Arid3a (NM_007880) Mouse Tagged ORF Clone

CAT#: MR209313

Arid3a (Myc-DDK-tagged) - Mouse AT rich interactive domain 3A (BRIGHT-like) (Arid3a)

Product Images

Circular map for MR209313

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Arid3a
• Synonyms: Bright; Dri1; Dril1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR209313 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGCTGCAGGCTGTGATGGAGACTCTCATTCAGAGGCAGCAGCGTGCCCGGCAGGAACTGGAGGCTC
  GGCAGGCTCCACCACCCCCACCACCTGAGCCCACTGGAGTCCGGGCACGGACCACCATGACAGATGAGGA
  CAGGGAGCCTGAGAATGCCCGGATGCATAGGACTCAGATGGCCGCACTGGCTGCCATGCGAGCTGCTGCT
  GCCGGCTTGGGACATCCGTCCTCTCCCGGGGGCTCTGAGGATGGGCCTCCCATCTCTGGGGATGAAGACA
  CAGCCCGGGAAGGGACTCTGAGTTCACCTGCCCTGCATGGAAGTGTCCTGGAGGGAGCAGGACACGCTGA
  GGGAGATAGGCATTTGATGGACGTGGGCTCTGATGATGATGACACGAAGTCCAAGTGGGAAGAGCAAGAG
  CTGGAAGAACTGGGGGAGGAGGAAGAGGAGGAGGAAGAGGAAGATGACTTTGAAGAAGAGGAGGAGGAAG
  AAGAAGGCCTGGGCCCCCCAGAGTCTGCCAGCCTGGGCACTGCAGGCCTGTTCACCCGCAAGGCCCCGCC
  CGCCCAGGCTTTCCGTGGAGACGGTGGTCCCAGGATGCTGAGTGGCCCTGAGCGTCTGGGACCTGGCCCA
  GCCCACCCCAGTCATATGGCATCCCAGATGCCACCACCGGACCATGGGGACTGGACCTTTGAGGAGCAGT
  TCAAACAGCTCTATGAACTGGATGCGGACCCCAAGAGGAAAGAGTTCCTGGATGACCTTTTCAGCTTCAT
  GCAGAAGCGGGGCACTCCAGTGAACCGGATCCCCATCATGGCGAAGCAGGTCCTCGACCTGTTCATGTTG
  TATGTGCTGGTGACCGAGAAGGGCGGCCTGGTAGAGGTTATCAACAAGAAACTGTGGAGGGAGATCACCA
  AGGGGCTCAACCTGCCTACCTCCATCACCAGTGCTGCCTTCACACTTCGGACACAGTACATGAAGTATCT
  TTACCCCTATGAGTGTGAGAGGCGAGGCCTGAGCAGCCCCAACGAGCTCCAGGCCGCCATAGACAGCAAT
  CGCAGGGAGGGCAGGCGCCAGAGCTTCGGTGGCTCACTCTTTGCCTACTCACCCAGTGGAGCCCACAGCA
  TGCTGCCCTCACCCAAGCTACCGGTGACTCCCCTGGGCCTCGCTGCCAGCACCAATGGCAGTTCCATCAC
  CCCAGCGCCCAAGATCAAGAAAGACTCGGCCATCCCCATCACAGTCCCAGGCCGCCTACCTGTATCTTTG
  GCAGGCCACCCTGTTGTCGCAGCCCAGGCTGCGGCGGTGCAAGCTGCGGCAGCCCAAGCAGCCGTTGCGG
  CCCAGGCAGCCGCCCTGGAGCAACTCCGGGAGAAGCTGGAATCCACAGAGCCTCCAGAAAAGAAGATGGC
  CCTAGTGGCCGATGAGCAACAGCGACTCATGCAGCGAGCTGTGCAGCAGAGCTTCCTGGCCATGACAGCC
  CAGCTGCCCATGAACATCCGCATCAACAGCCAAGCCTCTGAGAGCCGCCAGGACTCAGCCGTGAGTCTCA
  CCAGTGCCAACGGGAGCAACAGTATTAGCATGTCCGTGGAGATGAATGGTATTGTATACACAGGTGTGTT
  GTTCGCTCAGCCACCACCTCCTACAGCACCTTCCGCCCCCGGCAAAGGGGGCGTCAGCAGCATCGGTACC
  AACACCACCACGGGCAGCCGGACAGGAGCCAGTGGCAGCACTGTCAGCGGCGGCCAGGTGGGGCTGCCGG
  GGGTGTCTACACCCACCATGTCTTCTACCTCAAACAACTCCTTGCCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR209313 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MKLQAVMETLIQRQQRARQELEARQAPPPPPPEPTGVRARTTMTDEDREPENARMHRTQMAALAAMRAAA
  AGLGHPSSPGGSEDGPPISGDEDTAREGTLSSPALHGSVLEGAGHAEGDRHLMDVGSDDDDTKSKWEEQE
  LEELGEEEEEEEEEDDFEEEEEEEEGLGPPESASLGTAGLFTRKAPPAQAFRGDGGPRMLSGPERLGPGP
  AHPSHMASQMPPPDHGDWTFEEQFKQLYELDADPKRKEFLDDLFSFMQKRGTPVNRIPIMAKQVLDLFML
  YVLVTEKGGLVEVINKKLWREITKGLNLPTSITSAAFTLRTQYMKYLYPYECERRGLSSPNELQAAIDSN
  RREGRRQSFGGSLFAYSPSGAHSMLPSPKLPVTPLGLAASTNGSSITPAPKIKKDSAIPITVPGRLPVSL
  AGHPVVAAQAAAVQAAAAQAAVAAQAAALEQLREKLESTEPPEKKMALVADEQQRLMQRAVQQSFLAMTA
  QLPMNIRINSQASESRQDSAVSLTSANGSNSISMSVEMNGIVYTGVLFAQPPPPTAPSAPGKGGVSSIGT
  NTTTGSRTGASGSTVSGGQVGLPGVSTPTMSSTSNNSLP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007880
• ORF Size: 1800 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007880.1, NM_007880.2, NM_007880.3, NM_007880.4, NP_031906.1
• RefSeq Size: 5361 bp
• RefSeq ORF: 1806 bp
• Locus ID: 13496
• UniProt ID: Q62431
• Cytogenetics: 10 C1
• MW: 63.9 kDa
• Gene Summary: Transcription factor involved in B-cell differentiation. Binds a VH promoter proximal site necessary for induced mu-heavy-chain transcription. Binds the minor groove of a restricted ATC sequence that is sufficient for nuclear matrix association. This sequence motif is present in matrix-associating regions (MARS) proximal to the promoter and flanking E mu. Activates E mu-driven transcription by binding these sites. May be involved in the control of cell cycle progression by the RB1/E2F1 pathway.[UniProtKB/Swiss-Prot Function]