Tap2 (NM_011530) Mouse Tagged ORF Clone

CAT#: MR210132

Tap2 (Myc-DDK-tagged) - Mouse transporter 2, ATP-binding cassette, sub-family B (MDR/TAP) (Tap2)

Product Images

Circular map for MR210132

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Tap2
• Synonyms: ABC18; Abcb; Abcb3; AI462429; APT2; Ham; Ham-; Ham-2; Ham2; jas; MTP; MTP2; PS; PSF2; RING11; Tap; Tap-2; Y1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR210132 representing NM_011530
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGCTGTCCTACCTGAGGCCCTGGGTCTCTCTGCTGCTGGCGGACATGGCTTTACTTGGGTTGCTAC
  AAGGATCTCTGGGAAATCTGCTTCCCCAGGGGCTGCCAGGACTCTGGATAGAGGGCACCCTGCGACTTGG
  AGTGCTGTGGGGACTGCTAAAAGTGGGAGAGCTGCTGGGACTTGTGGGGACCCTTCTGCCCTTGCTCTGC
  CTTGCCACTCCCCTGTTTTTCTCGCTAAGAGCTCTGGTGGGAGGCACCGCGAGCACCTCAGTAGTCCGAG
  TGGCTTCTGCCTCTTGGGGCTGGCTGCTGGCTGGCTATGGGGCTGTTGCGCTGAGCTGGGCCGTGTGGGC
  TGTGCTGAGCCCGGCTGGAGTCCAGGAGAAGGAACCAGGCCAGGAGAACAGAACACTGATGAAGCGGTTG
  CTGAAGCTGTCCAGGCCGGACCTGCCTTTCCTCATAGCTGCCTTCTTCTTCCTTGTGGTGGCTGTGTGGG
  GGGAGACATTAATCCCTCGCTATTCGGGTCGTGTAATTGACATCCTGGGAGGTGATTTCGACCCCGACGC
  CTTTGCAAGCGCCATCTTTTTCATGTGCCTGTTCTCTGTTGGGAGCTCCTTCTCTGCAGGCTGTAGAGGA
  GGCTCCTTCCTCTTCACCATGTCCAGGATCAACCTGCGGATACGAGAGCAGCTTTTCTCATCTTTGTTGC
  GCCAAGACCTTGGATTCTTCCAGGAGACCAAGACAGGGGAGCTGAACTCGAGGCTGAGCTCTGACACCTC
  TCTGATGAGCCGCTGGCTCCCTTTCAATGCCAATATCCTGCTGCGGAGCCTGGTGAAGGTGGTGGGGCTC
  TACTTCTTCATGCTCCAGGTATCGCCCCGACTCACCTTCCTCTCCCTGCTGGACCTGCCCCTCACGATAG
  CAGCTGAGAAGGTGTACAACCCCCGCCATCAGGCGGTGCTAAAGGAGATCCAGGATGCAGTGGCCAAGGC
  GGGGCAGGTGGTGCGCGAGGCGGTAGGAGGGCTGCAGACTGTGCGAAGCTTTGGGGCCGAGGAGCAGGAA
  GTCAGCCACTACAAGGAGGCCCTGGAGCGATGTAGACAGCTGTGGTGGCGCCGAGACCTGGAAAAAGACG
  TGTATCTAGTCATACGGAGGGTGATGGCCTTGGGCATGCAGGTGCTGATTCTGAACTGCGGCGTGCAGCA
  GATTCTGGCTGGAGAGGTCACCCGGGGTGGCCTGCTCTCCTTCCTGCTGTACCAGGAGGAAGTGGGACAA
  TATGTCCGGAACCTGGTTTACATGTACGGGGATATGCTGAGCAACGTGGGCGCTGCTGAAAAGGTGTTTT
  CCTACCTGGACCGAAAGCCGAATCTGCCCCAGCCTGGGATCCTGGCCCCTCCCTGGCTGGAGGGGCGCGT
  GGAATTCCAAGACGTCTCCTTTTCGTATCCCAGGCGCCCCGAGAAGCCTGTGCTCCAGGGTCTGACGTTC
  ACCCTGCATCCTGGAACGGTGACAGCGTTGGTGGGACCCAATGGATCAGGGAAGAGCACCGTGGCCGCCC
  TGCTGCAGAACCTGTACCAGCCCACTGGGGGCCAGCTGCTGCTGGATGGCGAGCCCCTGACCGAGTATGA
  TCACCACTACCTGCACCGCCAGGTGGTTCTGGTGGGGCAGGAGCCTGTGCTGTTCTCGGGTTCTGTCAAG
  GACAATATTGCCTATGGCCTGAGGGACTGTGAGGACGCTCAAGTGATGGCAGCTGCCCAGGCGGCCTGTG
  CAGACGACTTCATAGGGGAAATGACTAATGGAATAAACACAGAAATCGGGGAAAAAGGGGGCCAGTTAGC
  TGTGGGACAGAAGCAACGTCTGGCCATTGCCCGGGCCCTTGTGCGGAACCCACGGGTCCTCATCCTGGAT
  GAGGCTACCAGCGCCCTGGACGCCCAGTGTGAACAGGCCCTACAGAACTGGAGATCGCAGGGGGACAGGA
  CGATGCTGGTGATTGCCCACAGGCTGCACACGGTTCAGAATGCTGACCAAGTTCTGGTGCTCAAGCAGGG
  ACGTCTGGTGGAGCATGACCAGCTCAGGGACGGCCAGGATGTCTACGCCCACCTGGTACAGCAGCGGCTG
  GAGGCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR210132 representing NM_011530
  Red=Cloning site Green=Tags(s)
  
