Plbd2 (NM_023625) Mouse Tagged ORF Clone

CAT#: MR209256

Plbd2 (Myc-DDK-tagged) - Mouse phospholipase B domain containing 2 (Plbd2)

Product Images

Circular map for MR209256

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Plbd2
• Synonyms: 1300012G16Rik; AU019810; P76
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR209256 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCCCCCGTGGATGGGAGCTCCGGCGGCTGGGCGGCCCGGGCGCTACGGCGGGCACTGGCGCTGA
  CCTCCCTGACCACACTGGCCTTGCTGGCCTCGCTGACCGGGCTGTTGCTGAGCGGCCCGGCGGGCGCTCT
  CCCTACCCTGGGGCCCGGCTGGCAGCGCCAAAATCCGGACCCGCCGGTCTCCCGCACCCGCTCGCTGCTG
  CTGGACGCCGCGTCGGGCCAGCTGCGCCTGGAGGATGGCTTCCACCCCGACGCGGTGGCCTGGGCCAACC
  TCACCAACGCCATCCGCGAGACCGGGTGGGCCTATCTGGACCTGAGCACAAATGGCAGGTACAATGACAG
  CCTGCAGGCCTATGCAGCTGGTGTGGTGGAGGCCTCTGTGTCTGAGGAGCTCATCTACATGCACTGGATG
  AACACGGTGGTCAACTACTGCGGCCCCTTCGAATACGAAGTCGGCTACTGTGAGAAGCTTAAGAACTTCC
  TGGAGGCCAACCTGGAGTGGATGCAGAGGGAAATGGAGCTTAACCCGGACTCTCCGTACTGGCACCAGGT
  GCGGCTGACCCTCCTGCAGCTGAAAGGCCTGGAGGACAGCTATGAAGGCCGTTTAACCTTCCCAACTGGG
  AGGTTCACCATCAAACCCTTGGGGTTCCTCCTGCTGCAGATCTCTGGAGACCTGGAAGACCTAGAGCCAG
  CCCTGAATAAGACCAACACCAAGCCTTCCCTGGGCTCCGGTTCATGCTCTGCCCTCATCAAGCTGCTGCC
  TGGCGGGCATGACCTCCTGGTGGCGCACAACACGTGGAACTCCTACCAGAACATGTTACGCATCATCAAG
  AAGTACAGGCTGCAGTTCCGGGAGGGGCCGCAAGAGGAGTACCCCCTGGTTGCTGGCAACAACTTGGTTT
  TCTCGTCCTACCCGGGCACCATCTTCTCCGGAGATGACTTCTACATCCTGGGCAGTGGCCTGGTCACCCT
  GGAGACCACCATTGGCAACAAGAACCCAGCCCTGTGGAAGTACGTGCAGCCCCAGGGCTGTGTGCTGGAG
  TGGATACGAAATGTCGTGGCCAACCGCCTGGCCTTGGACGGGGCCACCTGGGCAGACGTCTTCAAGCGGT
  TCAACAGCGGCACGTACAATAACCAGTGGATGATTGTGGACTACAAGGCATTCCTCCCCAACGGACCCAG
  CCCTGGAAGCCGGGTGCTCACTATCCTAGAACAGATCCCGGGCATGGTGGTGGTGGCTGACAAGACTGCA
  GAGCTCTACAAAACGACCTACTGGGCTAGCTACAACATCCCGTACTTTGAGACTGTATTCAACGCTAGTG
  GGCTGCAGGCCCTGGTGGCCCAGTATGGAGATTGGTTCTCTTACACTAAGAACCCTCGAGCCAAGATCTT
  CCAGAGGGACCAGTCACTGGTGGAGGACATGGACGCCATGGTCCGGCTCATGAGGTACAATGATTTCCTC
  CATGACCCTCTGTCATTGTGTGAAGCCTGCAACCCAAAGCCTAATGCGGAGAATGCCATCTCTGCCCGCT
  CTGACCTCAACCCCGCCAATGGCTCCTACCCATTTCAGGCCCTGCATCAGCGCGCCCATGGTGGCATTGA
  TGTGAAGGTGACCAGCTTTACCCTGGCCAAGTACATGAGCATGCTGGCGGCCAGTGGCCCCACGTGGGAC
  CAGTGTCCTCCGTTCCAGTGGAGCAAATCGCCATTCCACAGCATGCTGCACATGGGCCAGCCCGACCTCT
  GGATGTTCTCACCCATCAGGGTGCCATGGGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR209256 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAAPVDGSSGGWAARALRRALALTSLTTLALLASLTGLLLSGPAGALPTLGPGWQRQNPDPPVSRTRSLL
  LDAASGQLRLEDGFHPDAVAWANLTNAIRETGWAYLDLSTNGRYNDSLQAYAAGVVEASVSEELIYMHWM
  NTVVNYCGPFEYEVGYCEKLKNFLEANLEWMQREMELNPDSPYWHQVRLTLLQLKGLEDSYEGRLTFPTG
  RFTIKPLGFLLLQISGDLEDLEPALNKTNTKPSLGSGSCSALIKLLPGGHDLLVAHNTWNSYQNMLRIIK
  KYRLQFREGPQEEYPLVAGNNLVFSSYPGTIFSGDDFYILGSGLVTLETTIGNKNPALWKYVQPQGCVLE
  WIRNVVANRLALDGATWADVFKRFNSGTYNNQWMIVDYKAFLPNGPSPGSRVLTILEQIPGMVVVADKTA
  ELYKTTYWASYNIPYFETVFNASGLQALVAQYGDWFSYTKNPRAKIFQRDQSLVEDMDAMVRLMRYNDFL
  HDPLSLCEACNPKPNAENAISARSDLNPANGSYPFQALHQRAHGGIDVKVTSFTLAKYMSMLAASGPTWD
  QCPPFQWSKSPFHSMLHMGQPDLWMFSPIRVPWD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_023625
• ORF Size: 1785 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_023625.4, NP_076114.2
• RefSeq Size: 3595 bp
• RefSeq ORF: 1785 bp
• Locus ID: 71772
• UniProt ID: Q3TCN2
• Cytogenetics: 5 F
• MW: 66.3 kDa
• Gene Summary: Putative phospholipase.[UniProtKB/Swiss-Prot Function]