACATACACTGCAGCAGCAAATCCAGACTCTGGTCAAGGAAGGTCT
  CCGGGGCTTCCGAGAGGCTCGCAGGGATTTCTGGCGAGGGGCTGAGAACCTGGAGGCTGCTCTTACCCAC
  AATGCAGAGGTCCCCAGGCGCCGTGTCCAAGAAGCAGAGGAGGCTGGAACTGCTTTGAGGACCGCTCGAG
  CTGGGTACCGGAGTCGGGCATTAGATTATGCCCTGCAGGTCAATGTGATTGAGGATAAGAGGAAGTTCGA
  CATCATGGAATTCGTACTGCGCTTGGTTGAGGCCCAGGCCACTTATTTCCAGCAGGGCCATGAAGAGCTA
  AACAGGCTGGCTCAGTATCGGAAGGAATTGGGTACCCAGTTGCACAACCTGGTCTTGAACTCAGCTCGAC
  AGAAGAGAGACATGGAACAGAGGCATGTGTTACTGAAGCAGAAGGAGCTGGGTGGCGAGGAGCCAGAGCC
  AAGCCTAAAGGAGGGGCCTAGTGGCTTGGTAATGGAAGGACATCTTTTCAAACGTGCCAGCAATGCATTT
  AAGACCTGGAGCAGACGCTGGTTCACCATTCAGAACAACCAGCTGGTTTACCAGAAGAAATATAAGGACC
  CTGTAACCGTGGTGGTAGACGACCTTCGTCTCTGTACAGTGAAGCTCTGTCCAGACTCAGAAAGGCGTTT
  CTGTTTCGAAGTAGTGTCCACCAGCAAGTCCTGTTTCCTCCAGGCTGACTCTGAGCGTCTCCTGCAGCTG
  TGGGTCAGTGCTGTCCAGAGCAGCATTGCCTCAGCCTTCAGCCAGGCTCACCTGGAGAACAGCCCTCGGG
  GGCCAGGCCAGGTCTCAGGATACCATGCCCCGGGCTCTGCTGCAACTCTGGCCTGTGGCGGGGCAGCCAG
  AGGAAGGGAGTCTGGAGGAGTTGGTCAGGTAGCGGCCCAGGTACAAAGCGTGGATGGAAATGCGCAATGC
  TGCGACTGCAGGGAGCCAGCCCCAGAGTGGGCCAGCATCAACCTTGGCGTCACTCTCTGCATTCAGTGTT
  CTGGCATCCACAGGAGCCTCGGAGTTCATTTCTCCAAAGTCCGCTCTCTGACCCTTGACTCCTGGGAACC
  AGAACTGGTGAAGCTTATGTGCGAGCTGGGGAATGTCATCATCAACCAGATCTATGAGGCCCGGGTGGAG
  GCCATGGCGGTGAAGAAGCCAGGGCCCAGCTGCTCCCGGCAGGAAAAGGAAGCCTGGATTCATGCCAAGT
  ATGTGGAGAAAAAGTTCCTGACCAAACTTCCTGAAATCCGTGGGCGAAGAGGTGGCAGGGGACCCCCAAG
  AGGACATCCTCCTGTGCCCCCAAAGCCTCCCATCAGGCCACACTCGGGCATTGTCAGATCCAAATCAGAG
  TGCCCGTCTGATGACATGGGAAGCCTGCACCCCGGGGCCCTGCTGTTTCAAGCTGCTGGACATCCCCCAT
  CTCTTCCTACCATGGCTGATGCCCTGGCCCATGGAGCTGATGTCAACTGGGTCAATGTGGGCCAGGGGAA
  TGCCACACCTCTGATCCGGGCCACAGCTGCTAACTCTCTTCTGGCCTGCGAGTTTCTCCTCCAAAATGGG
  GCGAATGTGAACCAAGCAGACAGTGCCGGCCGGGGTCCTCTCCACCATGCCACCATTCTTGGTCACACAG
  GGCTCGCTTGCCTGTTCCTGAAACGGGGTGCTGATCTGGGGGCTCGAGACACAGAAGGCAGGGACCCTCT
  GACTATCGCAATGGAAACCACTAATGCTGACATCGTAACCCTGCTACGATTGGCAAAGATGAGGGAGGCT
  GAAGCGGCCCAGGGCCAGGCAGGAGACGAGACGTATCTCGACATATTCCGAGACTTCTCCCTCATGGCGT
  CGGACGACCCAGAGAAACTGAGCCGTCGCAGTCACGACCTCCATACTCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR210394 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MTVKLDFEECLKDSPRFRASIELVETEVSELETRLEKLLKLGSCLLESGQHYLAAGRAFVVGICDLARLG
  PPEPMMAECLEKFTVSLNHKLDSHAELLDATQHTLQQQIQTLVKEGLRGFREARRDFWRGAENLEAALTH
  NAEVPRRRVQEAEEAGTALRTARAGYRSRALDYALQVNVIEDKRKFDIMEFVLRLVEAQATYFQQGHEEL
  NRLAQYRKELGTQLHNLVLNSARQKRDMEQRHVLLKQKELGGEEPEPSLKEGPSGLVMEGHLFKRASNAF
  KTWSRRWFTIQNNQLVYQKKYKDPVTVVVDDLRLCTVKLCPDSERRFCFEVVSTSKSCFLQADSERLLQL
  WVSAVQSSIASAFSQAHLENSPRGPGQVSGYHAPGSAATLACGGAARGRESGGVGQVAAQVQSVDGNAQC
  CDCREPAPEWASINLGVTLCIQCSGIHRSLGVHFSKVRSLTLDSWEPELVKLMCELGNVIINQIYEARVE
  AMAVKKPGPSCSRQEKEAWIHAKYVEKKFLTKLPEIRGRRGGRGPPRGHPPVPPKPPIRPHSGIVRSKSE
  CPSDDMGSLHPGALLFQAAGHPPSLPTMADALAHGADVNWVNVGQGNATPLIRATAANSLLACEFLLQNG
  ANVNQADSAGRGPLHHATILGHTGLACLFLKRGADLGARDTEGRDPLTIAMETTNADIVTLLRLAKMREA
  EAAQGQAGDETYLDIFRDFSLMASDDPEKLSRRSHDLHTL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_153788
• ORF Size: 2223 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_153788.2
• RefSeq Size: 2482 bp
• RefSeq ORF: 2223 bp
• Locus ID: 216859
• UniProt ID: Q8K2H4
• Cytogenetics: 11 B3
• MW: 81.7 kDa
• Gene Summary: GTPase-activating protein (GAP) for ADP ribosylation factor 6 (ARF6) required for clathrin-dependent export of proteins from recycling endosomes to trans-Golgi network and cell surface. Required for regulated export of ITGB1 from recycling endosomes to the cell surface and ITGB1-dependent cell migration (By similarity).[UniProtKB/Swiss-Prot Function]