  MALSYLRPWVSLLLADMALLGLLQGSLGNLLPQGLPGLWIEGTLRLGVLWGLLKVGELLGLVGTLLPLLC
  LATPLFFSLRALVGGTASTSVVRVASASWGWLLAGYGAVALSWAVWAVLSPAGVQEKEPGQENRTLMKRL
  LKLSRPDLPFLIAAFFFLVVAVWGETLIPRYSGRVIDILGGDFDPDAFASAIFFMCLFSVGSSFSAGCRG
  GSFLFTMSRINLRIREQLFSSLLRQDLGFFQETKTGELNSRLSSDTSLMSRWLPFNANILLRSLVKVVGL
  YFFMLQVSPRLTFLSLLDLPLTIAAEKVYNPRHQAVLKEIQDAVAKAGQVVREAVGGLQTVRSFGAEEQE
  VSHYKEALERCRQLWWRRDLEKDVYLVIRRVMALGMQVLILNCGVQQILAGEVTRGGLLSFLLYQEEVGQ
  YVRNLVYMYGDMLSNVGAAEKVFSYLDRKPNLPQPGILAPPWLEGRVEFQDVSFSYPRRPEKPVLQGLTF
  TLHPGTVTALVGPNGSGKSTVAALLQNLYQPTGGQLLLDGEPLTEYDHHYLHRQVVLVGQEPVLFSGSVK
  DNIAYGLRDCEDAQVMAAAQAACADDFIGEMTNGINTEIGEKGGQLAVGQKQRLAIARALVRNPRVLILD
  EATSALDAQCEQALQNWRSQGDRTMLVIAHRLHTVQNADQVLVLKQGRLVEHDQLRDGQDVYAHLVQQRL
  EA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_011530
• ORF Size: 2106 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_011530.3, NP_035660.3
• RefSeq Size: 2490 bp
• RefSeq ORF: 2109 bp
• Locus ID: 21355
• UniProt ID: P36371
• Cytogenetics: 17 17.98 cM
• MW: 77.4 kDa
• Gene Summary: The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. The protein encoded by this gene is involved in antigen presentation. This protein forms a heterodimer with Tap1 in order to transport peptides from the cytoplasm to the endoplasmic reticulum. Mutations in the human gene may be associated with ankylosing spondylitis, insulin-dependent diabetes mellitus, and celiac disease. [provided by RefSeq, Jul 2008]